Chn irms isoprime 100 stable isotope ratio mass spectrometer

Manufactured by Elementar

Sourced in Italy

The CHN IRMS Isoprime 100 Stable Isotope Ratio Mass Spectrometer is a laboratory instrument designed for the precise measurement of the relative abundance of stable isotopes in various samples. It functions by ionizing and separating the different isotopes of elements such as carbon, hydrogen, and nitrogen, and then detecting and quantifying their relative proportions.

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Lab products found in correlation

Nexion 300, by PerkinElmer (1 mentions) Ultrawave, by Milestone (1 mentions)

2 protocols using chn irms isoprime 100 stable isotope ratio mass spectrometer

Grapevine Nutrient Profiling by ICP-OES and CHN-IRMS

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The element concentrations of several macro (P, K, Ca, Mg, S) and micro (Fe, Mn, Zn) nutrients in grapevine root and leaves samples were determined by Inductively Coupled Plasma–Optical Emission Spectroscopy (ICP-OES 5800. Agilent Technologies. Santa Clara. USA), while the total N and C were determined by CHN analyzer (CHN IRMS Isoprime 100 Stable Isotope Ratio Mass Spectrometer, Elementar Como Italy). For ICP analyses plant samples were oven-dried for 72 h (at 60–80 °C) and ground. For each sample, 100 mg of ground powder was ashed at 550 °C in glass vials and suspended in ultrapure HNO₃ as previously described by [21 (link)]. Element quantifications were carried out using certified multi-element standards. Regarding CHN analyses, plant leaves and roots were dried. And their total N and C contents were determined by CHN-IRMS (CHN IRMS Isoprime 100 Stable Isotope Ratio Mass Spectrometer, Elementar).

Khalil S., Strah R., Lodovici A., Vojta P., Berardinis F.D., Ziegler J., Pompe Novak M., Zanin L., Tomasi N., Forneck A, & Griesser M. (2024). The activation of iron deficiency responses of grapevine rootstocks is dependent to the availability of the nitrogen forms. BMC Plant Biology, 24, 218.

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Elemental Profiling of White Lupin

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The elemental composition of white lupin was analysed as previously described (Zanin et al., 2015 (link); Zanin et al., 2017 (link)) by inductively coupled plasma mass spectrometry (ICP‐MS NexION 300, PerkinElmer Inc.) and by CHN analyser (CHN IRMS Isoprime 100 Stable Isotope Ratio Mass Spectrometer, Elementar). For ICP analyses, shoots and roots were oven‐dried at 60°C and samples were acid‐digested with concentrated ultrapure HNO₃ (650 ml L⁻¹; Carlo Erba) using a single reaction chamber microwave digestion system (UltraWAVE, Milestone). Element quantifications were carried out using certified multi‐element standards (CPI International, https://cpiinternational.com).

Buoso S., Zamboni A., Franco A., Commisso M., Guzzo F., Varanini Z., Pinton R., Tomasi N, & Zanin L. (2022). Nodulating white lupins take advantage of the reciprocal interplay between N and P nutritional responses. Physiologia Plantarum, 174(1), e13607.

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