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Au400 chemistry analyzer

Manufactured by Olympus
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Sourced in Japan, France, United States

The AU400 is a chemistry analyzer manufactured by Olympus. It is designed to perform a variety of clinical chemistry tests on biological samples, such as serum, plasma, and urine. The AU400 utilizes proven analytical technology to provide accurate and reliable results.

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Lab products found in correlation

Umr 1149, by Olympus (4 mentions) Xt 2000i analyzer, by Olympus (3 mentions) Procyte dx hematology analyzer, by IDEXX (3 mentions) Mouse specific insulin and leptin elisa kit, by Merck Group (2 mentions) Mcytomag 70k, by Merck Group (2 mentions) Map2mag 76k, by Merck Group (2 mentions) Enzyme linked immunosorbent assay, by Fortis Life Sciences (1 mentions) Glucotrend 2, by Roche (1 mentions) Imx instrument, by Abbott (1 mentions) Crp ul assay, by Fujifilm (1 mentions) Au400, by Olympus (1 mentions) 7180 autoanalyzer, by Hitachi (1 mentions) Vet scan analyzer, by Abaxis (1 mentions) Ab83464, by Abcam (1 mentions) Ab83461, by Abcam (1 mentions) Ab102530, by Abcam (1 mentions) Elisa kit, by Thermo Fisher Scientific (1 mentions)

44 protocols using au400 chemistry analyzer

1

Enzymatic Assays for Metabolic Markers

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Fasting serum glucose concentration was determined by the enzymatic hexokinase method (glucose reagent, AU400 chemistry analyzer, Olympus). Fasting serum insulin concentration was determined by a microparticle enzyme immunoassay (IMx insulin reagent) on an IMx instrument (Abbott). Serum CRP concentration was analyzed using an AU400 chemistry analyzer (Olympus) and a highly sensitive turbidimetric immunoassay kit (CRP-UL-assay, Wako Chemicals, Neuss, Germany). The LOD was 0.02 mg/L. Interassay CVs were 3.33% at the mean level of 1.52 mg/L (n = 116) and 2.65% at the mean level of 2.51 mg/L (n = 168). Analyzed samples for fasting glucose, fasting insulin, HOMA-IR, and CRP concentrations were n = 1,951, n = 1,946, n = 1,938, and n = 1,952, respectively, for rs4251961 and n = 1,950, n = 1,945, n = 1,937, and n = 1,951, respectively, for rs6759676.
Herder C., Nuotio M.L., Shah S., Blankenberg S., Brunner E.J., Carstensen M., Gieger C., Grallert H., Jula A., Kähönen M., Kettunen J., Kivimäki M., Koenig W., Kristiansson K., Langenberg C., Lehtimäki T., Luotola K., Marzi C., Müller C., Peters A., Prokisch H., Raitakari O., Rathmann W., Roden M., Salmi M., Schramm K., Swerdlow D., Tabak A.G., Thorand B., Wareham N., Wild P.S., Zeller T., Hingorani A.D., Witte D.R., Kumari M., Perola M, & Salomaa V. (2014). Genetic Determinants of Circulating Interleukin-1 Receptor Antagonist Levels and Their Association With Glycemic Traits. Diabetes, 63(12), 4343-4359.
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2

Serum ALT Activity After CCl4 Treatment

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Blood was collected from intracardiac puncture on anesthetized mice during time-course kinetic after CCl4 treatment and the activity of serum alanine aminotransferase [ALT] was measured using the AU400 chemistry analyzer (Olympus) (Biochemistry Facility, CRI Institute, Paris, France).
Fortier M., Cadoux M., Boussetta N., Pham S., Donné R., Couty J.P., Desdouets C, & Celton-Morizur S. (2019). Hepatospecific ablation of p38α MAPK governs liver regeneration through modulation of inflammatory response to CCl4-induced acute injury. Scientific Reports, 9, 14614.
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3

Acetaminophen PK-PD in Malaria Patients

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Screening venous blood samples were analyzed for parasitemia and biochemistry (point-of-care iSTAT analyzer [CG4+, Chem8+], Abbott Laboratories, USA). After enrollment, vital signs, urine output, development of complications, and parasitemia were assessed every 6 hours until discharge or death. For the first 72 hours, serum creatinine was assessed every 12 hours (Olympus AU400 chemistry analyzer, performed in Bangkok), CFH in twice-centrifuged citrated plasma was assessed every 24 hours (enzyme-linked immunosorbent assay; Bethyl Laboratories, performed in Darwin) [9 (link)], and F2-IsoPs and IsoFs in lithium heparin plasma were assessed every 24 hours (gas chromatography-mass spectrometry at Vanderbilt University) [7 (link), 8 (link)]. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were assessed on enrollment and at 72 hours. To determine the PK–PD of acetaminophen on creatinine, parasitemia, and fever, plasma EDTA samples for acetaminophen concentration were collected prior to each dose plus dense sampling in the treatment arm after both the first (0 hour) and last (72 hour) dose. Patients in the control arm had samples collected every 6 hour for 72 hours to assess unplanned acetaminophen intake. Detailed procedures are provided in the Supplementary Material.
Plewes K., Kingston H.W., Ghose A., Wattanakul T., Hassan M.M., Haider M.S., Dutta P.K., Islam M.A., Alam S., Jahangir S.M., Zahed A.S., Sattar M.A., Chowdhury M.A., Herdman M.T., Leopold S.J., Ishioka H., Piera K.A., Charunwatthana P., Silamut K., Yeo T.W., Lee S.J., Mukaka M., Maude R.J., Turner G.D., Faiz M.A., Tarning J., Oates J.A., Anstey N.M., White N.J., Day N.P., Hossain M.A., Roberts II L.J, & Dondorp A.M. (2018). Acetaminophen as a Renoprotective Adjunctive Treatment in Patients With Severe and Moderately Severe Falciparum Malaria: A Randomized, Controlled, Open-Label Trial. Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, 67(7), 991-999.
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4

Analyzing Liver Enzyme Levels in Mice

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Blood samples were collected via the retroorbital route before sacrificing the mice. Aspartate aminotransferase (AST) and alanine transaminase (ALT) levels were determined by photometric enzyme activity assays with an Olympus AU400 Chemistry Analyzer using serum as described previously [9 (link), 20 (link)].
Dywicki J., Buitrago-Molina L.E., Noyan F., Schlue J., Iordanidis K., Manns M.P., Wedemeyer H., Jaeckel E, & Hardtke-Wolenski M. (2022). Splenectomy induces biochemical remission and regeneration in experimental murine autoimmune hepatitis. European Journal of Medical Research, 27, 284.
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5

Serum Collection for Biochemical Analysis

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Blood was collected from the abdominal aorta and centrifuged at 3000× g for 10 min to collect serum. The sera were immediately stored at −80 °C to analyze biochemical parameters, including BUN and SCr, using an Olympus AU400 chemistry analyzer (Tokyo, Japan).
Park Y.S., Han J.H., Park J.H., Choi J.S., Kim S.H, & Kim H.S. (2023). Pyruvate Kinase M2: A New Biomarker for the Early Detection of Diabetes-Induced Nephropathy. International Journal of Molecular Sciences, 24(3), 2683.
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6

Comprehensive Blood Biomarker Analysis in Mice

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On day 90, blood was collected from mice before euthanasia by submandibular puncture into tubes containing 5 µL EDTA 0.5 mol/L. After centrifugation (6000× g, 20 min, 4 °C), plasma was aliquoted and frozen at −80 °C until further analysis. Measurements of plasma AST, ALT, triglycerides (TG), high-density lipoprotein cholesterol (HDL), total cholesterol and ferritin were performed on the biochemistry platform (CRI, UMR 1149, Paris) with an Olympus AU400 Chemistry Analyzer. LDL (low-density lipoprotein) cholesterol was calculated according to the Friedewald formula:
Measurements of non-fasting plasma insulin and leptin were performed using a mouse-specific insulin and leptin ELISA Kit (Merck Millipore, Burlington, MA, USA).
Burz S.D., Monnoye M., Philippe C., Farin W., Ratziu V., Strozzi F., Paillarse J.M., Chêne L., Blottière H.M, & Gérard P. (2021). Fecal Microbiota Transplant from Human to Mice Gives Insights into the Role of the Gut Microbiota in Non-Alcoholic Fatty Liver Disease (NAFLD). Microorganisms, 9(1), 199.
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7

Quantifying HDL Cholesterol Levels

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HDL cholesterol was quantified using a standard colorimetric assay (Thermo DMA Co., San Jose, CA, USA) as previously described [12] (link). Samples were also assayed by the UCLA Clinical Laboratory for total cholesterol, high-density lipoprotein (HDL), non-HDL cholesterol and triglycerides (TG) using the Olympus AU400 Chemistry Analyzer.
Kelesidis T., Roberts C.K., Huynh D., Martínez-Maza O., Currier J.S., Reddy S.T, & Yang O.O. (2014). A High Throughput Biochemical Fluorometric Method for Measuring Lipid Peroxidation in HDL. PLoS ONE, 9(11), e111716.
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8

Blood Chemistry Analyses Using Automated Analyzers

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Analysis of blood chemistries was performed using a Sysmex XT-2000i analyzer for EDTA collected plasma, or an Olympus AU400 chemistry analyzer for serum.
Fears A.C., Beddingfield B.J., Chirichella N.R., Slisarenko N., Killeen S.Z., Redmann R.K., Goff K., Spencer S., Picou B., Golden N., Midkiff C.C., Bush D.J., Branco L.M., Boisen M.L., Gao H., Montefiori D.C., Blair R.V., Doyle-Meyers L.A., Russell-Lodrigue K., Maness N.J, & Roy C.J. (2022). Exposure modality influences viral kinetics but not respiratory outcome of COVID-19 in multiple nonhuman primate species. PLoS Pathogens, 18(7), e1010618.
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9

Blood Chemistry Analysis Protocols

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Analysis of blood chemistries was performed using a Sysmex XT-2000i analyzer for EDTA collected plasma, or an Olympus AU400 chemistry analyzer for serum.
Beddingfield B.J., Plante K.S., Plante J.A., Weaver S.C., Bose S., Krzykwa C., Chirichella N., Redmann R.K., Seiler S.Z., Dufour J., Blair R.V., Endt K., Volkmann A., Maness N.J, & Roy C.J. (2024). MVA-based vaccines are protective against lethal eastern equine encephalitis virus aerosol challenge in cynomolgus macaques. NPJ Vaccines, 9, 47.
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10

Comprehensive Urinalysis and Kidney Function Assessment

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Urinalysis performed by CIToxLAB included (1) quantitative measurements by using a Clinitek 500 urine analyzer/reflecto-spectrophotometer (Siemens) and a specific gravity refractometer (×1000), (2) semiquantitative measurements: proteins, glucose, ketones, bilirubin, nitrites, hemoglobin, urobilinogen, cytology of sediment by microscopic evaluation, and (3) qualitative parameters: appearance, color.
To evaluate kidney function at INSERM 1149, 10 µl of fresh urine were mounted on a Malassez slide to count the red blood cells (hematuria). Protein, albumin, and creatinine concentrations were measured in urine using the AU400 chemistry analyzer (Olympus). Neutrophil gelatinase-associated lipocalin (NGAL) and kidney injury molecule 1 (KIM-1) urinary concentrations were determined by ELISA using the corresponding kits (R&D Systems, Abingdon, UK). NGAL and KIM-1 are 2 biomarkers of early kidney dysfunction.
Coumoul X., Servien R., Juricek L., Kaddouch-Amar Y., Lippi Y., Berthelot L., Naylies C., Morvan M.L., Antignac J.P., Desdoits-Lethimonier C., Jegou B., Tremblay-Franco M., Canlet C., Debrauwer L., Le Gall C., Laurent J., Gouraud P.A., Cravedi J.P., Jeunesse E., Savy N., Dandere-Abdoulkarim K., Arnich N., Fourès F., Cotton J., Broudin S., Corman B., Moing A., Laporte B., Richard-Forget F., Barouki R., Rogowsky P, & Salles B. (2018). The GMO90+ Project: Absence of Evidence for Biologically Meaningful Effects of Genetically Modified Maize-based Diets on Wistar Rats After 6-Months Feeding Comparative Trial. Toxicological Sciences, 168(2), 315-338.
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