Fecal albumin measurements were performed on dried fecal pellets using the Mouse Albumin ELISA Quantitation Set from Bethyl Laboratories according to manufacturer’s instructions. An in vivo permeability assay to assess barrier function was performed using the FITC-labeled dextran method. Food and water were withdrawn overnight, and mice were gavaged with 45 mg/100 g body weight of FITC-labeled dextran (FD-4; Sigma-Aldrich). Serum was collected 5 hours after FD-4 gavage, and fluorescence intensity of each sample was measured (excitation, 492 nm; emission, 525 nm). Serum or plasma LPS concentrations were determined with the Mouse Lipopolysaccharides (LPS) ELISA Kit obtained from Cusabio following manufacturer’s instructions.
Mouse lipopolysaccharides lps elisa kit
Manufactured by Cusabio
The Mouse Lipopolysaccharides (LPS) ELISA Kit is a quantitative in vitro assay designed to measure the concentration of LPS in mouse samples. The kit utilizes the enzyme-linked immunosorbent assay (ELISA) technique to detect and quantify the target analyte.
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2 protocols using mouse lipopolysaccharides lps elisa kit
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Gastrointestinal Barrier Function Assays
2
Biomarkers of Liver Injury and Inflammation
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Serum aspartate transaminase (AST) and alanine transaminase (ALT) were measured using the In nity ALT Kit (Thermo Fisher Scienti c). Serum LPS was determined using the Mouse Lipopolysaccharides (LPS) ELISA Kit (Cusabio, Wuhan, China). The serum levels of TNF-α, IL-1β, and IL-6, as well as the hepatic triglyceride (TG) and hepatic lipid peroxidation (MDA) level, were determined using a Mouse ELISA Kit (Solarbio, Beijing, China). All the assays were performed in triplicate according to the manufacturer's instructions.
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