Dynamic laser scattering (DLS) using the N5 submicron Beckman Coulter particle size analyzer was used to measure the particle size distribution of the CNPs.
Particle size analyzer
Manufactured by Beckman Coulter
Sourced in United States
The Particle Size Analyzer is a laboratory instrument used to measure the size distribution of particles in a sample. It determines the size of particles by analyzing their interaction with light or other forms of radiation. The device provides accurate and reliable data on the particle size distribution, which is crucial for various industries and applications.
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Lab products found in correlation
Delsa nano software, by Beckman Coulter (2 mentions)
300 mhz instrument, by Bruker (1 mentions)
200 cx microscope, by JEOL (1 mentions)
Ft ir apparatus, by Bruker (1 mentions)
Jem 100cx, by JEOL (1 mentions)
Model 8400 s, by Shimadzu (1 mentions)
Model 50 50h, by Shimadzu (1 mentions)
Jsm 6490a, by JEOL (1 mentions)
Uv vis v 530, by Jasco (1 mentions)
Jem 2010, by JEOL (1 mentions)
Senterra raman spectrometer, by Bruker (1 mentions)
Jsm 6360la, by JEOL (1 mentions)
Ftir 8400s, by Bruker (1 mentions)
12 protocols using particle size analyzer
1
Particle Size Analysis of CNPs
2
Polymer Bead Synthesis Optimization
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EXAMPLE 5
Each reactor vessel was loaded with 30 grams of aqueous solution and 20 grams of non-aqueous solution and stirred with a pitch-blade impeller at 700 rpm. The mixture was heated at 1° C. per minute to 80° C. and held at 80° C. for 5 hours. The mixture was then heated gradually over 45 minutes to 92° C. and held at 92° C. for 60 minutes. The products were cooled to 25° C. and analyzed.
Each aqueous solution was PE2 (poly(AM-co-DADMAC)) at 1 wt % based on the weight of the aqueous solution, dissolved in tris buffer at either pH=7.5 or tris buffer at pH=8.5. Each non-aqueous solution was initiator and PE1 (poly(2-EHA-co-AA)) dissolved in a solvent of 90% styrene and 10% DVB by weight based on the weight of the solvent. The initiator was either t-BP (at 0.4 wt % of the t-BP stock solution, based on the weight of the non-aqueous solution) or BPO (at 0.3 wt % based on the weight of the non-aqueous solution). The amount of PE2 was 1 wt % based on the weight of the non-aqueous solution. The size of the resulting polymer beads was measured by light scattering using a Beckman-Coulter particle size analyzer and reported as the median diameter on a volume basis. Results were as follows:
3
UV Stress Assay for Microalgal Survival
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The microalgal cells collected from liquid cultures were spread onto agar plates using the top agar method followed by the UV stress treatment11 (link). Briefly, the cells in the early stationary phase, adjusted to approximately 500 cells/mL counted using a particle size analyzer (Beckman-Coulter Company, Indianapolis, IN, USA), were suspended in 0.2% agar. One mL of the suspended cells was transferred to an agar plate and spread to cover the whole plate. These agar plates covered with cells were irradiated with UV-B (302 nm, 6.5 mW/cm2) using a UV box (LM-20E Benchtop UV Transilluminator, UVP Company, Upland, CA, USA) for 1, 3 or 5 min, and then incubated under continuous 80 μmol photon/m2/s at 28 °C to until the colonies were clearly visible. The colonies were counted with the “analyze particles” function of the Image J software (Version 1.49 V). The survival rates of the UV stressed cells on the plates were estimated with the controls without UV treatment. The top agar method for spreading single cells was not suitable for the air-dried and the lyophilized cells because they formed small aggregates.
4
Particle Size Analysis of BSA and BSA/Au Solutions
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The particle size evolution in BSA and BSA/Au solutions was analyzed by dynamic light scattering (DLS) measurements in water on a Beckman Coulter particle size analyzer (Brea, CA, USA) using the Delsa Nano software (Beckman Coulter, Brea, CA, USA). The measurements were performed at room temperature.
5
Synthesis and Characterization of Au NPs
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All chemicals and solvents were purchased from Aldrich (Bucharest, Romania) or Chimopar (Bucharest, Romania). 1H- and 13C-NMR spectra were recorded on a Bruker 300 MHz instrument (Rheinstetten, Germany), using deuterated chloroform as the solvent and tetramethylsilane (TMS) as the internal standard. Infrared (IR) spectra were recorded on a Bruker FT-IR apparatus (Bremen, Germany). UV-Vis spectra were recorded in water at room temperature using an UVD-3500 double beam spectrophotometer (Labomed, LA, USA). Dinamic light scattering (DLS) analysis was performed in water on a Beckman Coulter particle size analyzer (Brea, CA, USA) using the Delsa Nano software (Beckman Coulter, Brea, CA, USA). Transmission electron microscopy (TEM) pictures were obtained using a Jeol 200 CX microscope (Jeol, Tokyo, Japan); a drop of diluted Au NPs was added on a 3 mm carbon copper grid and left to dry.
6
Characterization of Developed Nanoparticles
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The functional groups of the developed nanoparticles were determined by Fourier transform infrared spectroscopy (FT-IR, Model 8400 S, Shimadzu, Kyoto, Japan) and thermal examination was done by Thermogravimetric analyzer (TGA, Model 50/50H, Shimadzu). The morphological changes were investigated by a Transmission electron microscope (TEM, JEM-100CX, JEOL INC., Peabody, Kansas, USA). The average sizes of the formulated nanoparticles were estimated by a Particle size analyzer (Beckman, Coulter N5, Brea, CA., USA). Additionally, determination of the surface charges was achieved by the Zeta-Sizer (Malvern Panalytical Co., Royston, UK).
7
Characterization of ICAM-1 Targeted Nanobubbles
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Particle size and concentration of MBs were measured with particle size analyzer (Beckman Coulter, USA), and the average surface potential was measured with Zeta potentiometer (Malvern, England). The experiments were repeated three times. A fluorescence microscope (Olympus, Japan, 400x) was used to observe the binding of nanobubbles to ICAM-1, and a flow cytometer (Becton, Dickinson and Company, USA) was used to measure the binding rate of Biotin/FITC-ICAM-1 to nanobubbles.
8
Resveratrol Nanoemulsion Characterization
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The diameter and polydispersity index (PDI) of the resveratrol nanoemulsion were determined by means of a particle size analyzer (Beckman Coulter, California, USA) after five dilutions based on our previous study (Li et al., 2021 ).
9
Synthesis of Liposomal Lipopeptide-Copper Nanoparticles
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For the preparation of liposomal lipopeptide biosurfactant abetted CuNPs phosphatidylcholine and cholesterol (2:1 ratio) were taken into the round bottom flask linked to the rotary evaporator and the temperature was set into 37 °C. The phase of evaporation proceeded till the visualization of the dry film. The remaining solvent was recovered by vacuum evaporation for 1 h, subsequently, biosurfactant and CuNPs were added for the synthesis of EL-LP-CuNPs (Encapsulated liposomal lipopeptide abetted CuNPs). The contents were evaporated in the rotatory flask at 60 rpm for 30 min at room temperature and kept stagnantly for 2 h. The non-entrapped biosurfactant and CuNPs were detached by dialysis overnight by 1% DMSO. The particle size, polydispersity index, and zeta-potential of the liposomal preparation were obtained from DLS measurements by using a Beckman Coulter particle size analyzer through dynamic light scattering. For the visualization of liposomal vesicles, TEM investigations were carried out by negative staining with a 2% aqueous solution of phosphotungstic acid. The liposomal preparations were loaded and dried on a carbon-coated grid for staining prior visualization.
10
Synthesis and Characterization of Copper Nanoparticles
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Particles were synthesized by four-step chemical reductions with copper sulphate II pentahydrate as a precursor31 . The synthesized particles were examined using UV–vis absorption spectroscopy (Jasco UV–Vis V530) in the wavelength range of 450–800 nm. XRD analysis of CuNPs was performed on a diffractometer operated at 40 kV and 30 mA with Cu Ka radiation (1.54 Å) as a source. The scanning range of 2 h was between 10° to 80°. The elemental composition of the nanoparticle was studied by scanning electron microscopy (SEM, JEOL JSM-6490A) armed with an energy-dispersive X-ray spectrometer (EDX) (6490 LA). The particle size and zeta potential of the suspended particles were acquired by Beckman Coulter particle size analyzer through dynamic light scattering. The analysis was carried out at 25 °C and the polydispersity index (PDI) was sustained at 0.33 to ensure the appropriate distribution. Transmission electron microscopic (TEM) investigations were carried out on a JEM-2010 (JEOL) instrument equipped with a slow-scan CCD camera and at an accelerating voltage of 200 kV.
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