Cells were labeled with antibody or the corresponding isotype control in 1%BSA/PBS for 30 mins on ice. Cells were washed and analyzed using an Accuri C6 flow cytometer (BD Bioscience); histograms were generated using FlowJo software. Mean fluorescence values were normalized to the control cell line level. Antibodies used were CD82-Alexa-647 (Biolegend, ASL-24), CD53-PE (BioLegend, HI29), and Integrin β1-Alexa-647 (BioLegend, TS2/16). For active β1 integrin expression, cells were labeled with Ligand-induced binding site (LIBS) Anti-Integrin β1 Antibody (Millipore, HUTS-4) for 30 mins. Cells were washed and labeled with goat anti-mouse Alexa-647 secondary antibody (Invitrogen).
Integrin β1 alexa 647
Manufactured by BioLegend
Integrin β1-Alexa-647 is a fluorescently labeled antibody that binds to the integrin β1 subunit. Integrins are cell surface receptors that mediate cell-cell and cell-extracellular matrix interactions.
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Lab products found in correlation
2 protocols using integrin β1 alexa 647
1
Flow Cytometric Analysis of Cell Surface Markers
2
Flow Cytometric Analysis of Cell Surface Markers
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Cells were labeled with antibody or the corresponding isotype control in 1%BSA/PBS for 30 mins on ice. Cells were washed and analyzed using an Accuri C6 flow cytometer (BD Bioscience); histograms were generated using FlowJo software. Mean fluorescence values were normalized to the control cell line level. Antibodies used were CD82-Alexa-647 (Biolegend, ASL-24), CD53-PE (BioLegend, HI29), and Integrin β1-Alexa-647 (BioLegend, TS2/16). For active β1 integrin expression, cells were labeled with Ligand-induced binding site (LIBS) Anti-Integrin β1 Antibody (Millipore, HUTS-4) for 30 mins. Cells were washed and labeled with goat anti-mouse Alexa-647 secondary antibody (Invitrogen).
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