Il 7rα a019d5

Manufactured by Thermo Fisher Scientific

IL-7Rα (A019D5) is a lab equipment product manufactured by Thermo Fisher Scientific. It is an antibody that targets the Interleukin-7 Receptor Alpha (IL-7Rα) protein. The core function of this product is to facilitate the detection and analysis of IL-7Rα in various research and experimental applications.

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Lab products found in correlation

Cd19 sj25c1, by BD (2 mentions) Cd11c bu15, by Abcam (2 mentions) Cd3 sk7, by BD (2 mentions) Live dead violet, by Thermo Fisher Scientific (2 mentions) Cd123 fab301c, by R&D Systems (1 mentions) Crth2 bm16, by Miltenyi Biotec (1 mentions) Cd11b dcis1 18, by Abcam (1 mentions) Fcεri aer 37 cra 1, by BD (1 mentions) Cd45 h130, by BD (1 mentions) Cd45 hi30, by BD (1 mentions) Cd14 mφp9, by BD (1 mentions) Phospho iκbα, by Cell Signaling Technology (1 mentions)

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IL-7Rα (2 citations)

2 protocols using il 7rα a019d5

FACS Surface Staining of Immune Cells

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For FACS surface staining, the cells were labelled by the following anti-human antibodies: CD3 (SK7; BD), CD19 (SJ25C1; BD), CD123 (FAB301C; R&D Systems), CD11b (DCIS1/18), CD11c (BU15; Abcam), CD4 (A161A1, BD), CD8 (RPA-T8), FcεRI (AER-37 ([CRA-1]), CD14 (MϕP9; BD), CD45 (H130), CD56 (B159), CD45 (HI30, BD), CRTH2 (BM16; Miltenyi Biotec), IL-7Rα (A019D5), and live/dead violet (Invitrogen). The samples were acquired using FACSDiva on LSRFortessa flow cytometer. FlowJo software (FlowJo_v10.7.1) was used for data analysis.

Fonseka C.L., Hardman C.S., Woo J., Singh R., Nahler J., Yang J., Chen Y.L., Kamaladasa A., Silva T., Salimi M., Gray N., Dong T., Malavige G.N, & Ogg G.S. (2022). Dengue virus co-opts innate type 2 pathways to escape early control of viral replication. Communications Biology, 5, 735.

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FACS Staining of Human Immune Cells

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For FACS surface staining the cells were labelled by the following anti human antibodies purchased from Biolegend unless stated otherwise: CD3 (SK7; BD Biosciences), CD19 (SJ25C1; BD Biosciences), CD123 (FAB301C; R&D systems), CD11b (DCIS1/18), CD11c (BU15; Abcam), CD8 (RPA-T8), FcεRI (AER-37 (CRA-1)), CD14 (MφP9; BD biosciences), CD4 (MEM-241), CD45 (H130), ICOS (C398.4A), CD56 (B159), CRTH2 (BM16; Miltenyibiotec), IL-7Rα (A019D5), live/dead violet (L34955; Invitrogen), NKp30 (clone: AF29-4D12), NKp30 blocking antibody (Clone 210845 R&D systems, AF29-4D12 Miltenyi Biotec), Phospho-IκBα (Ser32/36 Cell Signalling 9246), Anti-B7-H6 antibody (ab121794), B7-H6 blocking antibody (17BL.3), CD68 (Y1/82A), Siglec-8 (7C9) and CD16 (3G8).

Salimi M., Xue L., Jolin H., Hardman C., Cousins D.J., McKenzie A.N, & Ogg G.S. (2015). Group 2 innate lymphoid cells express functional NKp30 receptor inducing type 2 cytokine production. Journal of immunology (Baltimore, Md. : 1950), 196(1), 45-54.

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