Dulbecco s modified eagle media

HEK293T and U2OS cells were cultured in Dulbecco’s Modified Eagle Media (Corning) supplemented with 10% (v/v) fetal bovine serum (Sigma) and 1 × Penicillin/Streptomycin (Corning). Cells were grown in 15 cm dishes and collected when they reached ~ 80% confluency. Pellets were washed in PBS and stored at −80 °C.

Pericytes were seeded at 8000 cells/mL into two 6‐well cell culture plate (Corning Inc., Corning, NY, USA) precoated with gelatin (Cell Biologics Inc.) in either Dulbecco's modified Eagle media (DMEM) with 4.5 g·L⁻¹ glucose, l‐glutamine, and sodium pyruvate (Corning Inc.) supplemented with +5% FBS (Corning Inc.) +1% penicillin/streptomycin (P/S) (Corning Inc.) or endothelial growth medium 2 (EGM‐2; Cell Biologics, Inc., Chicago, IL, USA) supplemented with the ECs medium supplement kit (Catalog M1168, Cell Biologics Inc., Chicago, IL, USA). Cells were seeded at 10 000 cells per well into a 24‐well cell culture plate (Corning) precoated with gelatin (Cell Biologics Inc.) in cell culture media. After 6 h for cells to attach, media was changed to include LDL (Sigma) or d‐glucose (Sigma). Plates were placed into an Incucyte Zoom instrument (EssenBioscience Inc., Ann Arbor, MI, USA) integrated with the incucyte zoom 2018A software (EssenBioscience Inc., Ann Arbor, MI, USA), and growth was monitored for 5 days to construct proliferation curves.

Human endometrial cancer cell lines AN3CA and ECC-1 have been described and authenticated by mitochondrial sequencing (7.1.11) [23 (link)]. Only short term cultures from the verified frozen stocks were used. The cells were maintained in Dulbecco's Modified Eagle media (Corning, Manassas, VA) supplemented with 10% fetal bovine serum (Omega Scientific, Tarzana, CA), 1% penicillin and streptomycin at 37°C in a humidified atmosphere of 5% CO₂.