Ab92308

For immunofluorescent staining, 7 μm acetone-fixed cryosections were thawed and then blocked for non-specific binding by incubation in PBS with 10% goat serum and casein solution, for 30 min at RT. This was followed by 1 h incubation with primary antibodies for SCARF-1 (8 μg/ml, Abcam ab92308) and CD31 (5 μg/ml, DAKO JC70A). Samples were washed three times in PBS followed by 30 min incubation with Alexa Fluor® conjugated secondary antibodies (1:500 dilution; Thermo Fisher Scientific). Nuclei were stained with 300 nM DAPI (Invitrogen) and slides were subsequently mounted with ProLong™ Gold Antifade Mountant (Invitrogen). Fluorescence images were acquired using a Zeiss 780 Zen confocal fluorescence microscope (ZEISS).

For immunofluorescent staining, 7 μm acetone-fixed cryosections were thawed and then blocked for non-specific binding by incubation in PBS with 10% goat serum and casein solution, for 30 min at RT. This was followed by 1 h incubation with primary antibodies against the following antigens: SCARF-1 (8 μg/ml, Abcam ab92308); CD31 (5 μg/ml, DAKO JC70A); αSMA (5 μg/ml, Sigma-Aldrich IA4); CD90 (1 μg/ml, eBioscience SE10); CK18 (1 μg/ml, DAKO DC10); EpCAM (5 μg/ml, Progen HEA125). Samples were washed three times in PBS followed by 30 min incubation with Alexa Fluor^® conjugated secondary antibodies (1:250; Thermo Fisher Scientific). Nuclei were stained with 300 nM DAPI (Invitrogen) and slides were subsequently mounted with Fluorescence Mounting Medium (DAKO). Fluorescence images were acquired using a Zeiss 780 Zen confocal fluorescence microscope (ZEISS).