Electrophoretic mobility shift assay gel shift kit

Hydrangenol was purchased from Coresciences Co. (Seoul, South Korea). Antibodies against CDK2, CDK4, cyclin D1, cyclin E, p21^WAF1, p27^KIP1, p53, and GAPDH were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Total ERK, p38, JNK, AKT, phospho-ERK, phospho-p38, phospho-JNK, and phospho-AKT antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA). The polyclonal MMP-9 antibody was purchased from Chemicon (Temecula, CA, USA). The nuclear extract kit and the electrophoretic mobility shift assay (EMSA) gel shift kit were obtained from Panomics (Fremont, CA, USA). The p38-specific inhibitor SB203585 was purchased from Calbiochem (San Diego, CA, USA).

Resveratrol (≥98%, analytical standard grade) was purchased from Sigma–Aldrich (St. Louis, MO, USA). Polyclonal antibodies against extracellular signal-regulated kinase ERK (9102S), phospho-ERK (9101S), p38 mitogen-activated protein kinase (9212S), phospho-p38 MAPK (9211S), Jun N-terminal-kinase (9258S), phospho-JNK (9251S), AKT (9272S), phospho-AKT (9272S), and p21WAF1 (2947S) were obtained from Cell Signaling Technology Inc. (Danvers, MA, USA). Polyclonal antibodies against CDK2 (sc-163), cyclin E (sc-377100), CDK4 (sc-23896), cyclin D1 (sc-8396), p53 (sc-1641), p27KIP1 (sc-1641), β-actin (sc-47778), 5α-reductase 2 (sc-293232), AR (sc-7305), FGF-2 (sc-1360), B-cell lymphoma-2 (sc-7382) and Bcl-2-associated x protein (sc-20067) were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). A nuclear extract kit and electrophoretic mobility shift assay (EMSA) gel shift kit were obtained from Panomics (Fremont, CA, USA). Polyclonal antibodies against PARP-1 (sc-7150), caspase-9 (sc-7885), caspase-7 (9492S), caspase-3 (sc-7148), cleaved PARP-1 (sc-7150), cleaved caspase-9 (sc-7885), cleaved caspase-7 (9492S), cleaved caspase-3 (sc-7148), and XIAP (sc-11426) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA, USA).

Anti-extracellular signal-regulated kinase (ERK), anti-phospho-ERK, anti-p38 mitogen-activated protein kinase (MAPK), anti-phospho-p38 MAPK, anti-Janus kinase (JNK), anti-phospho-JNK, anti-protein kinase B (AKT), and anti-phospho-AKT antibodies were purchased from Cell Signaling Technology Inc. (Danvers, MA, USA). Polyclonal anti-cyclin D1, anti-cyclin E, anti-cyclin-dependent kinase (CDK) 2, anti-CDK4, anti-p53, anti-p21WAF1, anti-p27KIP1, and anti-GAPDH antibodies were obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, CA, USA). Moreover, a Ki-67-specific antibody was obtained from Invitrogen (Waltham, MA, USA). Moreover, a nuclear extract kit and electrophoretic mobility shift assay (EMSA) gel shift kit were purchased from Panomics (Fremont, CA, USA). SP600125 were purchased from Calbiochem (San Diego, CA, USA).