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Chondroitin sulfate from shark

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Chondroitin sulfate from shark is a naturally-derived compound used in various laboratory applications. It is a sulfated glycosaminoglycan found in the cartilage of sharks and other marine organisms. This product can be utilized in cell culture, biochemical assays, and research applications that require this specific biomolecule.

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Lab products found in correlation

Anhydrous cacl2, by Merck Group (1 mentions) Chloroform, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) 1 9 dimethylmethylene blue, by Merck Group (1 mentions)

Spelling variants of this product

Chondroitin sulfate (134 citations) Shark chondroitin sulfate (35 citations) Chondroitin sulfate from shark cartilage (16 citations) Chondroitin sulfate sodium salt from shark cartilage (7 citations) Chondroitin-6-sulfate from shark (5 citations) Chondroitin 6-sulfate from shark cartilage (2 citations)

2 protocols using chondroitin sulfate from shark

1

DPPC Lipid Extraction Protocol

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In all experiments, Milli-Q water (18.2 MΩ cm) was used, and it is further referred to as “water”. Chloroform (spectroscopic grade, >99%), methanol (spectroscopic grade, >99.9%), anhydrous CaCl2, and chondroitin sulfate from shark cartilage (CS) were purchased from Sigma. Dipalmitoyl phosphatidylcholine (DPPC) was purchased from Avanti. Every chemical was used without further purification.
Szekeres G.P., Krekic S., Miller R.L., Mero M., Pagel K, & Heiner Z. (2021). The interaction of chondroitin sulfate with a lipid monolayer observed by using nonlinear vibrational spectroscopy. Physical Chemistry Chemical Physics, 23(23), 13389-13395.
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2

Extraction and Quantification of Proteoglycans

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The PGs were extracted with 4 M guanidinium hydrochloride as described in our previous studies [6 (link), 8 (link)]. The extracted PGs were precipitated in 70% ethanol, dissolved in water, and glycosaminoglycan (GAG) contents were quantitated using 1,9-dimethylmethylene blue (Sigma-Aldrich) assay [14 (link)]. Chondroitin sulfate from shark (Sigma) was used as a standard. Separation of the extracted PGs in a 1.2% agarose gel and staining with Toluidine Blue was performed as previously described [6 (link)].
Ylärinne J.H., Qu C, & Lammi M.J. (2017). Scaffold-free approach produces neocartilage tissue of similar quality as the use of HyStem™ and Hydromatrix™ scaffolds. Journal of Materials Science. Materials in Medicine, 28(4), 59.
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