Rat rrv00 quantikine kit

Manufactured by R&D Systems

The Rat/RRV00 Quantikine kit is a quantitative sandwich enzyme immunoassay designed for the measurement of rat RRV00 concentrations in cell culture supernates, serum, and plasma.

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Lab products found in correlation

Proteinase inhibitor, by Merck Group (2 mentions) Cold lysis buffer, by Cell Signaling Technology (1 mentions)

2 protocols using rat rrv00 quantikine kit

Quantifying IL-6 and VEGF in Mouse/Rat Tissues

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Serum and tissue levels of IL-6 (Mouse/M6000B and Rat /R6000B Quantikine kit, R&D Systems Inc., Minneapolis, MN) and VEGF (Rat/RRV00 Quantikine kit, R&D Systems) were determined using an enzyme-linked immunosorbent assay (ELISA) kit according to manufacturer’s instructions. Briefly, flash-frozen liver tissue was homogenized in a cold lysis buffer (Cell Signaling Technology Inc., Beverly, MA) consisting of a 0.1% proteinase inhibitor (Sigma-Aldrich). The homogenates were then centrifuged at 14,000 rpm for 20 min at 4°C, and the total protein concentration was determined using a bichinchoninic acid method (BCA) (Sigma-Aldrich). Undiluted serum was used. IL6 and VEGF values were then normalized to protein concentration. All samples and standards were measured in duplicate, and the average value was recorded as pg per mL [37 (link),38 (link)].

Ahmed M., Kumar G., Navarro G., Wang Y., Gourevitch S., Moussa M.H., Rozenblum N., Levchenko T., Galun E., Torchilin V.P, & Goldberg S.N. (2015). Systemic siRNA Nanoparticle-Based Drugs Combined with Radiofrequency Ablation for Cancer Therapy. PLoS ONE, 10(7), e0128910.

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Cytokine and Growth Factor Analysis

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Serum and tissue samples (periablational and/or distant tumor, where specified) were harvested at 6hr after treatment for IL-6 and 72hr after treatment for VEGF and HGF, as previous studies have demonstrated peak concentrations at these times (10 , 14 (link), 26 (link)). For liver samples, tissues were specifically harvested from the periablational rim. Samples were assayed for IL-6 (rat/R6000B Quantikine kit, R&D Systems), HGF (rat/MHG00 Quantikine kit, R&D Systems Inc. Minneapolis, MN) and VEGF (rat/RRV00 Quantikine kit, R&D Systems) using an enzyme-linked immunosorbent assay (ELISA) kit according to the manufacturer’s instructions. Flash-frozen liver and tumor tissue was homogenized in a cold lysis buffer (Cell Signaling Technology Inc., Beverly, MA) consisting of 0.1% proteinase inhibitor (Sigma-Aldrich). The homogenates were then centrifuged at 14,000 rpm for 20 minutes at 4°C, and the total protein concentrations were determined using a bichinchoninic acid method (BCA) (Sigma-Aldrich). IL-6, VEGF, and HGF values were then normalized to protein concentration. Undiluted serum was used. All samples were measured in duplicate, and the average value was recorded (9 ).

Ahmed M., Kumar G., Gourevitch S., Levchenko T., Galun E., Torchilin V, & Goldberg S.N. (2018). Radiofrequency ablation (RFA) induced systemic tumor growth can be reduced by suppression of resultant heat shock proteins. International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group, 34(7), 934-942.

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