Ferric chloride (FeCl3), anhydrous sodium acetate (NaOAc), and hexahydrate nickel chloride (NiCl2·6H2O) were purchased from Shantou Xilong Chemical Factory (Guangdong, China). Dimercaptosuccinic acid (DMSA), phosphotungstic acid (TPA), dicyclohexyl carbodiimide (DCC) and MTX were purchased from Sigma-Aldrich (ST. Louis, USA), N-hydroxysuccinimide (NHS) were purchased from Solarbio (Beijing, China). Diethylene glycol (DEG), glycerol, and dimethyl sulfoxide (DMSO) were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China). Urea, glycine, tris(hydroxymethyl) aminomethane (Tris), 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and Annexin V-FITC/PI Apoptosis Detection Kit were purchased from KeyGen Biotech Co. Ltd. (Nanjing, China). RPMI 1640 culture medium and penicillin-streptomycin were purchased from Biological Industries (Beit Ahemeq, Israel). Fetal bovine serum (FBS) and trypsin were purchased from Invitrogen (California, USA). All chemicals were used without further purification. Ultrapure water (18.2 MΩ·cm) were obtained from Milli-Q Water Purification System.
N hydroxysuccinimide nhs
Manufactured by Solarbio
Sourced in China
N-hydroxysuccinimide (NHS) is a chemical compound commonly used as an activating agent in organic synthesis. It is a white crystalline solid that is soluble in various organic solvents. NHS is primarily used to facilitate the formation of amide bonds between carboxylic acids and primary amines.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin streptomycin, by Sartorius (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Sinopharm (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by Keygen Biotech (1 mentions)
Rpmi 1640 culture medium, by Sartorius (1 mentions)
3 4 5 dimethylthiazol 2 yl 2, by Keygen Biotech (1 mentions)
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Tianjin Kemiou Chemical Reagent (1 mentions)
Trypsin edta solution, by Beyotime (1 mentions)
N n carbonyldiimidazole cdi, by Maclin Biochemical Technology (1 mentions)
Doxorubicin hydrochloride dox, by Meilun (1 mentions)
1 ethyl 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Solarbio (1 mentions)
Tunel kit, by Beyotime (1 mentions)
Alexa fluor 488 labeled goat anti rabbit secondary antibody, by Abcam (1 mentions)
Dipalmitoylphosphatidylcholine, by Avanti Polar Lipids (1 mentions)
Rat anti mouse cd31 monoclonal antibody, by Abcam (1 mentions)
1 3 dimethylaminopropyl 3 ethylcarbodiimide hydrochloride edc, by Merck Group (1 mentions)
6 protocols using n hydroxysuccinimide nhs
1
Synthesis and Characterization of DMSA-coated Nanoparticles
2
Doxorubicin-loaded SWCNT Nanocarriers
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SWCNTs with high purity were purchased from Chengdu Organic Chemicals Co. Ltd., Chinese Academy of Sciences. Polyethyleneimine (PEI, Mw = 600 Da) was purchased from Aladdin Chemistry Co., Ltd. Doxorubicin hydrochloride (DOX) was purchased from Dalian Meilun Biological Technology Co., Ltd (Dalian, China). Cell culture medium RPMI 1640, fetal bovine serum (FBS), penicillin/streptomycin solution and fluorescent Hoechst 33342 were obtained from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Trypsin–EDTA solution (0.25% trypsin with 0.02% EDTA) was supplied by Beyotime Biotechnology (Beijing, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St. Louis, MO). NHS-PEG-COOH (Mw = 5 kDa) was purchased from Shanghai Ponsure Biotechnology Co., Ltd. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide (NHS) were purchased from Beijing Solarbio Science & Technology Co., Ltd. N,N′-Carbonyldiimidazole (CDI) was purchased from Shanghai Macklin Biochemical Technology Co., Ltd. Dicyclohexylcarbodiimide (DCC) was purchased from Aladdin. Ultrapure water was produced with a Milli-Q water system. Dimethyl sulfoxide (DMSO) was produced from Tianjin Kemiou Chemical Reagent Co., Ltd. All other reagents were of analytical grade.
3
Synthesis of Silk-Based Hydrophobic Coatings
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Fresh cocoons of Bombyx mori silkworm were obtained from College of Biotechnology, Southwest University. Sodium carbonate anhydrous (Na2CO3), calcium chloride anhydrous (CaCl2), and N,N-dimethylformamide (DMF) were purchased from Kelong Chemical Reagent Factory (Chengdu, China) and at AR grade. Anhydrous ethanol (CH3CH2OH, AR grade) was purchased from Chongqing Chuandong Chemical Group Co., Ltd. (Chongqing, China). 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) were purchased from Solarbio (Beijing, China). Anhydrous dimethyl sulfoxide (DMSO), dibutyltin dilaurate and hydroquinone were purchased from Aladdin Industrial Co., Ltd. (Shanghai, China). 2-Isocyanatoethyl methacrylate (MOI) was purchased from Meryer Chemical Technology Co., Ltd. (Shanghai, China). 1H,1H,2H,2H-perfluorodecanethiol (PFDT) and 1H,1H,2H,2H-perfluoro-1-decanol (FTOH) were purchased from Sigma-Aldrich (Saint-Louis, USA). Ultrapure water (resistance > 18 MΩ cm−1) was used in all experiments.
4
Paclitaxel-Loaded Nanoparticle Formulation
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Fetal bovine serum, RPMI1640 medium, and trypsin were purchased from HyClone (Logan Utah, USA). Dipalmitoylphosphatidic acid, dipalmityl phosphatidylglycerol, and dipalmitoylphosphatidyl ethanolamine were purchased from CordenPharma (Corden Pharma, Liestal, Switzerland). Dipalmitoylphosphatidylcholine was purchased from Avanti Polar Lipids, Inc. (Alabaster, AL, USA). The crosslinking product between 1,2-distearoyl-phosphatidylethanolamine and polyethylene glycol conjugate-2000 was purchased from NANOCS (Boston, MA, USA). 1,1ʹ-dioctadecyl-3,3,3ʹ,3ʹ-tetramethylindocarbocyanine perchlorate (DiI), 4′,6-diamidino-2-phenylindole (DAPI), CCK-8 reagents and a TUNEL kit were purchased from Beyotime Biotechnology (Shanghai, China). Paclitaxel (PTX), carbodiimide (EDC), and N-hydroxysuccinimide (NHS) were purchased from Solarbio (Beijing, China). AMD070 was purchased from Absin Biotechnology (Shanghai, China). Rabbit anti-human CXCR4 monoclonal antibody, rat anti-mouse CD31 monoclonal antibody, FITC-labeled rabbit anti-rat secondary antibody, and Alexa Fluor 488-labeled goat anti-rabbit secondary antibody were purchased from Abcam (Cambs, UK). An Annexin V Apoptosis Detection Kit FITC kit was purchased from Thermo Fisher Scientific (Waltham, MA, USA). BALB/c-nu female nude mice were purchased from Huafukang Bioscience Co., Inc. (Beijing, China).
5
Immunization Protocol for BALB/c Mice
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Six-week-old female Bagg Albino (BALB/c) mice were purchased from SLAC Laboratory Animal Company (Shanghai, China). Mouse myeloma cells (SP2/0) were obtained from the National Collection of Authenticated Cell Cultures of China (Shanghai, China). Carrier proteins of keyhole limpet hemocyanin (KLH) and N-hydro-xysuccinimide (NHS) were obtained from Solarbio Biotechnology Company (Beijing, China). 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) was obtained from Sigma Aldrich (St. Louis, MO, USA). Fenvalerate (analytical standard) was purchased from Dr. Ehrenstorfer GmbH (Augsburg, Germany). All other chemicals were of analytical grade, unless otherwise specified.
6
Decellularized Porcine Carotid Artery Scaffolds
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The decellularized porcine carotid artery scaffolds were prepared as described in our previous study [17 (link),18 (link)]. Briefly, Carotid arteries from porcine (internal diameter 3 mm, length 5 cm) were obtained, the lumen was flushed with heparin saline to remove blood clots, the outer membrane and fatty tissue were stripped, the vessels were washed with phosphate buffered saline (PBS) for one day, the vessels were shocked in 1% Triton X-100 solution (Labest Biological Technology, Beijing, China) for 24 hours (100 r/min), shocked with 0.3% sodium dodecyl sulfate (SDS, Hanran Biological Technology, Shanghai, China) for 72 hours and changed daily. Decellularised vessels were washed with PBS solution to remove residual detergent. The decellularised vessels were finally cross-linked using N-(3-dimethylaminopropyl)-N0ethylcarbodiimide hydrochloride (EDC) and N-hydroxysuccinimide (NHS) to enhance mechanical properties and immobilize heparin sodium (Beijing Solarbio Science & Technology, China). The vessels were finally dispensed and disinfected using Y-rays.
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