The distal ileums (1-cm-long segment) were removed and homogenized in RIPA buffer (Beyotime Institute of Biotechnology) containing 1% protease inhibitor. The homogenates were centrifuged at 24,170 × g for 10 min at 4°C and the supernatants were collected and stored at −80°C. The concentrations of protein were detected using a BCA protein assay kit (Beyotime Institute of Biotechnology). The protein expression levels of tumor necrosis factor-α (TNF-α), interleukin (IL)-1β and IL-17A in the plasma and the distal ileum tissues were measured using ELISA kits (eBioscience; cat. nos. BMS622, BMS630 and BMS635 for TNF-α, IL-1β and IL-17A, respectively; Thermo Fisher Scientific, Inc.) as previously described (29 (link)).
Bms622
Manufactured by Thermo Fisher Scientific
Sourced in United States, Austria
The BMS622 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for general-purpose use in research and analytical laboratories. The core function of the BMS622 is to provide a controlled and consistent environment for various laboratory applications. Detailed specifications and intended use may vary, so further information should be obtained from the manufacturer.
Automatically generated - may contain errors
Lab products found in correlation
Bms630, by Thermo Fisher Scientific (4 mentions)
Bms625, by Thermo Fisher Scientific (4 mentions)
Bms621, by Thermo Fisher Scientific (2 mentions)
Ripa buffer, by Beyotime (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Bms635, by Thermo Fisher Scientific (1 mentions)
Cytokine elisa kit, by Thermo Fisher Scientific (1 mentions)
Bms629, by Thermo Fisher Scientific (1 mentions)
Reverse transcription kit, by Thermo Fisher Scientific (1 mentions)
Cat kit, by Nanjing Jiancheng (1 mentions)
Sod kit, by Nanjing Jiancheng (1 mentions)
Mda kit, by Nanjing Jiancheng (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Ab61224, by Abcam (1 mentions)
Ab32518, by Abcam (1 mentions)
Ab190503, by Abcam (1 mentions)
Lps escherichia coli o111 b4, by Merck Group (1 mentions)
Ab193297, by Abcam (1 mentions)
Ab40854, by Abcam (1 mentions)
Ab137550, by Abcam (1 mentions)
12 protocols using bms622
1
Inflammatory Cytokine Quantification in Distal Ileum
2
Cytokine Profiling of Colon Samples
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For assessing the colon concentrations of IL-6, IL-10, TNF-α and IFN-γ, approximately 1 cm from colonic samples was cut into longitudinal segments and cleaned with PBS with penicillin/streptomycin. After that, serum-free RPMI 1640 medium with penicillin/streptomycin was utilized for the culturing of colon sections for 24 h. In the next step, cell-free supernatants were evaluated for cytokine secretion using Thermo Fisher cytokine ELISA kits (BMS625 BMS629, BMS622 and BMS621, respectively).
3
Cytokine Quantification via ELISA
4
ELISA-Based TNF-α Quantification
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Serum TNF-α level was measured using enzyme-linked immunosorbent assay (ELISA) kit (Bender MedSystems, Catalog number BMS622, Vienna, Austria). Absorbance of the samples was measured by VERSA brand (designed by Molecular Devices in California, USA) microplate reader at 450 nm. Results were given as picogram/mL [10 (link)].
5
Measuring Inflammatory Cytokines by ELISA
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Concentrations of TNF-α and IL-6 in the supernatant were measured by enzyme-linked immunosorbent assay (BMS622, BMS603, Bender MedSystems, Austria) according to the manufacturer’s instructions.
6
Quantification of Inflammatory Cytokines
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The supernatant was collected from the RD and Vero cells following the different treatments. The protein levels of interleukin-beta (IL-1β, BMS224; Invitrogen), IL-6 (BMS213, Invitrogen), and tumor necrosis factor-alpha (TNF-α, BMS622; Invitrogen) were determined using ELISA kits (Invitrogen™) following the manufacturer’s instructions. quantified using their corresponding standard curves.
7
Plasma Cytokine Analysis after MI
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Two weeks after MI and patch treatment, blood samples were collected and transferred to EDTA tubes. The blood samples were centrifuged for 10 min at 25 °C, and plasma was stored at −80 °C until assay. Tumor necrosis factor (TNF-α) and interleukin-6 (IL-6) in plasma were quantified using ELISA kits (BMS622 and BMS625, Invitrogen, Waltham, MA, USA), according to the manufacturer's instructions.
8
Quantifying Brain Inflammation Biomarkers
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Interleukin-6 (IL-6), interleukin 1β (IL-1β), and tumor necrosis factor-alpha (TNF-α) were colorimetrically detected in the brain tissue according to the manufacturers' instructions based on the sandwich and competitive ELISA technique using commercially available kits for rat IL-6 (BMS625), (IL-1β) (BMS630), and TNF-α (BMS622), (Invitrogen, Thermo Fisher Scientific, MI, USA). IL-6, IL-1β, and TNF-α concentrations were measured in brain homogenate as pg/ml at 450 nm by interpolation from a standard curve via a micro-plate ELISA reader (Sorin Biomedica SpA., Milan, Italy) with a detection limit from 31.3–2000 pg/ml, 31.3–2000 pg/ml, and 39.1–2500 pg/ml, respectively (Jiang et al. 2021 (link)).
9
Quantifying Cytokine Levels in Serum
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To detect IL-1β or TNF-α in blood serum, we used a solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) Kit (eBioscience, San Diego, CA, USA; BMS630 or BMS622). Sera were prepared at the indicated times, and standard samples were diluted with distilled water and applied to ELISA plates. IL-1β or TNF-α concentrations were determined according to the manufacturer’s protocol. Absorbance levels were measured at 450 nm using an ELISA reader.
10
Erythropoietin and Antifibrotic Effects
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The following experimental reagents and drugs were obtained: adenine (Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd., 151029), anti-EPO antibody (Abcam, ab61224), anti-EPOR antibody (Abcam, ab61162), anti-TGF-β1 polyclonal antibody (AbSci, #AB41494), anti-α-SMA antibody (Boster, BM0002), Siwu granules (Jitai'an (Sichuan) Pharmaceutical Co., Ltd., Chinese medicine prescription: Z20020016), recombinant human erythropoietin injection (Shenyang Sansheng Pharmaceutical Co., Ltd., Chinese medicine prescription: S20010001), MDA Kit (Nanjing Jiancheng Bioengineering Institute, A003-1), CAT Kit (Nanjing Jiancheng Bioengineering Institute, A007-1), NO Kit (Nanjing Jiancheng Bioengineering Institute, A013-2), SOD Kit (Nanjing Jiancheng Bioengineering Institute, A001-3), Trizol (Invitrogen, 103106), reverse transcription kit (Thermo Fisher Scientific, #K1662), amplification kit (Roche, 04913914001), and IL-6 (eBioscience, BMS625) and TNF-α (eBioscience, BMS622) ELISA kits.
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