Goat anti human igg fc pe secondary antibody

Manufactured by Thermo Fisher Scientific

Goat anti-human IgG Fc-PE secondary antibody is a laboratory reagent used to detect and quantify the presence of human immunoglobulin G (IgG) in samples. It is a polyclonal antibody produced in goats and conjugated to the fluorescent dye phycoerythrin (PE), which allows for the visual identification and measurement of target IgG molecules.

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Lab products found in correlation

Live dead aqua dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Goat anti rabbit igg hrp conjugate, by Merck Group (1 mentions) Lsr 2 flow cytometer, by BD (1 mentions)

Spelling variants of this product

Anti-human IgG-PE (6 citations) Anti-human IgG-Fc PE (4 citations) Goat anti-human Fc-PE (3 citations) Goat anti-human IgG-Fc secondary antibody (2 citations) Goat-anti-human IgG Fc-PE (2 citations) Goat anti-human IgG Fc (2 citations) Anti-human IgG Fc antibody (2 citations)

2 protocols using goat anti human igg fc pe secondary antibody

Antibody-based ELISA and In Vitro Binding Assays

Cited 2 times

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The following antibodies were used for ELISAs: goat anti-rabbit IgG HRP conjugate (Millipore) and rabbit anti-monkey IgG HRP conjugate (Sigma) were used as detection antibodies for measuring anti-gp120 IgG titers.
The following antibodies and staining reagents were used for the in vitro-binding studies: goat anti-human IgG Fc-PE secondary antibody (Invitrogen); LIVE/DEAD Aqua Dead Cell Stain Kit (Life Technologies); CH65;⁵⁵ (link) and HIV nAbs CH58,¹⁴ (link) CH106,¹⁵ (link) and 17b.¹⁶ (link)

Pardi N., LaBranche C.C., Ferrari G., Cain D.W., Tombácz I., Parks R.J., Muramatsu H., Mui B.L., Tam Y.K., Karikó K., Polacino P., Barbosa C.J., Madden T.D., Hope M.J., Haynes B.F., Montefiori D.C., Hu S.L, & Weissman D. (2019). Characterization of HIV-1 Nucleoside-Modified mRNA Vaccines in Rabbits and Rhesus Macaques. Molecular Therapy. Nucleic Acids, 15, 36-47.

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Quantification of Antibody Binding

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4 × 10⁵ transfected cells were incubated at 4°C for 1 h with 30 μg/mL nAb, then washed in PBS with 1% BSA. Cells were then incubated at 4°C for 30 min with goat anti-human IgG Fc-PE secondary antibody (2.5 μg/mL, Invitrogen). Finally, cells were incubated with a LIVE/DEAD differential stain (Life Technologies), following the manufacturer’s instructions, and washed in PBS with 1% BSA. The presence of PE-labeled antibody on cell surfaces was detected on a BD LSRII flow cytometer. At least 10,000 events for each sample were recorded and data were analyzed with the FlowJo 10 software.

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