Cells were tested using a Mycoplasma detection kit (C0296, Beyotime, Hangzhou, China) according to the manufacturer's instructions. The assay was based on Hoechst 33,258 staining of Mycoplasma DNA, which was observed and photographed using a fluorescence microscope (Axio Observer, Zeiss).
Mycoplasma detection kit
Manufactured by Beyotime
Sourced in China
The Mycoplasma Detection Kit is a laboratory equipment designed to detect the presence of mycoplasma contamination in cell cultures. It provides a reliable and efficient method for the identification of mycoplasma species.
Automatically generated - may contain errors
Lab products found in correlation
Axio observer, by Zeiss (1 mentions)
Skov3 cells, by American Type Culture Collection (1 mentions)
Balb c nude mice, by GemPharmatech (1 mentions)
Hek293t, by American Type Culture Collection (1 mentions)
Beas 2b, by American Type Culture Collection (1 mentions)
Hsaec1 kt, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Hcc1937, by Sartorius (1 mentions)
Mda mb 231, by Sartorius (1 mentions)
Mcf 7, by Sartorius (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Mitosox, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Bodipy 493 503, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions)
Penicillin and streptomycin, by Corning (1 mentions)
B16f10 murine melanoma cell line, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
10 protocols using mycoplasma detection kit
1
Mycoplasma Detection Using Hoechst Staining
2
Establishing Cancer Cell Lines and Xenografts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
SKOV3 cells (human ovarian cancer cell line), human renal proximal convoluted tubule epithelial cells HK2 and human peritoneal mesenchymal cells HMrSVS were purchased from American Type Culture Collection (ATCC, USA) in May 2016. All cells were routinely tested for mycoplasmas contamination using a mycoplasma detection kit (Beyotime, China) before used. SKOV3, HK2 and HMrSVS cultured in McCoy’s 5A, DMEM/F12 and RPMI 1640 medium with 10% fetal bovine serum (FBS) and 1% pen/strep solution in a humidified atmosphere of 5% CO2 and 95% air at 37 °C. Firefly luciferase labeled SKOV3 cells (SKOV3-Luc) was generated as previously described [18 (link)]. Five-week-old to six-week-old female BALB/c nude mice (18–22 g) were purchased from GemPharmatech Co., Ltd (Guangzhou, China). All animal experiments were approved by the Animal Care and Use Committee (SYSU-IACUC-2021-B0815) of Sun Yat-sen University.
3
Culturing Liver Cell Lines for Research
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human liver cancer cell lines HepG2, BEL-7404, BEL-7402, QGY-7701 and Hep3B were obtained from the Chinese Type Culture Collection (CTCC, Shanghai, China). The cells were cultured in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. The Human normal liver cell line L02 (CTCC, Shanghai, China) was maintained in 10% FBS-supplemented DMEM medium and 1% Penicillin/Streptomycin. All the cell lines were incubated under standard conditions at 37 °C in a humidified atmosphere of 5% CO2. The cells were passaged twice or thrice a week and discarded after 20 passages. All cells have been tested negative for mycoplasma contamination using Mycoplasma Detection Kit (Beyotime Biotechnology, Guangzhou, China).
4
Characterization of Diverse Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Human nonsmall cell lung carcinoma A549 and Calu‐1, immortalized human bronchial epithelial cells (Beas‐2B), human small airway epithelial cells (HSAEC1‐KT) and human embryonic kidney 293T cells (HEK293T) were obtained from the American Type Culture Collection (Rockville, MD, USA). All cell lines were used within 10–20 passages according to the ATCC recommendation. Cell lines were routinely tested for mycoplasma contamination using a mycoplasma detection kit (Beyotime, Guangzhou, China). All cell lines were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum and maintained in a 37 °C, 5% CO2 incubator if not specified.
5
Breast Cancer Tissue Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Formaldehyde-fixed paraffin-embedded (FFPE) BC tissues and unpaired mammary hyperplasia (non-tumor tissues) were randomly collected from patients who had undergone surgery at the Shaanxi Provincial People's Hospital in China. Clinicopathological data such as age and gender, as well as histological data, tumor size, lymph node metastasis status, ER status, PR status, and AR status were obtained by reviewing their pathology records. Specimens were collected after obtaining written informed consent from the patients as well as approval of the ethical committees. Patient anonymity was maintained throughout the study. Human BC cell lines MCF-7, HCC1937, MDA-MB-231 and MDA-MB-435, human breast epithelium cells HBL-100 [23 (link)] and human embryonic kidney (HEK) 293T cells were obtained from the Cell Bank (Shanghai Institute of Biochemistry and Cell Biology, CAS, Shanghai, China).Cells were maintained in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (Biological Industries) and 1% antibiotics (100 U/mL penicillin and 100 mg/mL streptomycin sulfate). Cells were grown in 5% CO2 at 37 °C. The cell line was tested for mycoplasma contamination using the Mycoplasma Detection Kit (Beyotime, Haimen, China) and was found to be negative.
6
Cellular Mitochondrial Assessment
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Dulbecco’s modified Eagle’s medium (DMEM), Lipofectamine RNAiMax, penicillin and streptomycin were from Invitrogen (Carlsbad, CA). Fetal bovine serum (FBS) was from Gibco (Carlsbad, CA). MitoSOX, DCF, TMRM, BODIPY™ 500/510, BODIPY™ 493/503 were from Molecular Probes (Eugene, OR). Mycoplasma contamination was detected using Mycoplasma Detection Kit (Beyotime, China).
7
Culturing Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The ES2, SKOV3, and HEK293T cancer cell lines were kindly provided by Dr. Jiaxing Yang (Peking Union Medical College Hospital). All the cell lines were maintained in DMEM medium supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin at 37°C with 5% CO2. All the cell lines tested negative for mycoplasma using a Mycoplasma Detection kit (C0301S, Beyotime).
8
Osteosarcoma Cell Line Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The osteosarcoma cell line used in this study was SW1353, which was purchased from the American Typical Biological Resources Collection (ATCC). The cell culture medium consisted of 90% Dulbecco's modified Eagle's medium (DMEM) + 10%FBS + 1% penicillin–streptomycin. Cells were maintained at 37 °C in a constant temperature incubator with 5% CO2. The cells were detected for mycoplasma contamination using the Mycoplasma detection kit (Beyotime, China) according to the protocal.
9
Culturing Melanoma, Lung Carcinoma, and HUVEC Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
B16F10 murine melanoma cell line and Lewis lung carcinomas (LLC) cell line were obtained from the American Type Culture Collection. B16F10 and LLC cells were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum and 100 U/mL penicillin and streptomycin (Corning, NY, USA) at 37 °C in an incubator with 5% CO2. Human umbilical vein endothelial cells (HUVECs) were isolated and purified from the donated umbilical cords as previously described with minor modification29 (link), as approved by the Medical Ethical Committee of Jiangsu Province Hospital on Integration of Chinese and Western Medicine (permit and approval number: 2021-LWKY-003). Written informed consent was obtained. HUVECs were passaged with trypsin-ethylenediaminetetraacetic acid and used at low passage numbers between 2 and 5. In the hypoxia experiment, HUVECs were maintained under an atmosphere of 1% O2 for 12 h in the absence or presence of SAA and then used for further analysis. All cells used in this study were negative for mycoplasma as periodically tested by a mycoplasma detection kit (Beyotime, Shanghai, China).
10
Cell Culture Protocol for Liver Cancer Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human liver cancer cell lines HepG2 and Bel-7404 were obtained from the Chinese Type Culture Collection (CTCC, Shanghai, China). The cells were cultured in DMEM medium with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. The Human normal liver cell line L02 (CTCC, Shanghai, China) was maintained in 10% FBS-supplemented DMEM medium and 1% Penicillin/Streptomycin. All the cell lines were incubated under standard conditions at 37°C in a humidi ed atmosphere of 5% CO2. The cells were passaged twice or thrice a week and discarded after 20 passages. All cells have been tested negative for mycoplasma contamination using Mycoplasma Detection Kit (Beyotime Biotechnology, Guangzhou, China).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!