Dulbecco s modified eagle s medium dmem

Tryptic soy agar (TSA), maximum recovery diluent (MRD), tryptone soya broth (TSB), and plate count agar (PCA) were purchased from VWR (Radnor, PA, USA).
Trypsin-ethylenediaminetetraacetic acid (Trypsin-EDTA) 0.25%/1 mM EDTA 4Na in HBSS, w/o:Ca and Mg, w:Phenol red and Dulbecco’s modified Eagle’s medium (DMEM) with 4.5 g/L glucose and 2 mM L-glutamine were purchased from PanBiotech (Aidenbach, Germany). Fetal bovine serum heat inactivated (FBS) was purchased from Biowest (Nuaille, France) and 1% antibiotic-antimycotic solution from Corning (Corning-New York, NY, USA) as well as phosphate-buffered saline (PBS) 10× Molecular Biology Grade. Sodium dodecyl sulfate (CAS No. 151-21-3) was purchased from Merck (Darmstadt, Germany). Triton X-100 (CAS No. 9002-93-1) and Neutral Red (CAS No. 553-24-2) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Lactate dehydrogenase (LDH) cytotoxicity detection kit and water-soluble tetrazolium (WST-1) cell proliferation reagent (CAS No.150849-52-8) were bought from Roche (Basel, Switzerland). CLF was purchased from Respol (Leiria, Portugal).

Primary human DSF, hBM-MSC and hAd-MSC deriving from consenting normal donors have been previously isolated in our laboratory and were retrieved to be used in the current study from our established cell bank. Primary human DSF have been isolated from dermal tissue explants, as described before [24 (link)]; primary hBM-MSC have been established after the immunomagnetic isolation of BM-CD105⁺ cells using Milteny microbeads according to the manufacturer’s instructions (Miltenyi Biotech, Bergisch Gladbach, Germany) [25 (link)]; primary hAd-MSC have been established after incubation of adipose tissue with 1.0 mg/mL of collagenase for 1 h. hBM-MSC and hAd-MSC have been characterized based on their ability for osteogenic, chondrogenic and adipogenic differentiation. Cells were routinely cultured in Dulbecco’s modified Eagle’s medium (DMEM) of 4.5 mg/mL and 1.0 mg/mL glucose for human DSF and hBM-MSC/hAd-MSC, respectively (PAN-Biotech, Aidenbach, Germany), supplemented with penicillin (100 U/mL), streptomycin (100 mg/mL) (obtained from Biosera, Nuaille, France) and 10% (v/v) fetal bovine serum (FBS) (from Gibco BRL, Invitrogen, Paisley, UK) in a humidified atmosphere of 5% CO₂ and 37 °C. Cells were subcultured either when confluent (human DSF) or at 80% confluency (hBM-MSC and hAd-MSC) using a trypsin/citrate (0.25%/0.30% w/v) solution.

N-Methyl-4-piperidone, Benzaldehyde, Diisopropylamine, n-Butyllithium (2.5 M solution in hexanes), 4-(Dimethylamino)pyridine (DMAP), and Sodium bicarbonate were purchased from Merck. Acetic acid, Dichloromethane, Tetrahydrofuran, Ethanol, Ethyl acetate, Citric acid, Sodium Sulfate(VI), and Acetic anhydride were purchased from POCH, Gliwice, Poland. Vanilin was purchased from Acros, Waltham, Massachusetts, USA. Dimethyl-sulfoxide (DMSO), Potassium superoxide, 18-crown-6-ether, Nitro blue tetrazolium chloride (NBT), Curcumin, Fetal bovine serum (FBS), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), recombinant PTP1B phosphatase, para-nitrophenyl phosphate (pNPP), and 2′,7′-dichlorofluorescein were purchased from Sigma-Aldrich, Saint Louis, Missouri, USA. Dulbecco’s Modified Eagle’s Medium (DMEM) and Phosphate-buffered saline (PBS) were purchased from PAN-biotech, Aidenbach, Germany. Lastly, 4–20% MP TGX Tain-Free Gel 10W was purchased from Bio-Rad Laboratories, Hercules, CA, USA and PTP1B antibodies were purchased from Cell Signaling, Danvers, MA, USA.

Cell culture flasks were obtained from Greiner (Frickenhausen, Germany). Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal calf serum (FCS) were purchased from PAN-Biotech (Aidenbach, Germany). Phosphate-buffered saline (PBS), medium supplements for LS180 cell culture (glutamine, non-essential amino acids, penicillin/streptomycin), penicillin–streptomycin (100×), and zosuquidar were purchased from Sigma-Aldrich (Taufkirchen, Germany). Rifampicin was from Applichem (Darmstadt, Germany), rifabutin was purchased Toronto Research Chemicals (North York, Canada). Rhodamine 123 was purchased from CalBiochem (Darmstadt, Germany). The Absolute QPCR SYBR Green Mix was supplied by Abgene (Hamburg, Germany).

MS-grade water, organic solvents, and formic
acid (FA) were purchased from Biosolve Chimie SARL (Dieuze, France).
2dGO, O6-m2dGO, 2′-deoxy-N-ethyl-guanosine (2dNetGO), d3-O6-methyl-2′-deoxyguanosine
(d3-O6-m2dGO), N7-mG, O6-mG, and N2-methyl-2′-deoxyguanosine
(N2-m2dGO) were purchased from Toronto Research Chemicals (North York,
Canada) (Supporting Information, Figure S1). Nucleoside Digestion Mix NEB #M0649 was purchased from New England
Biolabs (New England Biolabs, Ipswich, MA, USA). Cell culture medium,
Dulbecco’s Modified Eagle’s Medium (DMEM), and fetal
calf serum (FCS) were purchased from PAN-Biotech (Aidenbach, Germany).
Glutamine and penicillin/streptomycin were purchased from Sigma-Aldrich
(Taufkirchen, Germany). Crystal violet was purchased from Applichem
(Darmstadt, Germany). The bromodeoxyuridine (BrdU) assay kit was purchased
from Merck (Darmstadt, Germany). The DNA extraction kit was purchased
from Qiagen (Hilden, Germany).

Enisamium iodide and N-methyl-4-N-ethanolpyridinium iodide (internal standard for LC-MS/MS) were provided by Farmak JSC.
Dulbecco’s Modified Eagle’s Medium (DMEM) was obtained from Pan Biotech (Aidenbach, Germany). [³H]-propranolol, rhodamine 123, Krebs-Ringer buffer (KRB), cyclosporine A and [³H]-digoxin from Sigma-Aldrich. Trypsin/ethylenediaminetetraacetic acid (EDTA) solution and sodium dodecyl sulfate from Merck (Darmstadt, Germany). Fluorescein was purchased from VWR International (Darmstadt, Germany). Culture flasks were obtained from VWR International and Transwell filter inserts from Corning Life Sciences (Lowell, MA, USA).