Jem 1100

Manufactured by JEOL

Sourced in Japan

The JEM-1100 is a transmission electron microscope (TEM) manufactured by JEOL. It is designed to provide high-resolution imaging and analysis of samples at the nanoscale level. The JEM-1100 utilizes an electron beam to magnify and image the internal structure of materials, enabling the observation of fine details and the study of the atomic-scale properties of a wide range of specimens.

Automatically generated - may contain errors

Lab products found in correlation

Formvar carbon coated tem grid, by Ted Pella (2 mentions) Jem 2200f, by JEOL (1 mentions) Carbon coated copper grid, by Ted Pella (1 mentions) Lead citrate, by Polysciences (1 mentions) Polybed 812 epoxy resin, by Polysciences (1 mentions) C3h6o, by Merck Group (1 mentions) Glutaraldehyde, by Merck Group (1 mentions) Sodium cacodylate, by Merck Group (1 mentions) Osmium tetraoxide, by Merck Group (1 mentions)

5 protocols using jem 1100

Transmission Electron Microscopy of Nanocrystals

Check if the same lab product or an alternative is used in the 5 most similar protocols

The samples were prepared by dropping
dilute solutions of NCs onto carbon coated gold grids, which were
then placed under a vacuum to preserve them from oxidation. Low resolution
transmission electron microscopy (TEM) measurements were carried out
on a JEOL JEM-1100 transmission electron microscope operating at an
acceleration voltage of 100 kV. High resolution TEM (HRTEM) was performed
with a JEOL JEM-2200FS microscope equipped with a 200 kV field emission
gun, a CEOS spherical aberration corrector in the objective lens,
enabling a spatial resolution of 0.9 Å, and an in column energy
filter. High angle annular dark field images were acquired on the
same microscope in scanning mode (STEM) with a nominal probe size
of 0.2 nm, and an inner cutoff angle of the annular detector of 75
mrad.

Tu R., Xie Y., Bertoni G., Lak A., Gaspari R., Rapallo A., Cavalli A., Trizio L.D, & Manna L. (2016). Influence of the Ion Coordination Number on Cation Exchange Reactions with Copper Telluride Nanocrystals. Journal of the American Chemical Society, 138(22), 7082-7090.

+ Open protocol

+ Expand

Visualizing Particle Trimer Nanostructures

Check if the same lab product or an alternative is used in the 5 most similar protocols

TEM was used to control the correct assembly of the particle trimer nanostructures. A droplet of the solution containing the purified structures was deposited on a plasma-exposed carbon-formvar-coated TEM grid (Ted Pella) and then dabbed off after 3 minutes. The grid was stained with 1% uranyl formate for 15 seconds. Imaging was performed with a JEOL JEM-1100 at an acceleration voltage of 80 kV.

Roller E.M., Besteiro L.V., Pupp C., Khorashad L.K., Govorov A.O, & Liedl T. (2017). Hot spot-mediated non-dissipative and ultrafast plasmon passage. Nature physics, 13(8), 761-765.

+ Open protocol

+ Expand

Polyplexes Characterization via TEM

Check if the same lab product or an alternative is used in the 5 most similar protocols

Polyplexes (pDNA concentration 10 μg/mL) were formed in water instead of HBG. The preparation of carbon-coated copper grids (Ted Pella, USA, 300 mesh, 3.0-mm OD) and the staining procedure was performed as described previously.⁴⁶ All grids were analyzed with a JEOL JEM-1100 (JEOL, Tokyo, Japan) electron microscope at 80 kV acceleration voltage.

Spellerberg R., Benli-Hoppe T., Kitzberger C., Hageneier M., Schwenk N., Öztürk Ö., Steiger K., Multhoff G., Eiber M., Schilling F., Weber W.A., Kälin R.E., Glass R., Nelson P.J., Wagner E, & Spitzweg C. (2022). Dual EGFR- and TfR-targeted gene transfer for sodium iodide symporter gene therapy of glioblastoma. Molecular Therapy Oncolytics, 27, 272-287.

+ Open protocol

+ Expand

Visualizing Particle Trimer Nanostructures

Check if the same lab product or an alternative is used in the 5 most similar protocols

Roller E.M., Besteiro L.V., Pupp C., Khorashad L.K., Govorov A.O, & Liedl T. (2017). Hot spot-mediated non-dissipative and ultrafast plasmon passage. Nature physics, 13(8), 761-765.

+ Open protocol

+ Expand

Ultrastructural Analysis of Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells (5 × 10⁵ per well) were placed in 6-well flat-bottom plates (Corning®) with 2 mL of supplemented medium (Gibco™) for 24 h. Adherent cells were treated with IC₅₀ of samples and controls for 24 h. Then, the culture medium was removed, and cells were fixed with 2.5% glutaraldehyde (Sigma®) for 24 h at 25 °C. Next, cells were washed with 0.1 M sodium cacodylate (Sigma®) (pH 7.2), and post-fixed with 1% osmium tetraoxide (Sigma®) for 1 h at 25 °C. Subsequently, cells were dehydrated with EtOH: propylene oxide (C₃H₆O, Sigma®) (50, 70, 90, 100% EtOH and 100% C₃H₆O, v/v) for 10 min at 4 °C. The inclusion of the cells was done with Poly/Bed® 812 epoxy resin (Polysciences®) at 60 °C for 24 h. Finally, ultrathin sections (60 nm thickness) were obtained with an ultramicrotome (Porter-Blum MT-1, Sorvall®). The slices were contrasted with 2% uranyl acetate (Polysciences®) for 20 min, and 0.2% lead citrate (Polysciences®) for 5 min. The observation of preparations was carried out in a TEM (JEM-1100, Jeol™) [30 ].

Varela-Rodríguez L., Sánchez-Ramírez B., Hernández-Ramírez V.I., Varela-Rodríguez H., Castellanos-Mijangos R.D., González-Horta C., Chávez-Munguía B, & Talamás-Rohana P. (2020). Effect of Gallic acid and Myricetin on ovarian cancer models: a possible alternative antitumoral treatment. BMC Complementary Medicine and Therapies, 20, 110.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!