Anti cep55

Manufactured by Santa Cruz Biotechnology

Anti-CEP55 is a laboratory reagent used for research purposes. It is a protein that specifically binds to and detects the presence of the CEP55 protein in biological samples.

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Cep55 (4 citations)

2 protocols using anti cep55

Protein Expression Analysis in Glioblastoma Cells

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U87 and T98G cells were lysed in radioimmunoprecipitation assay buffer [150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% sodium dodecyl sulfate (SDS), 50 mM Tris, pH 8.0, 5.0 mM ethylenediaminetetraacetic acid, pH 8.0, 0.5 mM dithiothreitol and 1 mM phenylmethylsulfonyl fluoride]. Protein concentrations were determined using the bicinchoninic acid method (Thermo Scientific, Rockford, IL, USA). Protein lysates were separated by SDS-polyacrylamide gel electrophoresis and transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA) by electroblotting. Primary antibodies for immunodetection were anti-CEP55 (Santa Cruz), anti-GLUT1 (Santa Cruz), anti-p-Akt^S473 (Santa Cruz), anti-p-Akt^T308(Santa Cruz), anti-Akt (Santa Cruz), anti-p-mTOR (Santa Cruz), anti-mTOR (Santa Cruz), anti-BAD (Santa Cruz), anti-caspase-9 (Santa Cruz), anti-GSK3-β (Santa Cruz), anti-p27 (Abcam) and anti-GAPDH (Santa Cruz). Subsequent to being incubated with Horseradish peroxidase (HRP) conjugated anti-rabbit or anti-mouse secondary antibodies (1: 10000, Santa Cruz) for 1 h, the immune complexes were detected using the enhanced chemiluminescence method.

Wang G., Liu M., Wang H., Yu S., Jiang Z., Sun J., Han K., Shen J., Zhu M., Lin Z., Jiang C, & Guo M. (2016). Centrosomal Protein of 55 Regulates Glucose Metabolism, Proliferation and Apoptosis of Glioma Cells via the Akt/mTOR Signaling Pathway. Journal of Cancer, 7(11), 1431-1440.

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Immunofluorescence Analysis of Cytoskeletal Proteins

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Primary mouse antibodies used were anti-γ-Tubulin (1:100; Sigma, clone GTU-88) and anti-GM130 (1:100; BD Bioscience). Primary rabbit antibodies used were anti-MKLP1 (1:1000, Santa Cruz Biotechnology, clone N-19), anti-ZO-1 (1:200; Invitrogen) and anti-PKCζ (1:200;Santa Cruz Biotechnology). Primary rat antibody used was anti-integrin α₆ (1:100; Millipore). Primary goat antibody used was anti-Cep55 (1:100; Santa Cruz Biotechnology). Alexa fluorophore-conjugated secondary antibodies (1:1000 for all secondary antibodies; Invitrogen) or rhodamine-phalloidin (1:1000; Invitrogen) and Hoechst to label nuclei (10 μg/mL) were used. The procedure for the immunofluorescence staining and image data acquisition and analysis was described previously in detail.¹⁸ (link)

Lujan P., Rubio T., Varsano G, & Köhn M. (2017). Keep it on the edge: The post-mitotic midbody as a polarity signal unit. Communicative & Integrative Biology, 10(4), e1338990.

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