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9 protocols using lauric acid

1

Synthesis of Copper Nanoparticles

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Copper nitrate hexahydrate (Cu(NO3)2⋅3H2O, 99%, Sinopharm Chemical Regent Co., Ltd), sodium borohydride (NaBH4, 98%, Sinopharm Chemical Regent Co., Ltd), trimesic acid (C9H6O6, 99%, Adamas-beta), lauric acid (C12H24O2, 99%, Adamas-beta), 1-butanol (C4H10O, 99.9%, Adamas-beta), potassium hexacyanoferrate (III) (K3[Fe(CN)6], 99%, Adamas-beta) and ethanol (C2H5OH, 99.5%, Adamas-beta) were used as received. Millipore water was used in all experiments.
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2

Jatropha curcas Seed Oil Extraction

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Oleic
acid (AR), methanol
(AR, >99%), lauric acid (AR, 98%), myristic acid (AR, 98%), palmitic
acid (AR, 98%), stearic acid (AR, 98%), phosphomolybdic acid (AR),
and iron(III) chloride hexahydrate (FeCl3·6H2O, AR) were purchased from Sinopharm Chemical Regent Co., Ltd. All
other chemicals were of analytical grade and used as received, unless
otherwise noted. Jatropha curcas seeds were purchased from Luodian
County, Guizhou Province, and the Jatropha curcas crude oil (JCCO)
was obtained through mechanical expression.
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3

Polymeric Nanoparticle Formulation for Drug Delivery

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Poly (butyl methacrylate-co-(2-dimethylaminoethyl) methacrylate-co-methyl methacrylate) (p (BMA-co-DAMA-co-MMA), Eudragit E100) was perchased from Shanghai Dexiang Medicine Tech. Co., Ltd. Poly (lactic-co-glycolic acid) (PLGA) (50:50, Mw 10,000) was purchased from Jinan Daigang Biomaterial Co., Ltd., China. Hydrogenated coconut glyceride lipid (HCG lipid, Softisan 100) was purchased from Sasol (China) Co., Ltd., Nanjing, China. Dimethyl carbonate (DMC, >98%) was purchased from TCI (Shanghai) Development Co., Ltd., China. Polyvinyl alcohol (PVA, Mw 1750 ± 50), 1-tetradecanol, 1-hexadecanol, lauric acid, sodium dodecyl sulfate (SDS), Span 80 and dichloromethane (>99.5%) were purchased from Sinopharm Chemical Reagent Corporation, Shanghai, China. EM 90 was purchased from Evonik Industries, Shanghai, China. All of the above reagents were used as received without further purification, and deionized water was used throughout the experiment.
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4

Lipase-Catalyzed Biodiesel Production

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Lipases from the Rhizopus oryzae (ROL) and Burkholderia cepacia (BCL) were purchased from Sigma-Aldrich. Polyethyleneimines with 1800 molecular weight, sebacoyl chloride (SC), cyclohexane, Span 80 and bovine serum albumin (BSA) were brought from Shenshi Chemical Industry. Rhodamine B (RhB), sodium fluorescein (Flu), FITC (fluorescein isothiocyanate), FITC-dextran (4 K and 10 K), and reference standards of fatty acid methyl esters were commercially obtained from Sigma-Aldrich. Other reagents, such as acetone, lauric acid, laurinol, ethanol, isooctane, tert-butanol, hexane, Na2HPO4, NaH2PO4, and NaOH were of analytical grade and got from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). Soybean oil was taken from local market and its physical properties are: saponification value 183.8 mg/g, acid value 0.23 mg/g, average mean molecular weight 917. The fatty acid compositions are listed in Table 1. The water used in all the experiments is purified with a water purification system and with a resistivity higher than 18.2 MΩcm. The carbon nanotubes used (CNTs) in the microcapsules are multiwalled with 20–40 nm for bore diameter, less than 5 μm for length.
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5

Synthesis and Characterization of Fluorescent Probes

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The ACQ probes, P2 (λabs/λem = 708 nm/732 nm) and P4 (λabs/λem = 651 nm/662 nm), were synthesized in the lab. Oleic acid, decanoic acid, lauric acid, myristic acid, palmitic acid, and stearic acid were purchased from Sinopharm Reagent Co., Ltd. (Shanghai, China). Cremophor EL, choline hydroxide, linOleic acid, and linolenic acid were obtained from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China). Paclitaxel and docetaxel were from Meilun Biotechnology Co., Ltd. (Dalian, China). Diamidino-2-phenylindole (DAPI) for nuclear staining was purchased from Yeasen Biotech Co., Ltd. (Shanghai, China). Caco-2 and Raji cells were from National Collection of Authenticated Cell Cultures (Shanghai, China), while HT29-MTX cells were from China Center for Type Culture Collection (Wuhan, China).
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6

Lipase Immobilization on Functionalized MWCNTs

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Rhizopus oryzae lipase (ROL) and Candida rugosa lipase (CRL) powders were commercially purchased from Sigma-Aldrich (Shanghai, China). Multi-walled carbon nanotubes (MWCNTs, 60–100 nm in diameter, with >95% purity) were bought from Nanotech Port Co., Ltd. (Shenzhen, China). Four-arm polyethylene glycol amine (4-arm-PEG-NH2, 10K) was obtained from Shanghai Zhenzhong Biotech Co., Ltd. (Shanghai, China). Lauric acid, N-ethyl-N-(3-(dimethylamino) propyl) carbodiimide hydrochloride (EDC), glutaraldehyde (GA), and other chemicals were procured from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Waste cooking oil (WCO) was thankfully donated by ZTE Agrivalley Co., Ltd. (Hubei, China), and its composition is shown in Figure S1 and Table S1.
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7

Synthesis of Fatty Acid-Based Compounds

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Oleic acid (AR) , methanol (AR, >99%) , lauric acid (AR, 98%) , stearic acid (AR, 98%) , myristic acid (AR, 98%) , palmitic acid (AR, 98%) , copper (II) chloride dihydrate (CuCl 2 ・2H 2 O, AR) , and sodium alginate (CP, M v =5000) were purchased from Sinopharm Chemical Regent Co., Ltd. All other chemicals were of analytical grade and used as received, unless otherwise noted.
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8

Synthesis of Metal-Organic Frameworks

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Iron(iii) nitrate nonahydrate (Fe(NO3)3·9H2O, AR), phosphomolybdic acid (H3PMo12O40, PMA, AR), 1,3,5-benzene tricarboxylic acid (H3-BTC) (AR), absolute ethanol (AR), oleic acid (AR), methanol (AR, >99%), lauric acid (AR, 98%), stearic acid (AR, 98%), myristic acid (AR, 98%), palmitic acid (AR, 98%) and methanol (AR, >99%), were purchased from Sinopharm Chemical Regent Co., Ltd. All other chemicals were of analytical grade and used as received, unless otherwise noted.
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9

Synthesis of Functional Materials

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Silver nitrate, ammonium
carbonate, hydrate phosphotungstic acid (H3PW12O40), zirconium(IV) chloride, terephthalic acid, lauric
acid, and methanol (>99%) were purchased from Sinopharm Chemical
Reagent
Co., Ltd. All chemicals were of analytical grade and used without
prior purification.
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