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Ox 40l

Manufactured by R&D Systems
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OX-40L is a cell surface receptor that is expressed on activated T cells. It plays a role in the regulation of T cell-mediated immune responses.

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Lab products found in correlation

Mouse t cell enrichment column, by R&D Systems (1 mentions) Tcrαβ, by Thermo Fisher Scientific (1 mentions) Cona type 4, by Merck Group (1 mentions) Cd40l, by R&D Systems (1 mentions)

2 protocols using ox 40l

1

T Cell Phenotyping by Flow Cytometry

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In some experiments the expression of various molecules on pmel T cells was determined by flow cytometry after activation and analyzed using FlowJo software (Tree Star) at the indicated time points. Antibodies against 4-1BB, OX-40, OX-40L, CD25, CD44, CD62L, CD132, CD127, and GITR used in flow cytometry were purchased from E-biosciences or BD Biosciences. Blocking antibodies to CD28 and GITR were purchased from BD Pharmingen, OX-40L from R&D systems and 4-1BBL (19H3) from Dr. R.S. Mittler.
Joseph A.M., Srivastava R., Zabaleta J, & Davila E. (2016). Cross-talk between 4-1BB and TLR1–TLR2 signaling in CD8+ T cells regulate TLR2’s costimulatory effects. Cancer immunology research, 4(8), 708-716.
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2

T-cell Activation and Cytokine Profiling

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ConA (type IV) was purchased from Sigma (St. Louis, MO, USA). Mouse T-cell enrichment columns and neutralizing antibodies against B7.1, B7.2, OX40L and CD40L were obtained from R&D Systems (Minneapolis, MN, USA). Purified antibodies against mouse CD3, CD28 and cytometric Bead Array for mouse IFN-γ were purchased from BD Biosciences. Fluorochrome-conjugated antibodies against mouse CD3, CD4, CD8, CD19, CD44, CD45.1, CD45.2, B220, NK1.1, TCRαβ and CXCR3 were obtained from eBioscience (San Diego, CA, USA). ALT Detection Kits were purchased from NanJing JianCheng Biochemical Institute (Nan Jing, Jiang Su, China).
Zhao X., Sun G., Sun X., Tian D., Liu K., Liu T., Cong M., Xu H., Li X., Shi W., Tian Y., Yao J., Guo H, & Zhang D. (2016). A novel differentiation pathway from CD4+ T cells to CD4− T cells for maintaining immune system homeostasis. Cell Death & Disease, 7(4), e2193-.
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