α cd11b fitc

Manufactured by BioLegend

Sourced in United States

α-CD11b-FITC is a fluorescently labeled antibody that targets the CD11b antigen. CD11b is expressed on the surface of various immune cells, including monocytes, macrophages, and neutrophils. The FITC (Fluorescein Isothiocyanate) fluorescent label allows for the detection and identification of cells expressing CD11b using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

α cd3 pe, by BD (1 mentions) α ifnγ apc, by BD (1 mentions) Mouse igg1, by Southern Biotech (1 mentions) Facs fortessa, by BD (1 mentions) Intracellular fixation and permeabilization buffer, by Thermo Fisher Scientific (1 mentions) Cellquest, by BD (1 mentions) α cd4 bv711, by BioLegend (1 mentions) α gr1 apc, by BioLegend (1 mentions) α cd3ε 145 2c11, by BioLegend (1 mentions) Anti il 2, by R&D Systems (1 mentions) Anti ly6g pe, by BD (1 mentions) α cd4 apc, by BioLegend (1 mentions) Anti ly6c percp, by Thermo Fisher Scientific (1 mentions) αcd4 pe, by BioLegend (1 mentions) α cd25 pe, by BD (1 mentions) αcd11c apc, by BioLegend (1 mentions)

3 protocols using α cd11b fitc

Comprehensive Immune Profiling by FACS

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Cell suspensions were stained for FACS analysis with the following antibodies: α-CD3-PE (BD Biosciences), α-CD8-BV605 (BD Biosciences), α-CD45-PercP (BD Biosciences), α-CD45-PE (BD Biosciences), α-CD11b-FITC (BioLegend), α-CD11c-FITC (BioLegend), α-CTLA4-PE (BD Biosciences), α-granzyme B-Alexa fluor 647 (BD Biosciences), α-IFNγ-APC (BD Biosciences), α-KI67-BV421 (BD Biosciences), α-TIM3-PE (eBioscience), α-TIGIT-PE (BD Biosciences), α- TNFα-PE (BD Biosciences), α-IL17r-PE (BD Biosciences), and the corresponding isotype control (mouse IgG1, Southern Biotech). The live/dead fixable near-infrared dye (Invitrogen) was used to exclude dead cells. Intracellular fixation and permeabilization buffers from eBioscience were used for cytokine staining.
Acquisition was performed on FACS BD Fortessa and the dedicated software CellQuest (BD Biosciences). Data was analyzed with FlowJo 7.5.5 software (TreeStar Inc).

D’Amico L., Menzel U., Prummer M., Müller P., Buchi M., Kashyap A., Haessler U., Yermanos A., Gébleux R., Briendl M., Hell T., Wolter F.I., Beerli R.R., Truxova I., Radek Š., Vlajnic T., Grawunder U., Reddy S, & Zippelius A. (2019). A novel anti-HER2 anthracycline-based antibody-drug conjugate induces adaptive anti-tumor immunity and potentiates PD-1 blockade in breast cancer. Journal for Immunotherapy of Cancer, 7, 16.

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Evaluating (R)-DI-87 Therapy in Mice

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To assess the therapeutic value and safety of (R)-DI-87, cohorts of female C57BL/6 mice were treated with (R)-DI-87 (75 mg/kg) or vehicle (40% Captisol) via oral gavage in 12-hour intervals over the course of 16 days. On day 16, 100 μl peripheral blood was collected in lithium heparin-coated tubes via retro-orbital bleeding using heparin-coated capillary tubes. Subsequently, blood samples were incubated with 5 ml of ACK lysis buffer at room temperature for 5 min, quenched with 5 ml of FACS buffer (5% FBS in PBS), and centrifuged at 4°C (4 min). This process was repeated and cells were subsequently stained with the following fluorochrome-conjugated anti-mouse antibodies diluted 1:100 in 100 μl of FACS buffer for 20 min at 4°C: α-B220-PerCP/Cy5.5 (103236, BioLegend), α-CD4-BV711 (100550, BioLegend), α-CD8α-PE (100708, BioLegend), α-CD11c-PE/Dazzle594 (117347, BioLegend), α-CD11b-FITC (101206, BioLegend), α-GR1-APC (108412, BioLegend); α-CD16/32 (FC block; 101319; BioLegend). Following incubation, cells were centrifuged and washed twice using FACS buffer. Cells were resuspended in FACS buffer and analyzed using a BD LSRII flow cytometer and the FlowJo software package. During the (R)-DI-87 or vehicle treatment procedure, mice were regularly monitored and weighed to assess the overall health status.

Winstel V., Abt E.R., Le T.M, & Radu C.G. (2023). Targeting host deoxycytidine kinase mitigates Staphylococcus aureus abscess formation. bioRxiv.

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Multiparameter Flow Cytometry Protocol

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Anti-CD11b-PacBlu, αCD11b-FITC, αCD4-PE, αCD4-APC, αCD11c-APC, αCD11c-PE, αTCRvα2-PE, and αCD3ε(145-2C11) were purchased from BioLegend (San Diego, CA, USA). Anti-Ly6C-PerCP and αFoxP3-APC were products of eBioscience (San Jose, CA, USA). Anti-Ly6G-PE, αNK1.1-PE, αCD8α-PacBlu, αCD25-PE, and αCD103-Alexa Fluor 647 were products from BD Biosciences (San Jose, CA, USA). Flt3L-Fc fusion protein was purchased from BioXCell (West Lebanon, NH, USA). Anti-IL-2 was purchased from R&D Systems (Minneapolis, MN, USA). Fc-GITR-L fusion protein was produced as described previously (9 (link)).

Liao G., O’Keeffe M.S., Wang G., van Driel B., de Waal Malefyt R., Reinecker H.C., Herzog R.W, & Terhorst C. (2014). Glucocorticoid-Induced TNF Receptor Family-Related Protein Ligand is Requisite for Optimal Functioning of Regulatory CD4+ T Cells. Frontiers in Immunology, 5, 35.

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