Fitc labeled anti cd16

Freeze-dried BCG (Chengdu Institute of Biological Products, Chengdu, China) was reconsitituted in a 0.9% sodium chloride solution to a concentration of 1 mg/mL prior to use. Sonicatd M.tb protein antigen (TB-Ag) was generously provided by Prof. Baiqing Li (Department of Immunology, Bangbu Medical College, Anhui Key Laboratory of Infection and Immunity, Bangbu, China). The following mAbs were used for phenotypic and intracellular cytokine analysis: phycoerythrin (PE)-labeled anti-CD3, anti-CD14, anti-CD56, anti-CD45RA, anti-IL-17, fluorescein isothiocyanate (FITC)-labeled anti-CD3, anti-CD45RA, anti-CD45RO, anti-granzyme B, Phycoerythrin-Cy7 (PE-cy7)-labeled anti-CD69, anti-CD56, allophycocyanin (APC)-labeled anti-CD3, anti-IFN-γ anti-IL-22 were obtained from BD Pharmingen (San Jose, CA, USA). FITC-labeled anti-CD16 was purchased from Biolegend (CA, USA). PE-labeled anti-NKG2D were obtained from R&D Systems (MN, USA), respectively.