Anti erk antibody

Manufactured by Cell Signaling Technology

Sourced in United States, United Kingdom

The Anti-ERK antibody is a primary antibody that recognizes extracellular signal-regulated kinase (ERK), a family of serine/threonine protein kinases involved in the regulation of various cellular processes. This antibody can be used to detect and analyze the expression and activation of ERK proteins in various experimental systems.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

Anti–p-ERK primary antibody Anti‐phosphorylated ERK antibody Anti-p42/44-phospho-ERK antibody Anti-phosphor-ERK antibody (clone D13.14.4E, Anti-ERK primary antibody Rabbit anti-phospho-ERK polyclonal antibody Anti-ERK antibody (4695) Rabbit anti-phosphorylated ERK antibody Anti-p-ERK antibody Anti-p-ERK monoclonal antibody

40 protocols using anti erk antibody

Western Blot Analysis of Protein Phosphorylation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The amount of protein and the extent of phosphorylation were evaluated with western blotting for both in vivo and in vitro experiments. Antibodies used in the assays included anti-α-SMA antibody (Cat. 100M4795, Sigma), anti-CYBG antibody (Cat. ab57713, Abcam), rabbit monoclonal anti-NF-kB antibody (Cat. 4764, Cell Signaling), anti-p38 antibody (Cat. 9212S, Cell Signaling), anti-P-p38 antibody (Cat. 4511S, Cell Signaling), anti-TGF-β RI antibody (Cat. 2310427, Millipore), anti-TGF-β RII antibody (Cat. 11888, Cell Signaling), anti-ERK antibody (Cat. 4695, Cell Signaling), anti-TGF-β antibody (Cat. #3711 Cell Signaling), anti-P-ERK antibody (Cat. 4370, Cell Signaling), goat anti-rabbit HRP IgG monoclonal antibody (Cat. MR-R100, Shanghai Mingrui Biological Technology Co., Ltd.). The optical densities of the bands were measured and calculated using a gel image analysis system (FR-200, Shanghai Furi Science & Technology Co., Ltd.)

Xu Z.J., Shu S., Li Z.J., Liu Y.M., Zhang R.Y, & Zhang Y. (2017). Liuwei Dihuang pill treats diabetic nephropathy in rats by inhibiting of TGF-β/SMADS, MAPK, and NF-kB and upregulating expression of cytoglobin in renal tissues. Medicine, 96(3), e5879.

+ Open protocol

+ Expand

Antibody Production and Protein Inhibitors

Check if the same lab product or an alternative is used in the 5 most similar protocols

AVM (containing 93% avermectin B1a and 7% avermectin B1b) was obtained from the ZND Bio-technology Co., Ltd. (Beijing, China), and the anti-AVM antibody was a gift from Professor Shen in CAU (Beijing, China). The procedures for preparing the anti-AVM antibody were as follows. First, the structure of AVM was modified by succinylation. Then, the 4″-O-succinyl-AVM was conjugated to bovine serum albumin (BSA). This immunizing antigen was then injected into rabbits, and the polyclonal antibody was acquired [56 (link)].
Lapatinib (EGFR phosphorylation inhibitor) was purchased from Selleck (Houston, TX). Wortmanin (phosphoinositide-3-kinase (PI3K) inhibitor) and U0126 (mitogen-activated protein kinase kinase (MEK) inhibitor) were purchased from Kinasechem (UK). The Relish inhibitor pyrrolidinedithiocarbamic acid (PDTC) was purchased from Sigma-Aldrich (St Louis, MO). The mouse monoclonal anti-P-gp antibody (C219) was purchased from Calbiochem (Darmstadt, Germany). Anti-p-AKT antibody, anti-p-ERK antibody, anti-AKT antibody and anti-ERK antibody were purchased from Cell Signaling Technology (Boston, MA). Anti-EGFR antibody was purchased from Santa Cruz Biotechnology (Dallas, TX). Anti-p-Tyr antibody was purchased from BD Biosciences (Franklin Lakes, NJ). Anti-Relish antibody was purchased from the Developmental Studies Hybridoma Bank (University of Iowa, Iowa City, IA).

Chen L.P., Wang P., Sun Y.J, & Wu Y.J. (2016). Direct interaction of avermectin with epidermal growth factor receptor mediates the penetration resistance in Drosophila larvae. Open Biology, 6(4), 150231.

+ Open protocol

+ Expand

Osteoblast Differentiation Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

Naringin, β-glycerophosphate, L-ascorbic acid-2-phosphate, dimethyl Sulfoxide (DMSO), 1,25-dihydroxyvitamin D3, and Alizarin Red S were purchased from Sigma (St. Louis, MO, USA). Calcium Colorimetric Assay Kit and Alkaline Phosphatase Activity Colorimetric Assay Kit were obtained from Biovision, Inc. The anti-ERK antibody and anti-pERK antibody were purchased from Cell Signaling (Boston, MA, USA). U0126 was from Beyotime (Shanghai, China). All other chemicals were purchased from sigma (St, Louis, MO, eearch Ethical Committee of the USA).

Wang H., Li C., Li J., Zhu Y., Jia Y., Zhang Y., Zhang X., Li W., Cui L., Li W, & Liu Y. (2017). Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells. Iranian Journal of Basic Medical Sciences, 20(4), 408-414.

+ Open protocol

+ Expand

Protein Expression Analysis in HK-2 Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins were obtained from HK-2 cell lysates and animal tissue, using RIPA buffer (Thermo, USA) containing Complete, Mini Protease Inhibitor Cocktail (Roche, USA) and Halt Phosphatase Inhibitor Cocktail (Thermo, USA). To examine the expression of proteins, separated protein in SDS/PAGE (gradient gels) were transferred onto polyvinylidene difluoride membranes (PVDF) (Bio-rad, USA), and then incubated with 5% BSA (sigma) for blocking. The membranes were incubated with anti-GAPDH antibody (Santacruz, USA), anti-type 1 collagen antibody (abcam, UK), anti-fibronectin antibody (abcam), anti-α-SMA antibody, anti-Vimentin antibody, anti-Lin28a antibody, anti-phospho-ERK antibody, anti-ERK antibody, anti-phospho-JNK antibody, anti-JNK antibody, anti-phospho-p38 antibody, anti-p38 antibody, anti-phospho-smad3 antibody and anti-smad3 antibody (Cell signaling, USA). After incubating with HRP-linked antibody (Cell signaling), the protein expression on blots was detected by ChemiDoc^TMXRS+ (Bio-rad) and the bands were quantified using the Image Lab^TM Software (Bio-rad).

Jung G.S., Hwang Y.J., Choi J.H, & Lee K.M. (2020). Lin28a attenuates TGF-β-induced renal fibrosis. BMB Reports, 53(11), 594-599.

+ Open protocol

+ Expand

Western Blot and Immunofluorescence Protocols

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

WB experiments were performed as previously reported [69 (link),93 (link)] using the following antibodies: anti-β-Actin antibody (4967; Cell Signaling Technology, Danvers, Massachusetts), anti-NICD antibody (ab83232; Abcam, Cambridge, UK), anti-Myc antibody (06–549; Millipore), anti-Flag antibody (F7425; Sigma-Aldrich), anti-DLL4 antibody (ab7280; Abcam), anti-JAG1 antibody (ab7771; Abcam), anti-Notch1 antibody (3608; Cell Signaling Technology), anti-Erk antibody (9102; Cell Signaling Technology), anti-p-Erk antibody (9101; Cell Signaling Technology), anti-p-AKT antibody (4060; Cell Signaling Technology), and anti-AKT antibody (9272; Cell Signaling Technology). Quantification of protein level using gray analysis (Gel-Pro analyzer). Immunofluorescence experiments were performed as previously reported [94 (link)] using the following antibodies: anti-Notch1 antibody (3608; Cell Signaling Technology), anti-Notch1 antibody (3447; Cell Signaling Technology), anti-DLL4 antibody (ab7280; Abcam), anti-JAG1 antibody (ab7771; Abcam), anti-LAMP1 antibody (15665; Cell Signaling Technology), anti-EEA1 antibody (610457; BD Biosciences, Franklin Lakes, New Jersey), anti-APPL1 antibody (3858; Cell Signaling Technology), anti-AKT antibody (2920; Cell Signaling Technology), anti-AKT antibody (9272; Cell Signaling Technology), and anti-PIK3CA antibody (ab40776; Abcam).

Heng J., Lv P., Zhang Y., Cheng X., Wang L., Ma D, & Liu F. (2020). Rab5c-mediated endocytic trafficking regulates hematopoietic stem and progenitor cell development via Notch and AKT signaling. PLoS Biology, 18(4), e3000696.

+ Open protocol

+ Expand

MEK/ERK Pathway Immunoblotting

Check if the same lab product or an alternative is used in the 5 most similar protocols

4× SDS loading buffer was added to the reaction aliquots and immunoblotting was performed using standard techniques onto LF PVDF membrane. Primary anti-MEK antibody (1:4000; Cell Signaling Technology, 8727S), anti-dpERK antibody (1:4000; Cell Signaling Technology, 4370S), and anti-ERK antibody (1:4000; Cell Signaling Technology, 4695S) were used to track the formation of total MEK, dpERK, and ERK respectively. Alexa Fluor conjugates (1:2000; Invitrogen) were used as secondary antibodies and membranes were imaged using a Biorad ChemiDoc MP Imaging System.

Goyal Y., Jindal G.A., Pelliccia J.L., Yamaya K., Yeung E., Futran A.S., Burdine R.D., Schüpbach T, & Shvartsman S.Y. (2017). Divergent effects of activating mutations on developmental Ras signaling. Nature genetics, 49(3), 465-469.

+ Open protocol

+ Expand

Analyzing Cell Signaling Pathways

Check if the same lab product or an alternative is used in the 5 most similar protocols

Geneticin (G418) and Lipofectamine 2000 were purchased from Invitrogen/Thermo Fisher Scientific (Waltham, MA, United States). Antibodies: The β_1/2-arrestin antibodies, hemagglutinin (HA) antibody, Anti-p-ERK antibody and Anti-ERK antibody were obtained from Cell Signaling Technology (Danvers, MA, United States). The rabbit Anti-APJ Receptor antibody was obtained Abcam (Cambridge, United Kingdom). The β-actin antibody and secondary antibodies were purchased from ZSGB Bio (Beijing, China).

Jiang Y., Yan M., Wang C., Wang Q., Chen X., Zhang R., Wan L., Ji B., Dong B., Wang H, & Chen J. (2021). The Effects of Apelin and Elabela Ligands on Apelin Receptor Distinct Signaling Profiles. Frontiers in Pharmacology, 12, 630548.

+ Open protocol

+ Expand

Detecting Nitrotyrosine Protein Modifications

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

NK cells were treated with SNAP as described above. 20 μg of anti-nitrotyrosine beads (Millipore 16310) were added to 50 μg of protein lysate and incubated overnight at 4°C. The beads were washed with cold PBS and re-suspended in 2X Laemelli buffer and boiled. The sample was loaded on a SDS gel for protein expression by immunoblot analysis. The membrane was probed with an anti-Erk antibody (Cell Signaling) (28 (link)).

Stiff A., Trikha P., Mundy-Bosse B., McMichael E., Mace T.A., Benner B., Kendra K., Campbell A., Gautam S., Abood D., Landi I., Hsu V., Duggan M., Wesolowski R., Old M., Howard J.H., Yu L., Stasik N., Olencki T., Muthusamy N., Tridandapani S., Byrd J.C., Caligiuri M, & Carson W.E. (2018). Nitric Oxide Production by Myeloid Derived Suppressor Cells Plays a Role in Impairing Fc Receptor-Mediated Natural Killer Cell Function.. Clinical cancer research : an official journal of the American Association for Cancer Research, 24(8), 1891-1904.

+ Open protocol

+ Expand

Western Blot Analysis of Postmortem Brain Lysates

Check if the same lab product or an alternative is used in the 5 most similar protocols

Postmortem brain lysates were prepared from slices of individual tissues, extracts were resolved by sodium dodecylsulfate-polyacrylamide gel electrophoresis followed by Western blotting as previously described [33] . The following antibodies were employed: a mouse monoclonal anti-LAMP2 (H4B4) (1:2000, Santa Cruz Biotechnology, sc18822), a mouse monoclonal anti-LAMP1 (H4A3) (1:2000, Santa Cruz Biotechnology, sc20011), a rabbit monoclonal anti-ACTIN (1:400, Sigma-Aldrich, A2066/030M4844), a monoclonal anti-α-Tubulin (clone DM1A, 1:10,000, Sigma-Aldrich T6199), a rabbit polyclonal Anti-Phospho-AKT473 antibody (1:500, Cell Signaling, 9271), a rabbit polyclonal Anti-AKT antibody (1:500, Cell Signaling, 9272), a rabbit polyclonal Anti-Phospho-ERK antibody (1:500, Cell Signaling, 4370) or a rabbit polyclonal anti-ERK antibody (1:500, Cell Signaling, 4695). The final detection was performed as previously described [33] .

Fernández B., Ferrer I., Gil F, & Hilfiker S. (2017). Biomonitorization of iron accumulation in the substantia nigra from Lewy body disease patients. Toxicology Reports, 4, 188-193.

+ Open protocol

+ Expand

Quantitative Analysis of Clusterin Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

The lysates from cultured cells or cryosections of frozen tissues were prepared and subject to SDS‐PAGE as described previously.²² The primary antibodies used in this study were anti‐CLU antibody (Santa Cruz, #sc‐5289, Dallas, TX, USA), anti‐phosphorylated ERK antibody (Thr²⁰²/Tyr²⁰⁴, Cell Signaling Technology, #4370), anti‐ERK antibody (Cell Signaling Technology, #9102), and anti‐GAPDH antibody (Santa Cruz, #sc‐32,233). Detection was performed with ECL Western Blotting Detection Reagents (Amersham Biosciences, Piscataway, NJ, USA). The signal intensity of CLU or GAPDH in each cell line was quantified using ImageJ. Then, the relative expression of CLU was calculated by dividing the quantified value of CLU by that of GAPDH. CLU was considered to show positive expression when the relative expression was >0.01. In addition, induced expression was considered to have occurred when the fold‐induction, calculated by dividing the relative expression of CLU after the PD0325901 treatment by that before the treatment, was >2.

Amada K., Hijiya N., Ikarimoto S., Yanagihara K., Hanada T., Hidano S., Kurogi S., Tsukamoto Y., Nakada C., Kinoshita K., Hirashita Y., Uchida T., Shin T., Yada K., Hirashita T., Kobayashi T., Murakami K., Inomata M., Shirao K., Aoki M., Takekawa M, & Moriyama M. (2023). Involvement of clusterin expression in the refractory response of pancreatic cancer cells to a MEK inhibitor. Cancer Science, 114(5), 2189-2202.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!