Azm198

BSH was purchased from Carbosynth Ltd (Compton, UK). HOCl was a commercial chlorine bleach product sold by Pental (Melbourne, Australia).Hank's balanced salt solution (HBSS) and phosphate buffered saline (PBS for cell culture, glucose oxidase (GO) from Aspergillus niger (≥100,000 U/g), N‐ethylmaleimide (NEM), 1,4‐dithiothreitol (DTT), taurine, 3,3′,5,5′‐tetramethylbenzidine (TMB), diphenylene iodonium chloride (DPI), sodium azide, bovine liver catalase, lysostaphin from Staphylococcus staphylolyticus, chloramphenicol and D‐(+)‐xylose were purchased from Sigma (Merck, Darmstadt, Germany). Roswell Park Memorial Institute 1640 medium (RPMI,Gibco), Lysogeny broth (LB; Miller's) and tryptic soy broth (TSB) powder were from Thermo Fisher (Waltham, MA, USA), and saponin was from Fluka (Buchs, Switzerland). MPO, from human neutrophils, was supplied by PLANTA (Vienna, Austria) and had a purity index (A₄₃₀/A₂₈₀) of at least 0.82. Dextran from Leucononstoc mesenteroides (average molecular weight: 150,000 Da; Sigma) and Ficoll‐Paque (GE Healthcare, Uppsala, Sweden, and Freiburg, Germany) were used for neutrophil isolation. The specific MPO inhibitors TX1 and AZM198, 2‐thioxanthine molecules,³³ were provided by AstraZeneca (Mölndal, Sweden).