Tissue culture medium tcm 199

Tissue Culture Medium TCM-199, gentamycin, and kanamycin were purchased from Life Technologies Europe (Ghent, Belgium). Phosphate Buffered Saline (PBS), 4-well and 35 mm culture dishes were purchased from Gibco^TM Thermo Fisher Scientific (Waltham, MA, USA). Percoll was purchased from GE Healthcare Biosciences (Uppsala, Sweden) and paraffin oil for tissue culture was obtained from SAGE In Vitro Fertilization (Cooper Surgical Company, Trumbull, CT, USA). Tween80 (Uniqema Americas LCC) was purchased from Cospheric LCC (Goleta, CA, USA). All other chemicals used for the preparation of the culture media were purchased from Sigma-Aldrich (Diegem, Belgium) unless stated otherwise. All media were filtered using 0.2 µm filters from Pall Corporation (Ann Arbor, MI, USA).