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Goat anti rabbit igg h l cross adsorbed secondary antibody

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Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody is a lab equipment product that is used to detect and visualize rabbit primary antibodies in immunoassays. It is a polyclonal antibody raised in goats and has been cross-adsorbed to remove non-specific reactivity.

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Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (4 mentions) Alexa fluor 568, by Thermo Fisher Scientific (4 mentions) Normal goat serum (ngs), by Merck Group (2 mentions) Alexa fluor 594, by Thermo Fisher Scientific (2 mentions) Stattic, by Selleck Chemicals (1 mentions) Ab45039, by Abcam (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Alexa fluor 633 conjugated, by Thermo Fisher Scientific (1 mentions) Metaxpress, by Molecular Devices (1 mentions) Transcription factor buffer set kit, by BD (1 mentions) Imagexpress confocal, by Molecular Devices (1 mentions) Calnexin, by Proteintech (1 mentions) Bz x700, by Keyence (1 mentions) Goat anti mouse igg h l cross adsorbed secondary antibody, by Thermo Fisher Scientific (1 mentions) γ h2ax 2577, by Cell Signaling Technology (1 mentions) Anti flag 2368, by Cell Signaling Technology (1 mentions) Goat anti mouse igg h l secondary antibody, by Thermo Fisher Scientific (1 mentions) Gfp sc 9996, by Santa Cruz Biotechnology (1 mentions) Swine anti rabbit immunoglobulins hrp, by Agilent Technologies (1 mentions) Fitc anti human mouse granzyme b antibody, by BioLegend (1 mentions)

12 protocols using goat anti rabbit igg h l cross adsorbed secondary antibody

1

Ferroptosis Regulation in Cancer Cells

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Traditional Chinese Medicine Library (cat. no. L8300), PPVI (cat. no. S9302), β-lapachone (cat. no. S7261), 3-methyladenine (3-MA, cat. no. S2767) inhibitors and Ac-DEVD-CHO (cat. no. S7901) were purchased from Selleck Chemicals; Stattic (cat. no. HY-13818) and ferrostatin-1 (Fer-1, cat. no. HY-100579) were purchased from MedChem Express. GPX4 (cat. no. ab125066), signal transducer and activator of transcription 3 (STAT3; cat. no. ab68153), phosphorylated (p-)STAT3 (pY705; cat. no. ab76315), N-cadherin (cat. no. ab18203), Vimentin (cat. no. ab92547), E-cadherin (cat. no. ab40772) and goat anti-rabbit IgG H&L (HRP; cat. no. ab6721) antibodies were purchased from Abcam. The GAPDH (2118S) antibody was purchased from Cell Signaling Technology, Inc. The goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody and cyanine3 (A-10520) were purchased from Invitrogen; Thermo Fisher Scientific, Inc.
Huang Q., Li J., Ma M., Lv M., Hu R., Sun J., Zhong X., Sun X., Feng W., Ma W., Zhang W., Zhan B., Han Z, & Zhou X. (2023). High-throughput screening identification of a small-molecule compound that induces ferroptosis and attenuates the invasion and migration of hepatocellular carcinoma cells by targeting the STAT3/GPX4 axis. International Journal of Oncology, 62(3), 42.
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2

CUBIC-Based Tissue Clearing and Immunostaining

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A clear, unobstructed brain imaging cocktail (CUBIC)-based protocol was utilized in clearing the tissue slices (7 (link)). Following clearing tissue slices were washed and immersed in a primary antibody solution consisting of 0.1M PB containing 0.1% TritonX-100 (Sigma), 1% normal goat serum (Sigma), and a 1:250 dilution of ASCT2 (D7C12) Rabbit mAb (8057S, Cell Signaling) for 3 days at 4°C with orbital shaking. After the primary incubation, the tissue was washed and transferred to a secondary antibody solution containing 0.1% TritonX-100 (Sigma), 1% normal goat serum (Sigma), and Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (A-11008, Invitrogen) at a dilution of 1:400 for 3 days at 4oC with orbital shaking. When the secondary incubation was completed, the tissue was washed once again, followed by immersion in EasyIndex (LifeCanvas Technologies) for 1 hour at 37°C with a rocking motion. All washes were performed three times for 2 hours in 0.1M PB at room temperature on a rocking platform.
Chen P., Scarpelli M.L., Healey D.R., Mehta S, & Quarles C.C. (2023). MRI and amino acid PET detection of whole-brain tumor burden. Frontiers in Oncology, 13, 1248249.
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3

CUBIC-Based Tissue Clearing and Immunostaining

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A clear, unobstructed brain imaging cocktail (CUBIC)-based protocol was utilized in clearing the tissue slices (7 (link)). Following clearing tissue slices were washed and immersed in a primary antibody solution consisting of 0.1M PB containing 0.1% TritonX-100 (Sigma), 1% normal goat serum (Sigma), and a 1:250 dilution of ASCT2 (D7C12) Rabbit mAb (8057S, Cell Signaling) for 3 days at 4°C with orbital shaking. After the primary incubation, the tissue was washed and transferred to a secondary antibody solution containing 0.1% TritonX-100 (Sigma), 1% normal goat serum (Sigma), and Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (A-11008, Invitrogen) at a dilution of 1:400 for 3 days at 4oC with orbital shaking. When the secondary incubation was completed, the tissue was washed once again, followed by immersion in EasyIndex (LifeCanvas Technologies) for 1 hour at 37°C with a rocking motion. All washes were performed three times for 2 hours in 0.1M PB at room temperature on a rocking platform.
Chen P., Scarpelli M.L., Healey D.R., Mehta S, & Quarles C.C. (2023). MRI and amino acid PET detection of whole-brain tumor burden. Frontiers in Oncology, 13, 1248249.
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4

Immunocytochemistry of RANKL expression

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Cells in cell culture suspension and urine were collected through centrifugation, and PBS was used to resuspend the sediment. Then, the cells were cytospun onto slides, and fixed using 4% paraformaldehyde for 0.5 hours at room temperature. Thereafter, they were incubated with a primary body, RANKL (ab45039, 1:100, ABCAM, UK), at 4 °C and left overnight. Goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (A11008, 1:500, Invitrogen, USA) was used as the secondary antibody at room temperature for 1 hour, followed by incubation with 5 ug/mL 4',6-diamidino-2-phenylindole (DAPI) (D8417, Sigma, USA) at room temperature for 5 minutes.
Feng Q., Wang D., Feng J., Guo P, & Geng C. (2020). Denosumab inhibits MCF-7 cell line-induced spontaneous osteoclastogenesis via the RANKL/MALAT1/miR-124 axis. Translational Cancer Research, 9(4), 2482-2491.
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5

Quantifying Epigenetic Markers in Immune Cells

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FACS-sorted WT and ThPOK−/− BM progenitors were plated on PolyD lysine coated 96 well plates. Intracellular staining was performed using a Transcription Factor Buffer Set kit (BD Biosciences, Cat: 562574). Primary antibody staining was carried out with 5ug/ml of EZH2 Rabbit polyclonal antibody (Cell signaling: Cat: 5246S) and mouse monoclonal Histone H3K27me3 antibody (Active motif, Cat: 61017), followed by Alexa Fluor™ 633 conjugated Goat anti-Rabbit IgG (H+L) cross-adsorbed secondary antibody and Alexa Fluor 546 conjugated Goat anti-Mouse IgG (H+L) cross-adsorbed secondary antibody (Invitrogen), to measure EZH2 protein and histone H3K27Me3 in the same cells. Finally, cells were stained with DAPI for visualization of the nucleus. In each well of a 96 well plate, eight separate image fields in each wavelength were captured at 20X with 5 z-steps separated by 5um with an automated microscope (ImageXpress Confocal; Molecular Devices, Sunnyvale, CA) driven by MetaXpress software. Two-dimensional projection images were analyzed utilizing the Multi-Wavelength Scoring module (MetaXpress, Molecular Devices) to segment the images for cell morphology parameters and intensity parameters in each channel.
Schnabel E.L, & Jones A.L. (2001). Isolation and Characterization of Five Erwinia amylovora Bacteriophages and Assessment of Phage Resistance in Strains of Erwinia amylovora. Applied and Environmental Microbiology, 67(1), 59-64.
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6

Immunocytochemistry of SH-SY5Y Cells

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A total of 1 × 10⁴ SH-SY5Y cells were seeded in 24-well dishes lined with PLL-coated Cover Glass 12 mm type (4912-040; IWAKI), and the cells were cultured in DMEM with 10% FBS at 37°C and 5% CO2. Next, 4% Paraformaldehyde Phosphate Buffer Solution (09154-85; Nacalai Tasque) was used for fixation for 10 min at room temperature. Both primary and secondary antibodies were used at 200-fold dilution. As the first antibody, Calnexin (66903; proteintech) was used. As the secondary antibody, Goat Anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 594 (A11012; Invitrogen), was used. Fluorescence images were obtained using a fluorescence microscope (BZ-x700; KEYENCE).
Toshima T., Yagi M., Do Y., Hirai H., Kunisaki Y., Kang D, & Uchiumi T. (2024). Mitochondrial translation failure represses cholesterol gene expression via Pyk2-Gsk3β-Srebp2 axis. Life Science Alliance, 7(7), e202302423.
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7

Osteogenic Differentiation Immunofluorescence

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After osteogenic induction for 14 days, cells cocultured with the prepared materials were fixed with 4% PFA. Then, the cells were treated with 0.1% Triton X-100 and blocked with 5% BSA. Afterward, the cells were treated with antibodies OPN (1:500, anti-mouse, Santa Cruz, USA) and RUNX2 (1:500, anti-rabbit, CST, USA) separately overnight at 4°C. After thoroughly washing with PBS, the cells were stained with goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody (1:2000, Invitrogen, USA) and goat anti-rabbit IgG (H+L) cross-adsorbed secondary antibody (1:500, Invitrogen, USA) for 1 h. Finally, the cells were placed in a fluorescent mounting medium with DAPI for further observation under the fluorescence microscope.
Chen P., Zhang C., He P., Pan S., Zhong W., Wang Y., Xiao Q., Wang X., Yu W., He Z., Gao X, & Song J. (2022). A Biomimetic Smart Nanoplatform as “Inflammation Scavenger” for Regenerative Therapy of Periodontal Tissue. International Journal of Nanomedicine, 17, 5165-5186.
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8

Comprehensive Antibody Inventory for DNA Damage Research

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Anti-FLAG (#2368) and γH2AX (#2577) antibodies were purchased from Cell Signaling Technology, whereas antibodies for BRCA1 (#sc-6954), H1.4 (#sc-393358), RNF8 (#sc-2714620), and GFP (#sc-9996) were from Santa Cruz Biotechnology. Antibodies against α-tubulin (GTX628802) were obtained from GeneTex. Anti-RPA (ab2175), H1.0 (ab11079), H1.2 (ab17677), H1.3 (ab183736), and H1.4 (ab18208) were purchased from Abcam, whereas anti-H1.1 was from Insight Biotechnology (GTX117055). Finally, anti-RPA pS4+pS8 (A300-245A) antibodies were purchased from Bethyl, whereas anti-H3 antibodies were obtained from Sigma-Aldrich (05-928). Goat anti-mouse immunoglobulins/HRP (#P044701-2) and swine anti-rabbit immunoglobulins/HRP (P039901-2) were purchased from Dako. Goat anti-mouse IgG (H + L) secondary antibody, Alexa Fluor 488 (#A10680) and goat anti-rabbit IgG (H + L) cross-adsorbed secondary antibody, and Alexa Fluor 568 (#A11011) were purchased from Thermo Fisher Scientific.
Ozgencil M., Dullovi A., Christiane Higos R.C., Hořejší Z, & Bellelli R. (2023). The linker histone H1–BRCA1 axis is a crucial mediator of replication fork stability. Life Science Alliance, 6(9), e202301933.
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9

Immunofluorescence Analysis of Murine Organs

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Murine organs were fixed in phosphate-buffered 4% formaldehyde and embedded in paraffin. Three to five micrometres thick sections were stained with hematoxylin and eosin.
Immunofluorescence staining on 5-μm paraffin sections using antibodies against cleaved caspase3 (Cell Signaling, #9664), Goat anti-Rabbit IgG (H + L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 568 (ThermoFisher, #A-11011), and FITC anti-human/mouse Granzyme B Antibody (BioLegend, #515403) was performed as described8 (link),51 (link),52 (link).
Shen T., Chen Z., Qiao J., Sun X, & Xiao Q. (2019). Neutralizing monoclonal antibody against Dickkopf2 impairs lung cancer progression via activating NK cells. Cell Death Discovery, 5, 123.
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10

Immunoblotting: Protein Detection and Imaging

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For immunoblotting, proteins were transferred to polyvinylidene fluoride Immobilon-FL or Immobilon-P membranes (Millipore). The transferred proteins were detected by fluorophore- or horseradish peroxidase (HRP)-conjugated to secondary antibodies (Cy5 AffiniPure Goat Anti-Rabbit IgG (H+L), Jackson ImmunoResearch Laboratories) or goat anti-Rabbit IgG (H+L) cross-adsorbed secondary antibody conjugated to HRP (Thermo Fisher Scientific) and analyzed with a Typhoon imager (GE Healthcare) or LAS-4000 mini device (Fujifilm).
Kakimoto-Takeda Y., Kojima R., Shiino H., Shinmyo M., Kurokawa K., Nakano A., Endo T, & Tamura Y. (2022). Dissociation of ERMES clusters plays a key role in attenuating the endoplasmic reticulum stress. iScience, 25(11), 105362.
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