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2 protocols using water in optima grade

1

Fatty Acid and Oxidant Analysis

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Oleic acid (FA 18:1 (Δ9)), cis-vaccenic acid (FA 18:1 (Δ11)), linOleic acid (FA 18:2 (Δ9, Δ12)), γ-linolenic acid (FA 18:3 [Δ6, Δ9, Δ12], GLA), linolenic acid (FA 18:3 (Δ9, Δ12, Δ15), ALA) and 3-chloroperbenzoic acid (mCPBA) were purchased from Sigma-Aldrich (St Louis, MO, USA). Acetonitrile, isopropanol, formic acid, and water in Optima grade were purchased from Fisher Chemical (Fair Lawn, NJ, USA). Nitrogen gas for purging the sample stage enclosure was purchased from Arc3 Gases (Raleigh, NC, USA).
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2

Preparation of Analytical Reagents

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Phosphate buffered saline (PBS) buffer was purchased from Gibco (Grand Island, NY, USA). Acetonitrile, methanol, formic acid, and water in Optima grade were purchased from Fisher Chemical (Fair Lawn, NJ, USA). Nitrogen gas used to purge the sample stage enclosure was purchased from Arc3 Gases (Raleigh, NC, USA).
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