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2 protocols using anti pi3kδ antibody

1

Multiparametric Analysis of PI3K Signaling

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Anti-phosphorylated and pan, AKT, and MAPK antibodies, and PI3Kα/β/γ antibodies were purchased from Cell Signalling Technology (Cambridge, MA, USA). Anti-PI3Kδ antibody was purchased from R&D systems (Oxford, UK). Anti-CD138-PE, anti-CD90-FITC, anti-CD73-PE, anti-CD105-APC antibodies (Cat. 130-098-122, 130095403, 130095182, 130094926) and interleukin-6 (IL-6) were purchased from Miltenyi Biotec (Auburn, CA, USA). Idelalisib, CZC24832, duvelisib were obtained from Selleck Chemicals (Houston, TX, USA). All other reagents were obtained from Sigma-Aldrich (St Louis, MO, USA), unless otherwise indicated.
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2

Characterization of Stem Cell Markers

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Anti-phosphorylated and pan, AKT, and MAPK antibodies, as well as PI3Kγ were purchased from Cell Signalling Technology (Cambridge, MA, USA). Anti-CD34-PE, anti-CD90-FITC, anti-CD73-PE, anti-CD105-APC antibodies as well as SDF-1 were purchased from Miltenyi Biotec (Auburn, CA, USA). Anti-PI3Kδ antibody was purchased from R&D systems (Abingdon, UK). LY294002, CAL101, IPI-145, MK2206 and AZD6224 were obtained from Selleck Chemicals (Tx, USA). All other reagents were obtained from Sigma-Aldrich (St Louis, MO, USA), unless indicated.
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