The interaction between the mycobacteriophage D29 and its host strain was examined by transmission electron microscopy. Infection was performed at an MOI of 1. Samples collected at different stages of infection were negatively stained with 2% uranyl acetate and examined under an FEI Tecnai 12 Biotwin transmission electron microscope (FEI, Netherlands) at an accelerating voltage of 100kV.
Tecnai 12 biotwin transmission
Manufactured by Thermo Fisher Scientific
Sourced in Netherlands
The Tecnai 12 BioTWIN is a transmission electron microscope designed for biological applications. It provides high-resolution imaging and analysis capabilities for a variety of samples. The core function of the Tecnai 12 BioTWIN is to magnify and visualize the internal structure of biological specimens, enabling researchers to study their morphology, ultrastructure, and other characteristics at the nanoscale level.
Automatically generated - may contain errors
3 protocols using tecnai 12 biotwin transmission
1
Visualizing Mycobacteriophage D29 Infection
2
Tick Gut Ultrastructure and Protein Localization
3
Mitochondria-ER Contacts in Human Cells
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Ultrathin sections from human biopsies were processed using Leica Ultracut UCT (Leica, Vienna, Austria) and contrasted with uranyl acetate and lead citrate. Sections were observed with a Tecnai 12 BioTWIN transmission electron microscope (FEI Company, Eindhoven, The Netherlands) at 100 kV. Digital images were acquired with a Veleta camera (Olympus Soft imaging Solutions, GmbH, Münster, Germany) at a primary magnification of 20.500×.
Pictures were acquired as before [6 (link)]. Briefly, 10 random cells were chosen per patient and, for each cell, pictures of all visible mitochondria were taken. In total, 140 cells were analysed, including more than 800 MERCS and 2000 mitochondria. The number of MERCS and mitochondria as well as MERCS length and mitochondria perimeter were obtained using iTEM FEI software (EMSIS GmbH, Muenster, Germany). MERCS were considered as such when the distance between ER and mitochondria was equal or bellow 30 nm. Values presented in Additional file1 : Table S1 represent average values per cell profile per patient. The overall quality of the tissue was very high and representative images are shown. In particular, the structure of mitochondria was very well preserved while the ER structures sometimes appeared dilated.
Pictures were acquired as before [6 (link)]. Briefly, 10 random cells were chosen per patient and, for each cell, pictures of all visible mitochondria were taken. In total, 140 cells were analysed, including more than 800 MERCS and 2000 mitochondria. The number of MERCS and mitochondria as well as MERCS length and mitochondria perimeter were obtained using iTEM FEI software (EMSIS GmbH, Muenster, Germany). MERCS were considered as such when the distance between ER and mitochondria was equal or bellow 30 nm. Values presented in Additional file
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