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Uas lifeact gfp

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UAS-LifeAct.GFP is a genetic construct that expresses the LifeAct-GFP fusion protein. LifeAct is a small peptide that binds to filamentous actin, allowing visualization of the actin cytoskeleton. The GFP (Green Fluorescent Protein) tag enables the visualization of the actin structures in living cells.

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Lab products found in correlation

Tub gal80ts, by BD (1 mentions) Uas lifeact ruby, by BD (1 mentions) Trpml1 tm6b, by BD (1 mentions) Uas dcr2, by BD (1 mentions) Hmlδ gal4, by BD (1 mentions) Cg gal4, by BD (1 mentions) Uas eb1 gfp, by BD (1 mentions) Uas gfp, by BD (1 mentions) Tub gal4, by BD (1 mentions) Arm gfp, by BD (1 mentions) Sqh eyfp golgi, by BD (1 mentions) Uas mcherrycaax, by BD (1 mentions) Uas mcherry nls, by BD (1 mentions) Uas dicer2, by BD (1 mentions)

3 protocols using uas lifeact gfp

1

Drosophila Genetics for Cell Dynamics

Cited 3 times
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All experiments were performed with D. melanogaster strains acquired from Bloomington or Vienna Drosophila Stock Centers unless otherwise indicated: HmlΔ-Gal4 (BDSC:30141), Cg-Gal4 (BDSC:7011), UAS-LifeAct.GFP (BDSC:35544), UAS-LifeAct.Ruby (BDSC:35545), HmlΔ>2xEGFP (BDSC:30140), Ubi::Cad.GFP, Srp>Gal4, UAS-GFP/CyO; Crq>Gal4, UAS-RedStinger (Weavers et al., 2016 (link)), sqh::sqh-3xGFP (Pinheiro et al., 2017 (link)), α-Tub-GFP (Grieder et al., 2000 (link)), DE-Cad.GFP, UAS-H2B.RFP/CyO (Huang et al., 2009 (link)), UAS-trpml-GCaMP.5T/TM6b (Wong et al., 2017 (link)), Hs::FLP; UAS-LifeActRuby/CyO, act::GFP; Act <FRT> Gal4, trpml1/TM6b (BDSC:28992), trpml2/TM6b (BDSC:42230), UAS-trpml (BDSC:27594), UAS-trpml-myc, trpml1/TM6b (BDSC:57372), UAS-TRPML1-HA; trpml1/TM6b (BDSC:57373), TRiP.JF01466 (BDSC:31673), TRiP.GL01524 (BDSC:43543), TRiP.HMS00521 (BDSC:32516), TRiP.HMS00437 (BL32439), UAS-GFP.zipDN (Franke et al., 2005 (link)), UAS-mypt75DF117A (BDSC:24099), Tub::Gal80TS (BDSC:7017), UAS-dcr2 (BDSC:24650), fab1 RNAi (VDRC:27591), and UAS-GCaMP6m (BDSC:42748).
Edwards-Jorquera S.S., Bosveld F., Bellaïche Y.A., Lennon-Duménil A.M, & Glavic Á. (2020). Trpml controls actomyosin contractility and couples migration to phagocytosis in fly macrophages. The Journal of Cell Biology, 219(3), e201905228.
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2

Drosophila Genetics and Imaging Protocol

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All flies used in experiments were reared and maintained at 25°C on cornmeal fly food with dark/light cycle. For most experiments, white pre-pupae (0 h APF) of the desired genotype were collected and staged at 25°C. Following fly lines that are described in FlyBase and were obtained from the Bloomington Drosophila stock center (BDSC), the Vienna Drosophila Resource Center (VDRC), or the Kyoto Drosophila Genetic and Genomic Resources Center (DGGR), unless noted otherwise were used: UAS-EB1-GFP (BDSC 35512), Ecad-mTomato (BDSC 58789), UAS-Tub-EOS (BDSC 51313), Arm-GFP (BDSC 8555), tubGal4 (BDSC 5138), UAS-Lifeact-GFP (BDSC 35544), FRT42DtubGal80 (II), FRT42Dsspey05252 (DGRC 114436), hsFLP, Tub-Gal4 (BDSC 5138), UAS-GFP (BDSC 5430) sqh-EYFP-Golgi (BDSC 7193), His2avD-mRFP (BDSC 23651), Patronin RNAi (VDRC108927), Ubi-beta-TubGFP (DGRC 109604), UAS-palmKate2-K7 (attP2) (III) from Stefan Luschnig (University of Münster, Germany), GFP-Patronin22H2-N (attP40) (III) (Takeda et al., 2018 (link)), ciGal4 (Croker et al., 2006 (link)), and Ubi-p63E-cnn-RFP (Conduit et al., 2010 (link)).
For clonal analysis patroniney05252 FRT42D mutant positively marked clones were generated using FLP-mediated recombination. Clones were induced by heat-shock of prepua (0 h APF) at 37°C for 10 min in a water bath.
Panzade S, & Matis M. (2021). The Microtubule Minus-End Binding Protein Patronin Is Required for the Epithelial Remodeling in the Drosophila Abdomen. Frontiers in Cell and Developmental Biology, 9, 682083.
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3

Imaging Drosophila Cytoskeleton and Lineage

Cited 1 time
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The following Drosophila stocks were used: R10H12-gal4 (Pfeiffer et al., 2008 (link); BDSC 48278), UAS-mCherryCAAX (BDSC 59021), UAS-mCherryNLS (BDSC 38425), enhancer trap lines sr-gal4md710 (Usui et al., 2004 (link), BDSC 2663), UAS-Lifeact.GFP (BDSC 35544), UAS-dar1RNAi (BDCS 31987), dar13010 (BDSC 65269), UAS-GC3Ai (Schott et al., 2017 (link), BDSC 84346), UAS-serpRNAi (BDSC 63556), UAS-vermRNAi (BDSC 57188), UAS-lolalRNAi (BDSC 35722), UAS-Dicer2 (BDSC 24650), UAS-SrDN (gift from Vorbrüggen and Jäckle, 1997 (link)) and G-TRACE line (Evans et al., 2009 (link), BDSC 28280).
Laurichesse Q., Moucaud B., Laddada L., Renaud Y., Jagla K, & Soler C. (2021). Transcriptomic and Genetic Analyses Identify the Krüppel-Like Factor Dar1 as a New Regulator of Tube-Shaped Long Tendon Development. Frontiers in Cell and Developmental Biology, 9, 747563.
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