NMR spectra were acquired on an ECA-600 (JEOL, Tokyo, Japan) using dimethyl sulfoxide (DMSO)-d6 as a solvent. UV spectra were measured on a U-2910 spectrometer (Hitachi, Tokyo, Japan) in MeCN−0.1% (vol vol−1) formic acid aq. (7:1) or MeOH−0.1% (vol vol−1) formic acid aq. (21:4) at a concentration of 50 µM. A mass spectrum was obtained on a micrOTOF-Q II−ESI-Qq-TOF spectrometer (Bruker, Billerica, MA, USA) using ESI-L Low Concentration Tuning Mix (Agilent Technologies, Santa Clara, CA, USA) as the internal standards. The sample was dissolved in MeCN−0.1% (vol vol−1) formic acid aq. (7:1) for the mass analysis. IR spectrum was measured on a FT/IR-4100 type A spectrometer (JASCO, Tokyo, Japan) using the KBr method. A fluorescence spectrum was acquired on a F-2500 spectrometer (Hitachi, Tokyo, Japan) as a solution in MeCN−0.1% (vol vol−1) formic acid aq. (7:1).
U 2910 spectrometer
Manufactured by Hitachi
Sourced in Japan
The U-2910 spectrometer is a compact and versatile laboratory instrument designed for various spectroscopic applications. It is capable of performing accurate measurements in the ultraviolet and visible light spectrum. The core function of the U-2910 is to analyze and quantify the absorption or transmission of light through samples, providing users with essential data for their research and analysis needs.
Automatically generated - may contain errors
Lab products found in correlation
Eca 600, by JEOL (1 mentions)
F 2500 spectrometer, by Hitachi (1 mentions)
Ft ir 4100 type a spectrometer, by Jasco (1 mentions)
Esi l low concentration tuning mix, by Agilent Technologies (1 mentions)
Affinity 1, by Shimadzu (1 mentions)
Advance 600 spectrometer, by Bruker (1 mentions)
Silica gel, by Cytiva (1 mentions)
Sephadex lh 20 gel, by Cytiva (1 mentions)
Mcp 300, by Anton Paar (1 mentions)
Esquire 3000 plus spectrometer, by Bruker (1 mentions)
Prominence system, by Shimadzu (1 mentions)
Maxis q tof mass spectrometer, by Bruker (1 mentions)
C18 column, by YMC (1 mentions)
Avance 700 spectrometer, by Bruker (1 mentions)
1260 hplc system, by YMC (1 mentions)
Prism 6, by GraphPad (1 mentions)
8 protocols using u 2910 spectrometer
1
Comprehensive Analytical Characterization
2
Comprehensive Analytical Characterization
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FT-IR spectrometer (Affinity-1, Shimadzu) was used to measure IR spectra. Optical rotations were measured in a polarimeter (MCP 300, Anton Paar) at 25°C. U-2910 spectrometer (Hitachi) was used to record UV spectra. Advance 600 spectrometer (Bruker) was used to measure 1H NMR (600 MHz) and 13C NMR (150 MHz). Esquire 3000 plus spectrometer (Bruker) was used to measure ESIMS spectra. A micro TOF-QII mass spectrometer (Bruker) was used to record HRESIMS data. Sephadex LH-20 gel (Amersham Pharmacia) and silica gel (100–200 mesh and 200–300 mesh; Qingdao Marine Chemicals) were used in column chromatography. Analytical and preparative HPLC was performed on a Shimadzu Prominence system. Circular Dichroism Spectrometer (V100) was used to measure CD spectra.
3
Quantification of Glycosaminoglycan Content
4
Detailed Analytical Techniques for Compound Characterization
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U-2910 spectrometer (Hitachi) was used to record the UV spectra of compounds 17 –21 . The 1D and 2D NMR spectra of compounds 17 –21 were obtained with a Bruker Avance-700 spectrometer. All of the mass spectra were acquired on a Bruker MaXis Q-TOF mass spectrometer. Semi-preparative HPLC was performed on an Agilent 1260 HPLC system with a C18 column (YMC, 10 mm × 250 mm, 5 μm).
Strains and constructed plasmids utilized are shown in Supporting InformationTables S3‒S4 . LB medium was added with an additional antibiotic at a concentration of 50 μg/mL carbenicillin or 50 μg/mL kanamycin when necessary. Solid CD Medium was used for incubations of A. nidulans A1145 and Aspergillus sp. SCSIO SX7S7. The mutant strains of SCSIO SX7S7 were grown at Solid CD Medium with additional 200 μg/mL hygromycin (Hyg). Analytical or chromatographic-grade chemicals and solvents were used in this study.
Strains and constructed plasmids utilized are shown in Supporting Information
5
Bacterial Growth Assay with Verapamil
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Bacterial growth assay was conducted as described previously [52 (link)]. A single colony of bacteria was inoculated in LB media and cultured at 37 °C. For plate assay, 30 μL of bacterial culture (OD600 = 0.4) was transferred to an NGM plate either or not containing verapamil (100 μM and 400 μM), and cultured at 20 °C. The bacteria were washed off using 1 mL M9 buffer and OD600 was measured every 12 h, with M9 buffer as the blank control. OD was assessed using a Hitachi U-2910 spectrometer with a 10-mm quartz cuvette. Three replicate experiments were conducted, and results were generated using GraphPad Prism 6. Unpaired t-test was used to assess the significance.
6
Bacterial growth assay in OP50 culture
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Bacterial growth assay was conducted as described previously [27 (link)]. Initially, a single colony of OP50 was inoculated in LB media and cultured at 37°C. For each group, 30 μL of bacterial culture (OD600 = 0.4) was dropped to an NGM plate and cultured at 20°C. Bacteria were washed off using 1 mL M9 buffer, and OD600 was measured every 12 h, with M9 buffer as the blank control. OD was assessed using a Hitachi U-2910 spectrometer with a 10 mm quartz cuvette. Three replicate experiments were conducted. An unpaired t-test was used to calculate the P values, and error bars represented SEM.
7
Bacterial Growth Inhibition Assay
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A single colony of bacteria was inoculated in the LB media and cultured at 37 ℃. For plate assay, 30 μL of bacterial culture (OD600 = 0.4) was transferred to an NGM plate either with or without crotamiton or JM03 at a concentration of 400 μM, and cultured at 20 ℃. The bacteria were washed off using 1 mL M9 buffer and OD600 was measured every 12 hr, with M9 buffer as the blank control. OD was assessed using a Hitachi U-2910 spectrometer with a 10 mm quartz cuvette. At least three technical and three biological independent replicates were performed.
8
Bacterial Growth Assay on NGM Plates
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Bacterial growth assays were performed following the method described previously [13 (link)]. OP50 was plated onto solid LB medium and cultured inverted at 37°C overnight. In the next day, a colony was picked and inoculated into liquid LB medium, followed by culturing at 37°C for 16 h. The bacterial culture was diluted in LB medium to OD600 = 0.4, and 30 μL of the dilution was added onto NGM plates at 20°C. The plates were rinsed with M9 buffer at 6 different time points (12 h, 24 h, 36 h, 48 h, 60 h, and 72 h) after incubation, and the OD600 was measured on a Hitachi U-2910 spectrometer. The experiments were repeated three times independently, and the statistical significance of the growth inhibition was assessed by multiple t-tests.
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