Primary normal human dermal fibroblasts (HDMFs; ATCC® PCS-201-010™) were maintained in DMEM medium (GIBCO) containing 20% FBS (Seradigm), 0.2 mM L-Glutamine (Gibco), and 100 U/ml penicillin/streptomycin (Gibco). Murine myoblasts (C2C12; ATCC® CRL-1772) were cultured in DMEM medium + GlutaMAX™ (Gibco) containing 10% FBS (Seradigm) and 100 U/ml penicillin/streptomycin (Gibco). Primary patient-derived CMCs were propagated in Ham’s F12 medium (Gibco) supplemented with 10% FBS (Seradigm), 20 ng/ml Recombinant Human bFGF (PeproTech), 0.2 mM L-Glutamine (Gibco), 0.005 U/ml human Erythropoietin (Sigma), and 100 U/ml penicillin/streptomycin (Gibco). Human HEK293FT cells (Life Technologies) used in lentiviral production were grown in DMEM medium (Gibco) containing 10% FBS (Seradigm) and 0.2 mM L-Glutamine (Gibco). All cell lines were propagated under standard incubation conditions at 37°C with 5% atmospheric CO2 and, further, were enzymatically passaged using TrypLE™ (Life Technologies) when approaching ≈70% confluence.
Human hek293ft cells
Manufactured by Thermo Fisher Scientific
HEK293FT cells are a human embryonic kidney cell line that has been engineered to stably express the SV40 large T antigen. They are commonly used in research for the production of lentiviral and retroviral vectors.
Automatically generated - may contain errors
Lab products found in correlation
Ham s f12 medium, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Human recombinant bfgf, by Thermo Fisher Scientific (1 mentions)
L glutamine, by Thermo Fisher Scientific (1 mentions)
Glutamax, by Thermo Fisher Scientific (1 mentions)
Tryple, by Thermo Fisher Scientific (1 mentions)
Crl 1772, by American Type Culture Collection (1 mentions)
Human erythropoietin, by Merck Group (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
2 protocols using human hek293ft cells
1
Culturing Primary Cells and Cell Lines
2
Cell Line Acquisition and Maintenance
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human HEK293FT cells (female) were obtained from (Life Technologies). Human HeLa cells (female) were obtained from Dr. Stephen Briddon (University of Nottingham) (Rose et al., 2012 (link)). Cells lines were not subsequently authenticated. HeLa and HEK293T cells were transfected and cultured as described in Method Details .
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