For determination of energy expenditure, respiratory exchange ratios, and activity, mice were placed in an Oxymax metabolic chamber system (Comprehensive Laboratory Animal Monitoring System, CLAMS, from Columbus Instruments (Columbus, OH)). Oxygen consumption (V̇O2), carbon dioxide production (V̇CO2), and ambulatory activity were determined for each mouse over 72 hours Only the last two complete light and dark cycles (spanning 48 hours) were used for data analysis. Feces were collected during the metabolic cage studies and shipped to the Mouse Metabolic Phenotyping Core at the University of Texas Southwestern for bomb calorimetry analysis using a Parr 6200 Isoperibol Calorimeter.
6200 isoperibol calorimeter
Manufactured by Parr
Sourced in United States
The 6200 Isoperibol Calorimeter is a laboratory equipment designed to measure the heat of a chemical reaction or physical process. It operates on the principle of isoperibol calorimetry, where the temperature change of a sample is measured in a thermally insulated environment to determine the enthalpy change of the system.
Automatically generated - may contain errors
Lab products found in correlation
Model 1108 oxygen bomb, by Parr (2 mentions)
Oxymax metabolic chamber system, by Columbus Instruments (1 mentions)
Manual pellet press, by Parr (1 mentions)
Kjeltec 2300 analyzer, by Foss (1 mentions)
Humic acid, by Thermo Fisher Scientific (1 mentions)
Lignin, by Merck Group (1 mentions)
Speedvac concentrator, by Thermo Fisher Scientific (1 mentions)
Milliplex map human cytokine chemokine panel, by Merck Group (1 mentions)
Soxtec 2055, by Foss (1 mentions)
Kjeltec 8400, by Foss (1 mentions)
17 protocols using 6200 isoperibol calorimeter
1
Metabolic Profiling of Mice
2
Calorimetric Analysis of Biomass Fuels
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‘Bomb’ calorimetric runs were performed using a Parr 6200 isoperibol calorimeter (Parr Instrument Company, Moline, IL, USA) to determine the calorific values of the CMP and FWMP in accordance with BS EN ISO 18125:2017 [15 ]. The measurements were conducted on the samples, in the form of a pellet, weighing ca. 0.5 g for CMP and ca. 1 g for FWMP. The ‘bomb’ was filled with oxygen up to a pressure of 31 bars and ignited. For each sample, duplicate runs were performed, and the average values are presented.
Microcone Combustion Calorimetry (MCC) measurements were performed using a Fire Testing Technology Ltd. (Gosport, UK) microcone combustion calorimeter according to ASTM D7309. For each run, an accurately weighed sample was firstly heated to about 900 °C at a heating rate of ca. 60 °C/min, in a stream of nitrogen. The thermal degradation products were collected and then mixed with a stream of air prior to entering a combustion chamber maintained at 900 °C. All the tests were run in triplicates, and the average values are reported.
Microcone Combustion Calorimetry (MCC) measurements were performed using a Fire Testing Technology Ltd. (Gosport, UK) microcone combustion calorimeter according to ASTM D7309. For each run, an accurately weighed sample was firstly heated to about 900 °C at a heating rate of ca. 60 °C/min, in a stream of nitrogen. The thermal degradation products were collected and then mixed with a stream of air prior to entering a combustion chamber maintained at 900 °C. All the tests were run in triplicates, and the average values are reported.
3
Comprehensive Biofuel Characterization Protocol
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analysis have been described in detail in previous studies.42 (link),43 (link)
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A Parr 6200 Isoperibol calorimeter
(Parr Instrument Company, Moline, IL) was used to measure the heat
of combustion based on ASTM D480937 and
reported as the higher heating value (HHV).
A Setaflash Series 8 closed cup flash
point analyzer (model 82000-2 U) was used to measure the flash point
of oil samples according to the ASTM D3828 method38 (hot wire ignition).
The free fatty acid contents (FFAs)
and iodine values (IVs) of the pongamia oils were determined using
American Oil Chemist’s Society (AOCS) methods Ca 5a-4039 and Cd 1d-92,40 respectively.
Differential scanning calorimetry
(DSC)
analyses were conducted using a TA Q2000 system (TA Instruments, New
Castle, DE) equipped with an RCS90 temperature control, which permits
operation over the temperature range of −90 to 400 °C.
The DSC measurement method has been described in detail in a previous
publication.41 (link) The cooling scan was analyzed
to determine the crystallization onset temperature (FO) and the crystallization
peak temperature (FP), which reflect the low-temperature quality of
the oils.
An Anton Paar
SVM3000 Stabinger viscometer
(Anton Paar USA Inc., Ashland, VA) was used to measure the viscosity
and density of the pongamia oil samples at temperatures according
to the ASTM D7042 method.3
analysis have been described in detail in previous studies.42 (link),43 (link)
4
Calorimetric Analysis of Mouse Stool
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Stool was obtained from individually-housed male animals of either genotype (EECΔCol or WT – Neurog3fl/fl or Neurog3R/fl) fed regular chow. Mice were placed in wire bottom cages for a 48h period, and the entire stool output collected from the cage. Stool was submitted to the UTSW Mouse Metabolic Phenotyping Core, which uses a Parr 6200 Isoperibol Calorimeter Equipped with a 6510 Parr Water Handling system for calorific tests. Gross heat of sample combustion is reported and normalized per total stool output in a 24h period.
5
Proximate Analysis of Basal Diet and CSE
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The basal diet and CSE were analyzed to assess their proximate composition. The analytical determinations were conducted in triplicate. The dry matter content (DM) of the diet samples was measured using gravimetry, specifically the AOAC technique n. 934.01. The ash content (ASH) was determined using gravimetric analysis following sample incineration using a muffle furnace, according to the AOAC method n. 942.05. The Kjeldahl method (AOAC method n. 978.04) was used to analyze the crude protein (CP), while the ether extract (EE) was determined using Soxhlet extraction (AOAC method n. 920.39). The acid detergent fiber was measured according to the method outlined by Goering and Van Soest [22 ]. The gross energy (MJ/kg DM) of the food samples was determined using a Parr 6200 Isoperibol Calorimeter (Parr Instrument Company, Moline, IL, USA) following the instructions provided by the manufacturer.
6
Fecal Energy Content and Childhood Enteropathy
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In those children who undergo endoscopy at AKUH, fecal calorimetry (6200 Isoperibol Calorimeter; Parr Instrument Company, Moline, IL, USA) will be performed to obtain macronutrient specific determination of fecal energy [35 (link)]. Total protein, fat, and carbohydrate energy content of a single fecal aliquot will be compared against the child’s clinical phenotype (including severity of wasting), fecal and duodenal enteropathogen burden, endoscopic inflammation, and histologic severity.
7
Calorimetric Analysis of Test Meals
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The energy (kcal/g) of each test meal was determined using bomb calorimetry. Each test meal was prepared identical as if it were to be served to a participant. Meals were homogenized using a food-grade commercial blender (Model HBH450, Hamilton Beach Commercial, Glen Allen, VA) on high setting for 1 min and then passed through a 2 mm sieve (Advantech 2.00 mm USA standard testing sieve No. 10, New Berlin, WI). Aliquots were weighed and then lyophilized at −55 °C using a Uni-Trap Model 10–100 (The Virtis Company, Gardiner, NY). Lyophilized samples were ground and passed through a 1 mm screen before being pelleted into duplicate ~1.00 g pellets using a manual pellet press (Parr Instrument Co., Moline, IL). Pellets were placed within a model 1108 oxygen bomb (Parr Instrument Co.) and bombed in a 6200 Isoperibol calorimeter (Parr Instrument Co.). Each test meal was analyzed in duplicate with an accepted CV of less than 2 % between duplicate samples. Calorimetry standard 1.00 g benzoic acid pellets (Parr Instrument Co.) were used to calibrate each bomb before the analysis of test meal pellets.
8
Bomb Calorimetry of Zeolitic Imidazolate Framework
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The bomb calorimetry measurement on ZIF-8 was carried out on the 6200 Isoperibol Calorimeter (Parr Instrument Company, Moline, IL, USA), which is a microprocessor-controlled, isoperibol oxygen bomb calorimeter.
9
Proximate Composition Analysis of Experimental Diets and Fish Tissues
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Proximate composition analyses of the experimental diets, fish body, and muscle and liver tissue were performed according to standard procedures (AOAC, USA, 1995) [103 ]. In brief, moisture content was assessed by oven-drying at 105 °C for 24 h. Ash content was assessed by muffle furnace incineration at 550 °C for 12 h. Crude protein content (N × 6.25) was assessed by the Kjeldahl procedure (Kjeltec 2300 Analyzer, Foss Tecator, Höganäs, Sweden). A Soxtec System HT (Soxtec System HT6, Tecator, Sweden) was used for lipid quantification by the ether extraction method. Gross energy content was assessed by using a 6200 Isoperibol Calorimeter (Parr Instrument Company, Moline, IL, USA) for the bomb calorimetry procedure.
10
Biomass Calorific Value Determination
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The gross calorific value was determined by a 6200 Isoperibol calorimeter (Parr Instruments), calibrated by combustion of certified benzoic acid. The dried biomass was analysed as given in the Standard Methods (DIN 51900-1:2000) [53 ]. The sample was placed in the sampler holder of the bomb. The bomb was assembled, filled with oxygen for 30 s at a pressure of 400 psi and placed in the calorimeter. The sample was burned under controlled conditions for 15 min.
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