SMMC-7721 cells (1 × 104) (or HepG2, Huh7) were plated in a 96-well plate. Cells were cultured with RPMI-1640 containing 10% FBS and 0, 3.15, 6.3, 12.5, 25, or 50 mg/ml of S. barbata for 48 h. Inhibitory rate of cell proliferation was then identified by Cell Counting 8 Kit (CCK8; Beyotime, Shanghai, China) following the manufacturer’s protocol. Inhibitory rate = (1 − mean OD value of experimental group/mean OD value of control group) ×100%. Next, the IC50 of the drug was calculated by the inhibition rate.
Cell counting 8 kit cck8
Manufactured by Beyotime
Sourced in China
The Cell Counting 8 Kit (CCK8) is a colorimetric assay for the determination of cell viability and proliferation. The kit utilizes the water-soluble tetrazolium salt WST-8, which is reduced by dehydrogenases in living cells to produce a yellow-colored formazan dye. The amount of formazan dye generated is directly proportional to the number of living cells.
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Lab products found in correlation
2 protocols using cell counting 8 kit cck8
1
Anti-proliferative Effects of S. barbata on Liver Cancer Cells
2
Ferroptosis Induction in Cancer Cells
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HGC-27 and MGC-803 cells were seeded in 96-well plates at 5000 cells/well and cultured at 37 °C and 5% CO2 for 24 h. The ferroptosis inducer FIN56 (Dojindo, Japan) was dissolved in dimethyl sulfoxide to generate working concentrations of 0, 1, 2, 5, 10, 15, and 20 μM; each concentration was evaluated in six wells. Cell viability was measured using a Cell Counting-8 Kit (CCK-8, Beyotime, China). A microplate reader was used to determine the OD at an absorbance of 450 nm. After calculating the half-maximal inhibitory concentration of each cell line, the inhibition curve was plotted using the ggplot2 R tool.
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