Curcumin (C805204), potassium citrate (P816198), EG (E808735), and NH4Cl (A801304) were all purchased from the Macklin Biochemical Technology Co., Ltd. (China, Shanghai). Malondialdehyde (MDA) assay kit (A003-1-2) and superoxide dismutase (SOD) assay kit (A001-3-2) were all from Nanjing Jiancheng Bioengineering Institute (Nanjing, China), osteopontin (OPN) antibody (SAB4501013, Sigma-Aldrich, USA). The fluorescence quantitative polymerase chain reaction (PCR) detection kit (0513471) and reverse transcription kit (0512931) were all from Novoprotein Co., Ltd. (Shanghai, China). Automatic biochemical analyzer (CM-800 Hitachi, Japan), real-time fluorescent quantitative PCR instrument (7500 Fast, Thermo Scientific, USA), optical microscope (DM500, Leica, Germany), microplate reader (Multiskan MK3, Thermo Scientific, USA).
Malondialdehyde mda assay kit a003 1 2
Manufactured by Nanjing Jiancheng
Sourced in China
The Malondialdehyde (MDA) assay kit (A003-1-2) is a quantitative biochemical test used to measure the level of MDA, a biomarker for oxidative stress, in biological samples. The kit utilizes a colorimetric method to determine the MDA concentration.
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Lab products found in correlation
Superoxide dismutase sod assay kit a001 3 2, by Nanjing Jiancheng (2 mentions)
Multiskan mk3, by Thermo Fisher Scientific (1 mentions)
7500 fast, by Thermo Fisher Scientific (1 mentions)
Glutathione peroxidase gsh px assay kit, by Nanjing Jiancheng (1 mentions)
Catalase cat assay kit, by Nanjing Jiancheng (1 mentions)
Cd68 28058 1 ap, by Proteintech (1 mentions)
Af0639, by Affinity Biosciences (1 mentions)
Tissue iron assay kit a039 2 1, by Nanjing Jiancheng (1 mentions)
4 protocols using malondialdehyde mda assay kit a003 1 2
1
Antioxidant and Anti-inflammatory Effects
2
Biochemical and Antioxidant Profiling
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The biochemical indices including serum lipid parameters and anti-oxidative stress levels were determined. The levels of total cholesterol (TC), triglycerides (TG), high-density lipoproteins (HDL-C), and low-density lipoproteins (LDL-C) in blood serum were measured using a total cholesterol assay kit, A111-2-1; triglyceride assay kit, A110-2-1; high-density lipoprotein cholesterol assay kit, A112-2-1; and low-density lipoprotein cholesterol assay kit, A113-2-1 purchased from Nanjing Jiancheng Bioengineering INST, respectively.
The liver tissue was made into a 10% homogenate stock solution and diluted. The anti-oxidative stress indicators of the liver including superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) were measured using Superoxide Dismutase (SOD) assay kit, A001-3-2; Catalase (CAT) assay kit, A007-1-1; Malondialdehyde (MDA) assay kit, A003-1-2; and Glutathione Peroxidase (GSH-PX) assay kit, A005-1-2 purchased from Nanjing Jiancheng Bioengineering INST., respectively.
The liver tissue was made into a 10% homogenate stock solution and diluted. The anti-oxidative stress indicators of the liver including superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and glutathione peroxidase (GSH-Px) were measured using Superoxide Dismutase (SOD) assay kit, A001-3-2; Catalase (CAT) assay kit, A007-1-1; Malondialdehyde (MDA) assay kit, A003-1-2; and Glutathione Peroxidase (GSH-PX) assay kit, A005-1-2 purchased from Nanjing Jiancheng Bioengineering INST., respectively.
3
Liver Redox Status in Heat-Stressed Broilers
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The lipid peroxidation and antioxidants levels in livers were measured to evaluate the liver redox status of heat stressed broilers. The hepatic total superoxide dismutase assay kit (A001-3-2) and malondialdehyde (MDA ) assay kit (A003-1-2) were provided by Nanjing Jiancheng Bioengineering Institute (Nanjing, China). The redox status was measured using the kits according to the manufacturer's instructions.
4
Lipopolysaccharide-Induced Inflammation Protocol
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Lipopolysaccharides (LPS) from Escherichia coli O111:B4 (#L2630) was purchased from Sigma-Aldrich (St Louis, MO, USA). 4-Octyl Itaconate (4-OI) was purchased from MCE (Shanghai, China). Primary antibodies against Nrf2 (AF0639) and GCLM (DF7268) were purchased from Affinity Biosciences and GPX4 (14432-1-AP), SLC7A11 (26864-1-AP), HO-1 (10701-1-AP), PTGS2 (12375-1-AP), and CD68 (28058-1-AP), were purchased from Proteintech Group (Wuhan, China). 4-HNE (bs-6313R) for immunohistochemical staining was purchased from Biosss (Beijing, China). Malondialdehyde (MDA) assay kit (A003-1-2), Total glutathione/Oxidized glutathione assay kit (A061-1-1), and tissue iron assay kit (A039-2-1) were purchased from Jiancheng Bioengineering Institute (Nanjing, China). TNF-α, IL-1β, IL-6 ELISA kits were obtained from Cloud-Clone (Wuhan, China). The ROS Fluorescent Probe Kit was used to detect ROS of tissue (KeyGEN, China) and cell (Biosharp, China). Trypan Blue Staining solution (0.4%) was purchased from Biosharp (Hefei, China)
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