Hydranal water standard 10

Aripiprazole (7-{4-[4-(2,3-dichlorophenyl)-1-piperazinyl]butoxy}-3,4-dihydro-2(1H)-quinolinone) was purchased from HyperChem (Zhejiang, China). Poly(vinyl alcohol) (Poval 4-88), which was used as a film forming polymer and was obtained from Kuraray (Tokyo, Japan). Glycerol (85%) was acquired from Fagron (Cracow, Poland) and glacial acetic acid, Hydra-Point Solvent G and Hydra-Point Titrant 5 mg H₂O/mL, were purchased from Avantor Performance Materials Poland S.A. (Gliwice, Poland). Sodium Acetate Anhydrous, Hydranal^®-Water Standard 10.0 were bought from Sigma-Aldrich (Sigma-Aldrich, Saint Louis, MO, USA). Distilled water was used to prepare all aqueous solutions.

Atlantic salmon (Salmo salar L.) was obtained from the local market (Timişoara, Romania) as a raw product in the spring of 2014. It was an aquaculture product of Norwegian origin. Only the meaty fish parts were used for oil extraction. GC-grade hexane (Sigma-Aldrich) was the main solvent used for raw and degraded fish oil dilutions. The Supelco 37 Component FAME mix (Sigma-Aldrich) and C₈–C₂₀ alkane standard solution (Fluka Chemie AG) were the main tools for identifying the FAMEs in derivatized raw and degraded fish oil. Anhydrous sodium sulfate (p.a., Merck & Co.) was used for drying the raw and degraded fish oil solutions. β-CD hydrate (>98%) from CycloLab (Budapest, Hungary) was used for ASO molecular encapsulation. Ethanol 96% (v/v, Chimopar, Bucharest, Romania) was used for β-CD complexation of ASO. A boron trifluoride–methanol complex (20%, Merck & Co., Inc.) was used for FA derivatization. Finally, Hydranal-Titrant 5, Hydranal-Solvent and Hydranal-Water Standard 10.0 (Sigma-Aldrich, Buchs, Switzerland) were used for the KFT water analysis of β-CD/ASO complexes.