Vironostika hiv 1 uni form 2 ag ab

Testing for HIV was performed by enzyme linked immunosorbent assay (ELISA) in the Mombasa Cohort using the Pishtaz HIV 1.2 ELISA (Pishtaz Teb Diagnostics, Tehran, Iran) for HIV screening and the Vironostika HIV-1 Uni-Form II Ag/Ab (bioMérieux, Marcy I’Etoile, France) for confirmatory testing [10 (link),11 (link)]. In the Mama Salama study, the first-generation Gen-Probe HIV viral load assay (Hologic/Gen-Probe, San Diego, CA, USA) was used for HIV testing [10 (link),12 (link)]. The Gen-Probe APTIMA Combo-2 Assay (Hologic/Gen-Probe, San Diego, CA, USA) was used in both cohorts for diagnosis of infection with Neisseria gonorrhoeae and Chlamydia trachomatis [11 (link),12 (link)]. For both cohorts, BV was diagnosed by Gram stain according to the method of Nugent and Hillier, and T. vaginalis infection was diagnosed by wet preparation [11 (link),12 (link),15 (link)].

Screening for HIV was performed by enzyme linked immunosorbent assay (ELISA). The Detect HIV1–2 ELISA (Biochem Immunosystems) was used from February 1993 through January 2010. Pishtaz HIV 1,2 ELISA (Pishtaz Teb Diagnostics) was used for the remainder of this study. All positive screening ELISA tests were confirmed with a second ELISA (Recombigen HIV 1–2 [Cambridge Biotec] from February 1993 through August 2004, Biorad HIV 1–2 [Biorad Laboratories] from September 2004 through May 2006, and Vironostika HIV-1 Uni-Form II Ag/Ab [bioMerieux] after June 2006).
Screening for STIs included examination of a vaginal saline wet preparation at X40 magnification to identify motile Trichomonas vaginalis parasites. Bacterial vaginosis (BV) was diagnosed by vaginal Gram stain [10 (link)]. Culture of cervical secretions for Neisseria gonorrhoeae was performed on modified Thayer-Martin media. Cervicitis was defined as the presence of an average of ≥30 polymorphonuclear leukocytes per high-power field of Gram stained cervical secretions. Beginning in 2006, additional testing for N. gonorrhoeae and Chlamydia trachomatis was performed using the Gen-Probe APTIMA Combo-2 Assay (Hologic/Gen-Probe). Serological testing for HSV-2 was performed using a type-specific HSV-2 gG based ELISA (HerpeSelect 2, Focus Diagnostics). Index values ≥2.1 were considered positive [11 (link)].