Goat anti mouse igg horseradish peroxidase conjugate goat antimouse igg hrp a4416

Poly(diallyldimethylammonium chloride) (PDDA, No. 17338; Polyscience Inc., Niles, IL, USA; M_w = 2.4 × 10⁵ g/mol) is a strong polyelectrolyte with a positive charge, and poly(acrylic acid) (PAA, No. 181285; Sigma, St. Louis, MO, USA; M_w = 4.5 × 10⁵ g/mol) is a weak polyelectrolyte with a negative charge. Rabbit antimouse IgG (M7023), mouse IgG (I5381), goat anti-mouse IgG-horseradish peroxidase conjugate (goat antimouse IgG-HRP; A4416), ovalbumin from chicken egg white (OVA, A5503), and N-hydroxysuccinimide (NHS) were acquired from Sigma (St. Louis, MO, USA). 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Dojindo Co. Ltd., Shanghai, China. Disodium hydrogen phosphate and monometallic sodium orthophosphate were purchased from Beijing Chemical Reagent Co. Ltd. (Beijing, China). Tris(hydroxymethyl) aminomethane hydrochloride (Tris-HCl; Acros Organics, Fair Lawn, NJ, USA) was used in the preparation of a buffer solution. 96 wells PS microplate (Costar 3599) was purchased from Corning Incorporated (Corning, NY, USA). The chemicals were used without further purification. Ultrapure water was used throughout the experiments.

PDDA (#17338; Polyscience Inc., Niles, IL, USA; Mw = 2.4 × 10⁵ g/mol) and PSS (#416888; Alfa Aesar, Ward Hill, MA, USA; Mw = 7.0 × 10⁴ g/mol) were used as polyelectrolytes. The diameter and pore size of the CA membrane filter (C020A045A; Advantec Toyo Roshi Kaisha, Ltd., Tokyo, Japan) were 13 mm and 450 nm, respectively. Rabbit anti-mouse IgG (M7023), mouse IgG (I5381), goat anti-mouse IgG-horseradish peroxidase conjugate (goat antimouse IgG-HRP; A4416), ovalbumin from chicken egg white (OVA, A5503) and N-Hydroxysuccinimid (NHS) were acquired from Sigma, St Louis, MO, USA. 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) was purchased from Dojindo Co. Ltd., Shanghai, China. Disodium hydrogen phosphate and monometallic sodium orthophosphate were purchased from Beijing Chemical Reagent Co. Ltd., Beijing, China. Carboxylated PS NPs (PS-01-200, diameter 200 nm) were purchased from Janus New-Materials Co. Ltd., Nanjing, China. Tris(hydroxymethyl) aminomethane hydrochloride (Tris-HCl; Acros Organics, Fair Lawn, NJ, USA) was used in the preparation of a buffer solution. The chemicals were used in the experiments without further purification. Ultrapure water was used throughout the experiments.