Random hexamer priming kit

Manufactured by Thermo Fisher Scientific

Sourced in France

The Random Hexamer Priming Kit is a laboratory reagent used for the reverse transcription of RNA into complementary DNA (cDNA). The kit contains a mixture of random hexamer oligonucleotides, which can initiate cDNA synthesis from any region of the RNA template, allowing for the generation of full-length cDNA from diverse RNA samples.

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Lab products found in correlation

Cfx manager software, by Bio-Rad (2 mentions) Taqman probe, by Thermo Fisher Scientific (2 mentions) Cfx96 real time pcr detection system, by Bio-Rad (2 mentions)

Spelling variants of this product

Random hexamers (967 citations) Random hexamer priming (11 citations)

2 protocols using random hexamer priming kit

Quantifying Gene Expression in Cultured Arteries

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Total RNA was extracted from cultured artery and cDNA obtained by reverse transcription using a random hexamer priming kit (Thermo Fisher Scientific, Applied Bioscience) in a final volume of 100 µL. Then, specific pre-developed TaqMan probes from Thermo Fisher Scientific (TaqMan Gene Expression Assays) were used for PCR amplification (listed in Supplementary Table 2). Fluorescence was detected with the CFX96TM Real-Time PCR Detection System and the results analyzed with the CFX ManagerTM software (Bio-Rad Laboratories). Gene expression was normalized to the expression of the endogenous control GUSB using the comparative ΔCt method. mRNA concentration was expressed in relative units with respect to GUSB expression (relative expression).

Samson M., Genet C., Corbera-Bellalta M., Greigert H., Espígol-Frigolé G., Gérard C., Cladière C., Alba-Rovira R., Ciudad M., Gabrielle P.H., Creuzot-Garcher C., Tarris G., Martin L., Saas P., Audia S., Bonnotte B, & Cid M.C. (2023). Human monocyte-derived suppressive cells (HuMoSC) for cell therapy in giant cell arteritis. Frontiers in Immunology, 14, 1137794.

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Quantitative Real-Time PCR Analysis of Gene Expression

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Total RNA was extracted from cultured artery and cDNA obtained by reverse transcription employing a random hexamer priming kit (Thermo Fisher Scientific, Applied Bioscience, VILLEBON‐SUR‐YVETTE, France) in a final volume of 100 µL. Then, specific pre‐developed TaqMan probes from Thermo Fisher Scientific (TaqMan Gene Expression Assays) were used for PCR amplification of GUSB, FoxP3 and IL6 (Supplementary table 1). Fluorescence was detected with CFX96TM Real‐Time PCR Detection System, and results were analysed with CFX ManagerTM software (Bio‐Rad Laboratories). Gene expression was normalised to the expression of the endogenous control GUSB using the comparative ΔCt method. mRNA concentration was expressed in relative units with respect to GUSB expression (relative expression).

Samson M., Greigert H., Ciudad M., Gerard C., Ghesquière T., Trad M., Corbera‐Bellalta M., Genet C., Ouandji S., Cladière C., Thebault M., Ly K.H., Liozon E., Maurier F., Bienvenu B., Terrier B., Guillevin L., Charles P., Quipourt V., Devilliers H., Gabrielle P., Creuzot‐Garcher C., Tarris G., Martin L., Saas P., Audia S., Cid M.C, & Bonnotte B. (2021). Improvement of Treg immune response after treatment with tocilizumab in giant cell arteritis. Clinical & Translational Immunology, 10(9), e1332.

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