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Sc 28383

Manufactured by Cell Signaling Technology
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Sc-28383 is a lab equipment product offered by Cell Signaling Technology. It is a specific antibody used for research purposes, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach without interpretation or extrapolation.

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Lab products found in correlation

Nitrocellulose membrane, by Bio-Rad (2 mentions) Foxk2, by Fortis Life Sciences (2 mentions) β actin, by Abcam (2 mentions) Ab8226, by Merck Group (2 mentions) Slc7a11, by Cell Signaling Technology (2 mentions) H2aub, by Merck Group (2 mentions) V4505, by Abcam (2 mentions) Foxk1, by Abcam (2 mentions) Hcfc1, by Fortis Life Sciences (2 mentions) Kdm1b, by Abcam (2 mentions) Asxl1, by Santa Cruz Biotechnology (2 mentions) Sc 293204, by Santa Cruz Biotechnology (2 mentions)

2 protocols using sc 28383

1

Immunoprecipitation and Western Blot Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoprecipitation was conducted as previously described 62 (link), 63 (link). Protein extracts were resolved by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad) using standard techniques. The primary antibodies and concentrations used for Western blotting were: BAP1 (1:1000; Santa Cruz, sc-28383), SLC7A11 (1:5000; Cell Signaling Technology, 12691), tubulin (1:5000; Cell Signaling Technology, 2144), H2Aub (1:5000; Millipore, 05–678), H2A (1:5000; Millipore, ABE327), vinculin (1:10000; Sigma, V4505), FOXK1 (1:1000; Abcam, ab18196 ), FOXK2 (1:1000; Bethyl Laboratories, A301–730A), HCFC1 (1:1000; Bethyl Laboratories, A301–399A), OGT (1:1000, Cell Signaling Technology, 5368), KDM1B (1:1000; Abcam, ab193080), ASXL1 (1:1000; Santa Cruz, sc-293204), p53 (1:1000; Santa Cruz, sc-126), PARP (1:1000; Cell Signaling Technology, 9542), β-actin (1:4000; Abcam, ab8226), and V5 (1:2000; Sigma, A8012).
Zhang Y., Shi J., Liu X., Feng L., Gong Z., Koppula P., Sirohi K., Li X., Wei Y., Lee H., Zhuang L., Chen G., Xiao Z.D., Hung M.C., Chen J., Huang P., Li W, & Gan B. (2018). BAP1 links metabolic regulation of ferroptosis to tumor suppression. Nature cell biology, 20(10), 1181-1192.
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2

Immunoprecipitation and Western Blot Analysis

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Immunoprecipitation was conducted as previously described 62 (link), 63 (link). Protein extracts were resolved by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad) using standard techniques. The primary antibodies and concentrations used for Western blotting were: BAP1 (1:1000; Santa Cruz, sc-28383), SLC7A11 (1:5000; Cell Signaling Technology, 12691), tubulin (1:5000; Cell Signaling Technology, 2144), H2Aub (1:5000; Millipore, 05–678), H2A (1:5000; Millipore, ABE327), vinculin (1:10000; Sigma, V4505), FOXK1 (1:1000; Abcam, ab18196 ), FOXK2 (1:1000; Bethyl Laboratories, A301–730A), HCFC1 (1:1000; Bethyl Laboratories, A301–399A), OGT (1:1000, Cell Signaling Technology, 5368), KDM1B (1:1000; Abcam, ab193080), ASXL1 (1:1000; Santa Cruz, sc-293204), p53 (1:1000; Santa Cruz, sc-126), PARP (1:1000; Cell Signaling Technology, 9542), β-actin (1:4000; Abcam, ab8226), and V5 (1:2000; Sigma, A8012).
Zhang Y., Shi J., Liu X., Feng L., Gong Z., Koppula P., Sirohi K., Li X., Wei Y., Lee H., Zhuang L., Chen G., Xiao Z.D., Hung M.C., Chen J., Huang P., Li W, & Gan B. (2018). BAP1 links metabolic regulation of ferroptosis to tumor suppression. Nature cell biology, 20(10), 1181-1192.
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