Apollo reaction reagent

Manufactured by RiboBio

The Apollo reaction reagent is a laboratory product designed for use in various scientific applications. It serves as a core component in specific experimental protocols, enabling researchers to carry out essential reactions and analyses. The reagent's primary function is to facilitate targeted chemical reactions, supporting the advancement of scientific research across diverse fields.

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Lab products found in correlation

5 ethynyl 2 deoxyuridine (edu), by RiboBio (3 mentions) Triton x 100, by Merck Group (2 mentions) Dmi4000 microscope, by Leica (2 mentions) Hoechst 33342, by Merck Group (2 mentions) Glycine, by Avantor (2 mentions) Chamber slide, by Merck Group (2 mentions) Tcs sp5, by Leica (1 mentions) Triton x 100, by Solarbio (1 mentions) Hoechst 33342, by RiboBio (1 mentions)

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Apollo reagent (2 citations)

3 protocols using apollo reaction reagent

Cell Proliferation Quantification via EdU Assay

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Cell proliferation was measured using EdU assay. In brief, the cells were cultured in chamber slides (Millipore, Billerica, MA, USA; 2 × 10⁴ cells/well). Forty-eight hours after culture, the cells were treated with 50 μM EdU (RiboBio) for an additional 2 h at 37 °C, and then were fixed with 4% formaldehyde for 30 min, followed by addition of 200 μl glycine (2 mg/ml; Amresco, OH, USA). After 5 min, the cells were incubated with 0.5% Triton X-100 (Sigma-Aldrich, St. Louis, MO, USA) for 10 min at room temperature. Following washing with PBS for 5 min, 1 × Apollo reaction reagent (RiboBio) was added and incubated at room temperature in the dark for 30 min, and then the cells were stained with 200 μl Hoechst 33342 (5 μg/ml; Sigma-Aldrich) for an additional 30 min in the dark. Cells labeled and unlabeled by EdU were counted under a Leica DMI4000 microscope, and pictures were taken. The assay was performed three times in triplicate.

Zheng Z., Qu J.Q., Yi H.M., Ye X., Huang W., Xiao T., Li J.Y., Wang Y.Y., Feng J., Zhu J.F., Lu S.S., Yi H, & Xiao Z.Q. (2017). MiR-125b regulates proliferation and apoptosis of nasopharyngeal carcinoma by targeting A20/NF-κB signaling pathway. Cell Death & Disease, 8(6), e2855-.

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EdU Proliferation Assay Protocol

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Cell proliferation was measured using EdU assay. Briefly, the cells were cultured in chamber slides (Millipore; 2 × 10⁴ cells/well). 48 h after culture, the cells were treated with 50 µM EdU (RiboBio, Guanzhou, China) for an additional 2 h at 37 °C, and then were fixed with 4% formaldehyde for 30 min, followed by addition of 200 µl glycine (2 mg/ml; Amresco). After 5 min, the cells were incubated with 0.5% Triton X-100 (Sigma-Aldrich) for 10 min at room temperature. Following washing with PBS for 5 min, 1 × Apollo reaction reagent (RiboBio) was added and incubated at RT in the dark for 30 min, and then the cells were stained with 200 µl Hoechst 33342 (5 µg/ml; Sigma-Aldrich) for an additional 30 min in the dark. Cells labeled and unlabeled by EdU were counted under a Leica DMI4000 microscope, and pictures were taken.

Xiao T., Zhu W., Huang W., Lu S.S., Li X.H., Xiao Z.Q, & Yi H. (2018). RACK1 promotes tumorigenicity of colon cancer by inducing cell autophagy. Cell Death & Disease, 9(12), 1148.

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Cell Proliferation Assay with EdU and Confocal Microscopy

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The cells were cultured on coverslips in 24-well plates (2×10⁴ cells/well) and stimulated with TSA. After 24, 48, 72 and 96 h, the cells were treated with 50 µM EdU (Guangzhou RiboBio, Co., Ltd., Guangzhou, China) for an additional 2 h at 37°C. Following treatment, the culture medium was discarded and the cells were washed twice with Hyclone phosphate-buffered saline (PBS; GE Healthcare Life Sciences, Logan, UT, USA). The cells were fixed with 4% formaldehyde (Beijing Zhongyuan Huadun Technology Trade Co., Ltd., Beijing, China) for 30 min, followed by addition of 200 µl glycine (2 mg/ml; Amresco, LLC, Solon, OH, USA) to each well. After 5 min, the cells were washed twice with PBS and incubated with 0.5% Triton X-100 (Beijing Solarbio Science & Technology Co., Ltd., Beijing, China) for 10 min at room temperature. Following washing with PBS for 5 min, 1X Apollo reaction reagent (Guangzhou RiboBio Co., Ltd.) was added (200 µl/well) and the plates were incubated at room temperature in the dark for 30 min, following which the cells were stained with 200 µl Hoechst 33342 (5 µg/ml; Guangzhou RiboBio Co., Ltd.) for an additional 30 min in the dark. Subsequent to washing with PBS twice, the cells were analyzed using a laser-scanning confocal microscope (TCS SP5; Leica Microsystems GmbH, Wetzlar, Germany).

ZE X., JIA J., LI X., YOU H., ZHAO X., ZHANG D, & WANG B. (2015). Tanshinone IIA promotes the proliferation of WB-F344 hepatic oval cells via Wnt/β-catenin signaling. Molecular Medicine Reports, 13(2), 1501-1508.

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