All participants completed a uniform questionnaire detailing history of diabetes, medication use, smoking status, and alcohol consumption. Body height and weight were measured without shoes and outer clothing. BMI was calculated according to weight in kilograms divided by square of height in meters. Waist circumference was measured using a soft tape at midway between the lowest rib and iliac crest in standing position. All blood samples were obtained after at least 12 h of fasting. Serum total cholesterol, HDL-cholesterol, triglyceride(TG) were measured using oxidase method32 (link). LDL cholesterol was calculated using the Friedewald equation. Liver enzymes (ALT, AST) were measured by Ultraviolet (UV) lactate and malate dehydrogenase methods on a model 7600 automated bio-analyser (Hitachi, Tokyo, Japan). Plasma glucose concentrations were measured using glucose oxidase method. Serum insulin concentrations were determined using an electrochemiluminescence immunoassay33 (link).
Model 7600 automated bio analyser
Manufactured by Hitachi
Sourced in Japan
The Hitachi Model 7600 is an automated bio-analyzer designed for clinical laboratory applications. The device performs a variety of biochemical analyses on biological samples, such as serum, plasma, and urine. The Model 7600 automates the analytical process, from sample preparation to data analysis, ensuring consistent and reliable results.
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Lab products found in correlation
2 protocols using model 7600 automated bio analyser
1
Comprehensive Metabolic Profiling Protocol
2
Comprehensive Metabolic Profiling in Clinical Research
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Blood samples were obtained from each patient after a fasting period of at least 10 hours. Total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), triglyceride (TG), creatinine (Cr), and liver enzyme levels were measured using a Model 7600 Automated Bio-Analyser (Hitachi, Tokyo, Japan). Low density lipoprotein cholesterol (LDL-C) level was calculated using the Friedewald equation [26 (link)]. Fasting blood glucose (FBG) and 2-hour post-load plasma glucose (PPG) levels following a 75-g oral glucose challenge for participants without diabetes were measured with the glucose oxidase method.
Serum 25(OH)D and PTH levels were measured using a Roche Cobas E601 electro chemiluminescence system with complete reagents (Roche Diagnostics, Basel, Switzerland). Intra- and inter-batch variation coefficients for 25(OH)D and PTH were below 10% and 5%, respectively. The eGFR was estimated based on the serum Cr concentration using the Modification of MDRD formulation, i.e., eGFR (mL/min/1.73 m2)=186× Scr−1.154×age−0.203×0.742 (if female)×1.233 (if Chinese) [27 (link)].
Serum 25(OH)D and PTH levels were measured using a Roche Cobas E601 electro chemiluminescence system with complete reagents (Roche Diagnostics, Basel, Switzerland). Intra- and inter-batch variation coefficients for 25(OH)D and PTH were below 10% and 5%, respectively. The eGFR was estimated based on the serum Cr concentration using the Modification of MDRD formulation, i.e., eGFR (mL/min/1.73 m2)=186× Scr−1.154×age−0.203×0.742 (if female)×1.233 (if Chinese) [27 (link)].
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