Food-grade sodium alginate was obtained from Farmalabor, non-ionic surface-active agent polyoxyethylene (20) sorbitan monooleate (Tween 80), calcium chloride and citric acid were purchased from Sigma-Aldrich while Lemongrass (Cymbopogon nardus) Essential Oil (LEO) (Erbamea, San Giustino, Perugia, Italia) was purchased from a local shop. Fuji apples were purchased in a local supermarket. For all the preparations, ultra-pure water was used.
Polyoxyethylene 20 sorbitan monooleate tween 80
Manufactured by Merck Group
Sourced in United States, Germany
Polyoxyethylene (20) sorbitan monooleate, also known as Tween 80, is a non-ionic surfactant commonly used as a laboratory reagent. It acts as a detergent, emulsifier, and wetting agent. Tween 80 is soluble in water and is often used to improve the solubility and stability of various substances in aqueous solutions.
Automatically generated - may contain errors
Lab products found in correlation
Milli q water, by Merck Group (2 mentions)
Polyoxyethylene 20 sorbitan monolaurate tween 20, by Merck Group (2 mentions)
Acetic acid, by Merck Group (2 mentions)
Chrysin, by Merck Group (1 mentions)
Milli q water purification system, by Merck Group (1 mentions)
Absolute ethanol, by Merck Group (1 mentions)
Pluronic f127, by BASF (1 mentions)
Kh2po4, by Kemika (1 mentions)
Croton oil, by Merck Group (1 mentions)
Polyvinyl alcohol (pva), by Thermo Fisher Scientific (1 mentions)
Sorbitan monooleate span 80, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox, by Merck Group (1 mentions)
Phosphoric acid hplc grade, by Merck Group (1 mentions)
Hplc grade acetonitrile, by Merck Group (1 mentions)
Streptomycin sulfate, by Merck Group (1 mentions)
Cholesterol chol, by Merck Group (1 mentions)
16 protocols using polyoxyethylene 20 sorbitan monooleate tween 80
1
Alginate-based Edible Coating for Apples
2
Cyst Cultivation and Encapsulation
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Chrysin and polyoxyethylene 20 sorbitan monooleate (Tween 80) were purchased from Sigma-Aldrich, St. Louis, MO, USA. Absolute ethanol was purchased from Merck Millipore (Darmstadt, Germany). Moina siamensis cysts were purchased from the ornamental fish market in Lampang, Thailand. Pluronic F-127, a trade name of poloxamer 407, was from BASF Ludwigshafen, Germany. Nylon filter membrane was purchased from Anpel Laboratory Technologies (Shanghai) Inc. (Shanghai, Chaina). Milli-Q water was obtained from a Milli-Q water purification system manufactured by Millipore (Billerica, MA, USA). All solvents and other materials were of analytical grade.
3
Formulation and Characterization of AZT Liposomes
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Lipoid E PC S (from egg; phosphatidylcholine content ≥ 96%) (EPC) and egg phosphatidylglycerol (EPG) were generous gifts from Lipoid GmbH (Ludwigshafen, Germany), while AZT in the form of a dihydrate was kindly donated by PLIVA Croatia Ltd. (Zagreb, Croatia). Dimethyldioctadecylammonium bromide (DODAB) and polyoxyethylene(20) sorbitanmonooleate (Tween 80) were provided by Sigma–Aldrich (St. Louis, MO, USA). Ethanol and KH2PO4 were purchased from Kemika (Zagreb, Croatia). For the preparation of liposomes, 0.01 M phosphate buffer (PB) was prepared by dissolving 1.3609 g KH2PO4 in demineralized water up to 1000 mL, and the pH was adjusted to 7.5 by the addition of 10 M KOH [19 (link)].
4
Formulation and Evaluation of Anti-Inflammatory Gel
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CEO was obtained from Wako JAPAN, 036–03562, Lot KP J4110. Glycerol monoacetate (GMA) was acquired from Koch-Light Laboratories Ltd., Colnbrook Bucks, England. Polyoxyethylene (20) sorbitan monooleate (tween 80®) and croton oil were purchased from Sigma-Aldrich, Saint Louis, MO, USA while caprylocaproyl macrogol-8-glyceride (Labrasol) was obtained as a gift sample from Gattefossé, St Priest, France. PVA with a molecular weight (MW) of 146–186 kilodaltons (KDa) and a degree of hydrolysis of 98.0–98.8 moL % was purchased from Acros Organics, New Jersey, USA. Dimethyl sulfoxide (DMSO) with an MW of 78.129 g/moL was obtained from SDFCL, Mumbai-400030, India. GG was supplied by Premcem Gums Ltd., India while low MW CS (1526.454 g/moL) with a deacetylation degree of 90–95% was obtained from Oxford Chemical Co., Mumbai, India. Absolute ethyl alcohol, analytical grade of glacial acetic acid (99%), acetone, potassium phosphate monobasic (KH2PO4), disodium hydrogen phosphate (Na2HPO4), sodium chloride (NaCl) and GA were procured from El-Nasr Pharmaceutical Chemical Co., Cairo, Egypt. Primary anti-COX-2 (Polyclonal PA137504) was obtained from Thermo Fisher Scientific, Waltham, MA, USA while Universal kit [secondary antibody and 0.04% 3, 3ʹ-diaminobenzidine tetrahydrochloride (DAB)] was procured from DAKO, Denmark.
5
Olive Mill Wastewater Characterization and Antioxidant Potential
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Olive mill wastewater (OMW) used in this study was obtained by means of a three-phase olive oil extraction and centrifugation system and was kindly provided by Mamalan Agro Industrial Company (Zanjan, Iran). Miglyol 812 oil, a mixture of C6-C12 medium chain triglycerides (MCT), was supplied by Sasol GmbH (Hamburg, Germany). Folin-Ciocalteu reagent and hydrochloric acid (37%) were purchased from VWR International Eurolab (Llinars del Vallès, Spain) and Acros Organics (Geel, Belgium), respectively. Sodium carbonate, methanol, gallic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), sorbitan monooleate (Span 80) and polyoxyethylene (20) sorbitan monooleate (Tween 80) were purchased from Sigma-Aldrich (Darmstadt, Germany). Milli-Q water (Millipore, St. Louis, MO, USA) was used in all samples.
6
Formulation and Evaluation of Minoxidil-Loaded Nanoemulsion
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SV was kindly provided by Western pharmaceutical company (Cairo, Egypt). Sorbitan monostearate (Span 60) was obtained from PureLab, USA. Polyoxyethylene (20) sorbitan monooleate (Tween80) and PEG-40 hydrogenated castor oil (Cremophor RH 40) were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Monobasic potassium phosphate and dibasic potassium phosphate were purchased from Alpha Chemica (Mumbai, India). Absolute ethanol was obtained from El-Nasr Pharmaceutical Chemical Company (Cairo, Egypt). Spectra/Pore® dialysis membrane (12,000–14,000 Mwt cut-off) was purchased from Spectrum Laboratories Inc. (Irving, TX, USA). Acetonitrile (HPLC grade) and phosphoric acid (HPLC grade) were purchased from Sigma-Aldrich Chemical Co. (St. Louis, MO, USA). All other chemicals were of analytical grade and used as received. Marketed 5% minoxidil lotion (Hair Plus Back®) was purchased from Minapharm Pharmaceuticals (Cairo, Egypt).
7
Curcumin-Based Antiviral Liposome Formulation
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Curcumin, Polyoxyethylene (20) sorbitan monooleate (Tween 80), and cholesterol (CHOL) were obtained from Sigma Chemical Co. (St. Louis, MO, USA). Sorbitan monostearate (Span 60) was purchased from Oxford Lab Chemicals (Mumbai, India). Potassium monohydrogen phosphate and potassium dihydrogen phosphate were obtained from Alpha Chemica (Mumbai, India). Chloroform was obtained from El-Nasr Pharmaceutical Chemical Company (Cairo, Egypt). Spectra/Pore® dialysis membranes (Spectra/pore 4, 12,000–14,000 Mwt cut-off, diameter 16 mm) were purchased from Spectrum Laboratories Inc. (Rancho Dominguez, CA, USA). HS-1 virus was kindly provided by Dr. R.G. Hughes (Roswell Park Memorial Institute, Buffalo, New York, NY, USA). Vero cells (African green monkey kidney cells, Vero C1008 [Vero 76, clone E6, Vero E6]) were purchased from Viromed Laboratories (Minnetonka, MN, USA). Dulbecco’s modified Eagle’s medium (DMEM), Penicillin G, and streptomycin sulfate were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Calf serum was obtained from HyClone Laboratories, Inc. (Logan, UT, USA). All other chemicals and solvents were of analytical grade and used as received.
8
Antioxidant and Collagenase Inhibition Assay
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L-Ascorbic acid, ferrous sulfate (FeSO4), oleanolic acid, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,4,6-tris(2-pyridyl)-striazine (TPTZ), collagenase from Clostridium histolyticum (EC 3.4.24.3), synthetic peptide 2-furanacryloyl-Leu-Gly-Pro-Ala (FALGPA), N-tris(hydroxymethyl)methylglycine (tricine), acetic acid, hydrochloric acid (HCl), polyoxyethylene (20) sorbitan monolaurate (Tween® 20), polyoxyethylene (20) sorbitan monooleate (Tween® 80), polyoxyethylene sorbitan trioleate (Tween® 85), soy lecithin, ethanol, and dimethyl sulfoxide (DMSO) were analytical grade and purchased from Sigma-Aldrich (St. Louise, MO, USA). Mineral oil, olive oil, glycerin, propylene glycol, butylene glycol, and sorbitol were cosmetic grade and purchased from Namsiang Co., Ltd. (Bangkok, Thailand).
9
Fenofibrate Bioavailability Enhancement Formulation
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Fenofibrate, aminopropyltriethoxysilane (APTES, 99%), sucrose, polyoxyethylene (20)-sorbitan monooleate (Tween® 80), pancreatin (8× US Pharmacopeia [USP] standard), and sodium taurodeoxycholate (NaTDC) were purchased from Sigma-Aldrich (St Louis, MO, USA). Lecithin (~99.2% soybean-phosphatidylcholine) was purchased from Avanti Polar Lipids (Alabaster, AL, USA). Superba™ Krill Oil was kindly supplied by Aker BioMarine AS (Oslo, Norway). Tertiary butyl alcohol (TBA), magnesium chloride hexahydrate, sodium chloride, hydrochloric acid, potassium dihydrogen phosphate, sodium hydrogen phosphate, and phosphoric acid were obtained from Junsei Chemical Co. Ltd. (Tokyo, Japan). Acetonitrile was high-performance liquid chromatography (HPLC) grade (Merck, Darmstadt, Germany).
10
Transfersome and Liposome Formulation Protocol
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Analytical grade Chloroform of Merck and analytical grade Methanol of Sigma Andrich were used as organic solvents for the formulation of transfersome and liposomes. Absolute Hydrochloric acid (Merck) and Sodium Hydroxide (Sigma-Aldrich, Germany) were used for the quantitative analysis of Glucosamine sulfate as per official compendia method. Triethylamine procured from Merck was used for the formulation of hydrogel. Eugenol was purchased from Sigma Scientific Pvt limited. Phosphate Buffer saline (PBS) solution having PH. 7.0 was made from PBS tablets purchased from Sigma Scientific. Distilled water was taken from pharmaceutics lab, Department of Pharmacy, Riphah International University (Islamabad, Pakistan).
For the formulation of transfersome, Phospholipid 90G was generously gifted by Lipoid Germany, Glucosamine sulfate was gifted from Polyline ChemPharma Peshawar, Polyoxyethylene (20) sorbitan monooleate (Tween 80) and sorbitan monostearate (Span 60) were procured from Merck. For the induction of osteoarthritis in knee joint of rabbitsl -Cysteine and Papain were procured from Sigma Adrich Germany. All the chemicals were of pure analytical grade.
For the formulation of transfersome, Phospholipid 90G was generously gifted by Lipoid Germany, Glucosamine sulfate was gifted from Polyline ChemPharma Peshawar, Polyoxyethylene (20) sorbitan monooleate (Tween 80) and sorbitan monostearate (Span 60) were procured from Merck. For the induction of osteoarthritis in knee joint of rabbits
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