Mouse monoclonal gfp

Manufactured by Santa Cruz Biotechnology

Mouse monoclonal GFP is a laboratory reagent that produces a green fluorescent protein from the jellyfish Aequorea victoria. It can be used to tag and visualize proteins in cell and tissue samples.

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Lab products found in correlation

Mouse monoclonal phospholamban, by Merck Group (1 mentions) Anti c myc, by Abcam (1 mentions) Mouse monoclonal anti flag m2, by Merck Group (1 mentions) Rabbit polyclonal anti flag, by Merck Group (1 mentions) Rat monoclonal peroxidase conjugated anti ha 3f10, by Roche (1 mentions) Rabbit polyclonal cleaved caspase 3 antibody, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Mouse monoclonal anti-GFP (17 citations) Mouse monoclonal anti-GFP antibody (11 citations) Mouse monoclonal anti-GFP (B-2, (7 citations) Mouse monoclonal GFP antibody (2 citations) Mouse monoclonal to GFP/YFP (2 citations)

3 protocols using mouse monoclonal gfp

Protein Immunoblotting and Immunoprecipitation

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The following primary antibodies were used for immunoblotting: mouse monoclonal Phospholamban (Millipore; 1:1000 dilution), polyclonal phosphor-phospholaman serine 16 (Millipore; 1:500), mouse monoclonal GFP (Santa Cruz Biotechnology; 1:500 dilution), polyclonal mCherry (Thermo Scientific Pierce; 1:3000 dilution), monoclonal PKA RIIα subunit (Santa Cruz Biotechnology; 1:500).
Immunoprecipitations were carried out using the following antibodies: polyclonal AKAP7 (Sigma; 5 µg), mouse monoclonal GFP (Santa Cruz Biotechnology; 5 µg), mouse monoclonal Phospholamban (Millipore; 3 µg)

Rigatti M., Le A.V., Gerber C., Moraru I.I, & Dodge-Kafka K.L. (2015). Phosphorylation state-dependent interaction between AKAP7δ/γ and phospholamban increases phospholamban phosphorylation. Cellular signalling, 27(9), 1807-1815.

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Antibodies for Protein Detection

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Rabbit polyclonal anti-Sam35 (Chan and Lithgow, 2008 (link)) was a generous gift from Trevor Lithgow. Rabbit polyclonal anti-Sis1 (Yan and Craig, 1999 (link)) was a generous gift from Elizabeth Craig. Rabbit polyclonal anti-Cue1 and anti-ubiquitin were described previously (Kostova et al., 2009 (link)). Commercial antibodies used were: mouse monoclonal PORIN (MTCO1; abcam); rabbit polyclonal Prc1 (CPY; abcam); mouse monoclonal GFP (Santa Cruz Biotechnology); mouse monoclonal phosphoglycerate kinase 1 (PGK; Life Technologies); rat monoclonal peroxidase-conjugated anti-HA (3F10; Roche); mouse monoclonal anti-FLAG (M2; Sigma-Aldrich); rabbit polyclonal anti-FLAG (Sigma-Aldrich); and rabbit polyclonal anti-c-myc (Abcam).

Metzger M.B., Scales J.L., Dunklebarger M.F., Loncarek J, & Weissman A.M. (2020). A protein quality control pathway at the mitochondrial outer membrane. eLife, 9, e51065.

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Immunodetection with Various Antibodies

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Mouse monoclonal and rabbit polyclonal HA, mouse monoclonal GFP and rabbit polyclonal cdk2 antibodies were from Santa Cruz Biotechnology. Rabbit polyclonal cleaved caspase-3 antibody was from Cell Signaling Technology. Mouse monoclonal TFR antibody and rabbit polyclonal TBC1D17 antibody were obtained from Invitrogen. Rabbit polyclonal p62/SQSTM1 antibody was from Sigma-Aldrich and mouse monoclonal LC3 antibody was from Enzo Life Sciences. Secondary antibodies Cy-3 conjugated anti-mouse and anti-rabbit IgG, and HRP conjugated anti-mouse and anti-rabbit IgGs were from Amersham. Alexa-488 and Alexa-633 conjugated anti-mouse and anti-rabbit IgGs were from Molecular Probes.

Chalasani M.L., Kumari A., Radha V, & Swarup G. (2014). E50K-OPTN-Induced Retinal Cell Death Involves the Rab GTPase-Activating Protein, TBC1D17 Mediated Block in Autophagy. PLoS ONE, 9(4), e95758.

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