Anti β glactosidase

Manufactured by Abcam

Anti-β-galactosidase is a lab equipment product that can be used to detect and quantify the presence of β-galactosidase, an enzyme found in various organisms. The product functions by inhibiting the activity of β-galactosidase, which can be useful in various research and diagnostic applications.

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Lab products found in correlation

Dynalight 488, by Abcam (2 mentions) Alexa fluor 546, by Thermo Fisher Scientific (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Anti neuronal class 3 β tubulin, by Fortrea (2 mentions) Anti e cadherin, by BD (2 mentions) Reddot2, by Biotium (1 mentions) Ab9361, by BD (1 mentions) Anti cytokeratin 5, by Abcam (1 mentions) Alexa fluor 660, by Thermo Fisher Scientific (1 mentions)

2 protocols using anti β glactosidase

Whole Mount Immunostaining of Salivary Glands

Cited 1 time

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Whole mount SMG tissues were immunostained as previously described (Sandell et al., 2014 (link)). Primary antibodies used were anti-β-glactosidase (Abcam ab9361) 1:500, anti-E-cadherin (BD Biosciences #610182) 1:50, anti-Neuronal Class III β-Tubulin (Covance, PRB0435-P) 1:1000. Fluorescently conjugated secondary antibodies Alexafluor 488, AlexaFluor 546 (Invitrogen), or Dynalight 488 (Abcam) were used at 1:300. Nuclei were stained with RedDot 2 (Biotium).

Wright D.M., Buenger D.E., Abashev T.M., Lindeman R.P., Ding J, & Sandell L.L. (2015). Retinoic Acid Regulates Embryonic Development of Mammalian Submandibular Salivary Glands. Developmental biology, 407(1), 57-67.

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Multicolor Immunofluorescent Staining

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Primary antibodies used were:
anti-β-glactosidase (Abcam ab9361) 1:500,
anti-E-cadherin (BD Biosciences #610182) 1:50,
anti-Neuronal Class III β-Tubulin (Covance, PRB0435-P) 1:1000,
anti-Cytokeratin 5, (Abcam ab24647)1:1000.
anti-Cytokeratin-8 (DSHB TROMA-I)1:50.
Fluorescently conjugated secondary antibodies, each used at 1:300 were: Alexafluor 488, AlexaFluor 546, AlexaFluor 660 (Invitrogen), or Dynalight 488 (Abcam).

Abashev T.M., Metzler M.A., Wright D.M, & Sandell L.L. (2017). Retinoic Acid signaling regulates Krt5 and Krt14 independently of stem cell markers in submandibular salivary gland epithelium. Developmental dynamics : an official publication of the American Association of Anatomists, 246(2), 135-147.

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