Anti vcam 1 antibody

Manufactured by Southern Biotech

Anti–VCAM-1 antibody is a laboratory reagent used for the detection and quantification of vascular cell adhesion molecule 1 (VCAM-1) in biological samples. VCAM-1 is an important cell surface protein involved in various cellular processes, and its measurement can provide insights into various physiological and pathological conditions.

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Lab products found in correlation

Streptozotocin (stz), by Merck Group (1 mentions) Superfrost plus, by Thermo Fisher Scientific (1 mentions) Alexa fluor 555, by Cell Signaling Technology (1 mentions)

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VCAM-1 (2 citations)

2 protocols using anti vcam 1 antibody

Integrin-mediated signaling regulation

Cited 1 time

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Antibodies for α4-integrin immunoprecipitation (PS/2 and HP2/1) and blotting (C-20) were from Millipore, GeneTex, and Santa Cruz Biotechnology, respectively. The phospho-α4-integrin antibody was generated as previously described (22 (link)). PKA subunit antibodies were supplied by Becton Dickinson. An anti–VCAM-1 antibody was from Southern Biotech. H89 was obtained from Millipore, and 8-bromoadenosine cAMP (8-Br-cAMP) was from Enzo Life Sciences. The PRKAR1β cDNA was obtained from GeneCopoeia. Streptozotocin (STZ) and anti-actin and control antibodies were from Sigma-Aldrich.

Abplanalp W.T., Conklin D.J., Cantor J.M., Ginsberg M.H., Wysoczynski M., Bhatnagar A, & O’Toole T.E. (2016). Enhanced Integrin α4β1–Mediated Adhesion Contributes to a Mobilization Defect of Endothelial Progenitor Cells in Diabetes. Diabetes, 65(11), 3505-3515.

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Paraformaldehyde Perfusion and VCAM-1 Immunofluorescence

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Mice were perfused through the left ventricle with 4% (wt/vol) paraformaldehyde as described [26] (link). Briefly, the aortic root was embedded in OCT medium, frozen and cut in 10 μm-sections onto Fisher Superfrost Plus-coated slides, fixed and incubated with anti-VCAM-1 antibody (Southern Biotech; 1:100) followed by anti-rat Alexa-fluor 555 (Cell Signaling, 1:2000, 1 h). In the negative control, VCAM-1 antibody was replaced by an equivalent dilution of normal rat serum (NRS).

Russo L., Muturi H.T., Ghadieh H.E., Wisniewski A.M., Morgan E.E., Quadri S.S., Landesberg G.P., Siragy H.M., Vazquez G., Scalia R., Gupta R, & Najjar S.M. (2018). Liver-specific rescuing of CEACAM1 reverses endothelial and cardiovascular abnormalities in male mice with null deletion of Ceacam1 gene. Molecular Metabolism, 9, 98-113.

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