Human cells were isolated from surplus liver tissue of a one-year-old girl with fulminant hepatitis (Hep2064). Primary cells were isolated according to the collagenase perfusion method25 (link),29 (link),30 (link). iPSC-O cells were generated by introduction of Sendai virus carrying the 4 Yamanaka factors31 (link). The iPSC-O cells were cultured on MEFs and grown using a medium for human ES cells [KNOCKOUT DMEM (Gibco, 10829), 15% CTS KNOCKOUT SR XenoFree Medium (Gibco, 14150), 1% penicillin streptomycin (Gibco, 11140), 1% non-essential amino acids (Gibco, 11360), 1% Sodium Pyruvate (Gibco, 11360), 1% Gulta MAX (Gibco, 35050), 0.1% β-mercaptoethanol (Gibco, 21985-023), and 10.0 ng/ml bFGF (INVITROGEN, PHG0024)].
Cts knockout sr xenofree medium
Manufactured by Thermo Fisher Scientific
The CTS KNOCKOUT SR XenoFree Medium is a serum-free, xeno-free cell culture medium designed for the expansion and differentiation of pluripotent stem cells. The medium provides essential nutrients and growth factors required for the maintenance and proliferation of these specialized cell types.
Automatically generated - may contain errors
Lab products found in correlation
Knockout dmem, by Thermo Fisher Scientific (1 mentions)
Gultamax, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Sodium pyruvate, by Thermo Fisher Scientific (1 mentions)
Non essential amino acids (neaa), by Thermo Fisher Scientific (1 mentions)
Basic fibroblast growth factor (bfgf), by Thermo Fisher Scientific (1 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
Cryotube, by Thermo Fisher Scientific (1 mentions)
Coolcell freezing container, by Corning (1 mentions)
Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Thermo Fisher Scientific (1 mentions)
Nutristem hpsc xf medium, by Sartorius (1 mentions)
2 protocols using cts knockout sr xenofree medium
1
Isolation and Culture of iPSC-O Cells
2
Cryopreservation of Stem Cells
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The cells were rinsed with phosphate-buffered saline (PBS, Gibco), non-enzymatically passaged by 0.5 mM of EDTA (Invitrogen), and spined for 4 min/300 g. Cells (0.5 × 106/mL) were resuspended in cold freezing medium consisting of 65% NutriStem® hPSC XF Medium (Biological Industries), 25% CTS Knockout SR XenoFree Medium (Gibco), and 10% CryoSure-DMSO (WAK chemie) supplemented with 10 µM ROCK inhibitor and frozen in cryotubes (Nunc) placed in Corning CoolCell Freezing container at the rate of -1 °C/minute in −80°C freezer. cryotubes were moved to nitrogen vapors (− 196 °C) after 24 h.
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