Amlodipine and gefitinib were purchased from Selleck Chemicals (Houston, TX, United States) and TopScience (Shanghai, China), respectively. Propidium iodide (PI) was obtained from Sigma-Aldrich (St. Louis, MO, United States). The Annexin V/FITC-PI apoptosis detection kit was obtained from BD Biosciences (San Jose, CA, United States of America). RPMI 1640 and FBS were purchased from Biological Industries (Beit Haemek, Israel). The enhanced chemiluminescence (ECL) reagent was purchased from Thermo Fisher Scientific (Waltham, MA, United States). Antibodies against phospho-EGFR, phospho-PDK1 (Ser241), PDK1, phospho-Akt (Ser473), phospho-Akt (Thr308), Akt, phospho-mammalian target of rapamycin complex 1 (mTORC1) (Ser2448), mTOR, phospho-glycogen synthase kinase 3 (GSK-3) β, phospho-p70 S6K (Thr389), p27, cyclin D1, phospho-retinoblastoma protein (Rb), phospho-extracellular signal-regulated kinase (ERK) 1/2, phospho-c-Raf, β-actin, and horseradish peroxidase (HRP)-conjugated goat anti-rabbit and horse anti-mouse secondary antibodies were purchased from Cell Signaling Technology Inc. (Danvers, MA, United States). Antibody specific for p21 was obtained from Santa Cruz Biotechnology Inc. (Dallas, TX, United States).
Amlodipine
Manufactured by Selleck Chemicals
Sourced in United States
Amlodipine is a laboratory instrument used for the analysis and measurement of chemical compounds. It is a type of high-performance liquid chromatography (HPLC) instrument, which is a widely used analytical technique in various research and testing applications.
Automatically generated - may contain errors
Lab products found in correlation
Gefitinib, by Selleck Chemicals (1 mentions)
Cyclin d1, by Cell Signaling Technology (1 mentions)
Enhanced chemiluminescence ecl reagent, by Thermo Fisher Scientific (1 mentions)
Phospho c raf, by Cell Signaling Technology (1 mentions)
Phospho retinoblastoma protein rb, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase hrp conjugated goat anti rabbit, by Cell Signaling Technology (1 mentions)
Horse anti mouse secondary antibody, by Cell Signaling Technology (1 mentions)
Antibody specific for p21, by Santa Cruz Biotechnology (1 mentions)
Rpmi 1640, by Sartorius (1 mentions)
Phospho p70s6k, by Cell Signaling Technology (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Erk1 2, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Propidium iodide, by Merck Group (1 mentions)
Annexin 5 fitc pi apoptosis detection kit, by BD (1 mentions)
Astemizole, by Bio-Techne (1 mentions)
Lisinopril, by Fujifilm (1 mentions)
Gentamycin, by Fujifilm (1 mentions)
Mibefradil, by Merck Group (1 mentions)
Atorvastatin, by LGC (1 mentions)
2 protocols using amlodipine
1
Amlodipine and Gefitinib Apoptosis Assay
2
Drug Compound Sourcing and Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Ambrisentan, amlodipine, bisoprolol, candesartan, linagliptin, naftopidil, prasugrel, and sumatriptan were purchased from Selleck Chemicals (Houston, TX, USA). Clonidine, glyburide, lisinopril, nicorandil, and propafenone were obtained from Wako (Tokyo, Japan). Efonidipine, mibefradil, terfenadine, and terodiline were provided by Sigma-Aldrich (St. Louis, MO, USA). Aliskiren and warfarin were from LKT Laboratories (St. Paul, MN, USA); astemizole and bepridil were from Tocris Bioscience (Bristol, UK); amiodarone and atorvastatin were from Toronto Research Chemicals (Toronto, Canada); cisapride was from Abcam (Cambridge, UK); and ketotifen was from Tokyo Chemical Industry (Tokyo, Japan). All compounds were dissolved in a mixture of dimethyl sulfoxide (DMSO) and methanol (7:3), and were stored at -80°C. For cell culture, gentamycin and gelatin (type A, cell culture tested) were obtained from Wako and Sigma-Aldrich, respectively.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!