The lipids in the liver were extracted by use of isopropanol/chloroform/NP-40 (11:7:0.1, v/v). After drying, the extracted lipids were dissolved by using the commercial solutions and the levels of total cholesterol, free cholesterol, cholesteryl ester, triglycerides, fatty acids, and glycerol were measured by using fluorescence assay kits (BioVision, Milpitas, CA, USA). The fluorescence intensity was detected at 535 nm excitation and 590 nm emission under the multimode microplate reader Infinite 200 (TECAN, Männedorf, Switzerland).
Fluorescence assay kits
Manufactured by Abcam
Sourced in United States
Fluorescence assay kits are laboratory tools designed to detect and quantify specific analytes, such as proteins, enzymes, or metabolites, using fluorescence-based detection methods. These kits provide a standardized and optimized set of reagents and protocols to perform fluorescence-based assays in a reliable and reproducible manner.
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Lab products found in correlation
6 protocols using fluorescence assay kits
1
Quantitative Hepatic Lipid Profiling
2
Cholesterol and Lipid Evaluation Protocol
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The levels of total cholesterol, free cholesterol, cholesteryl ester, triglycerides, fatty acids, and glycerol were evaluated as previously described [7 (link),9 (link),10 (link)]. They were evaluated using fluorescence assay kits (BioVision, Milpitas, CA, USA) according to the manufacturer’s instructions.
3
Quantitative Liver Lipid Analysis
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The lipids in the liver were extracted by use of n-hexane/isopropanol (3:2, v/v) for 2 h on a shaker. After drying, the extracted lipids were dissolved by using the commercial solutions, and the levels of intracellular total cholesterol, cholesteryl ester, triglycerides, free fatty acids, and glycerol were assessed by using fluorescence assay kits (BioVision, Milpitas, CA, USA). The fluorescence intensity of cell lysates was analyzed at 530 nm excitation and 590 nm emission wavelengths by SpectraMax i3x fluorometry (San Jose, CA, USA).
4
Evaluating Antioxidant Profiles in Bromelain-Treated ApoE-/- Mice
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The levels of total antioxidant capacity, antioxidant proteins, and small molecules in the livers and aortas of bromelain-treated apoe−/− mice were evaluated using fluorescence assay kits (BioVision, Milpitas, CA, USA) according to the manufacturer’s instructions.
5
Fluorescence Assay of Lipid Metabolites
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The levels of total cholesterol, free cholesterol, cholesteryl ester, triglycerides, fatty acids, and glycerol were measured using fluorescence assay kits (BioVision, Milpitas, CA, USA).
6
Intracellular Fatty Acid Quantification
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The lipids in the cells were extracted by use of n-hexane/isopropanol (3:2, v/v) for 1 h on a shaker. After drying, the extracted lipids were dissolved by using the commercial solutions, and the levels of intracellular free fatty acids were assessed by using fluorescence assay kits (BioVision, Milpitas, CA, USA). The fluorescence intensity of cell lysates was analyzed at 530 nm excitation and 590 nm emission wavelengths by SpectraMax i3x fluorometry (San Jose, CA, USA).
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