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Vegf immunoassay kit

Manufactured by R&D Systems
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The VEGF immunoassay kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of Vascular Endothelial Growth Factor (VEGF) levels in cell culture supernates, serum, and plasma. The kit includes a microplate pre-coated with an antibody specific to VEGF, a biotin-conjugated antibody for detection, and necessary reagents for performing the assay.

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VEGF enzyme-linked immunoassay kits Mouse VEGF Immunoassay kit Quantikine mouse VEGF immunoassay ELISA kit Quantikine Human VEGF Immunoassay kit VEGF-C enzyme immunoassay kit Quantikine Mouse VEGF Immunoassay kit Human VEGF immunoassay kit Quantikine Human VEGF Immunoassay ELISA kit

8 protocols using vegf immunoassay kit

1

VEGF Quantification in Treated Cells

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The VEGF concentration in the media from the cells treated with 1 and 2 (25, 50 μM) was determined with a VEGF immunoassay kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. The results were expressed as the concentration of VEGF relative to the total amount of protein from each well.
Jang J.P., Jung H.J., Han J.M., Jung N., Kim Y., Kwon H.J., Ko S.K., Soung N.K., Jang J.H, & Ahn J.S. (2017). Two cyclic hexapeptides from Penicillium sp. FN070315 with antiangiogenic activities. PLoS ONE, 12(9), e0184339.
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2

VEGF Quantification in AMP-Treated Cells

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VEGF concentration in the media from the AMP-treated cells was determined using a VEGF Immunoassay kit (R&D Systems, USA) according to the manufacturer’s instructions. The results are expressed as the concentration of VEGF relative to the total amount of VEGF from each well.
Jung H.J., Kim Y., Lee H.B, & Kwon H.J. (2014). Antiangiogenic Activity of the Lipophilic Antimicrobial Peptides from an Endophytic Bacterial Strain Isolated from Red Pepper Leaf. Molecules and Cells, 38(3), 273-278.
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3

Quantifying VEGF and PEDF in H1299 cells

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H1299 cells were seeded in 96-well plates and treated with TFAP2B siRNA at 100 nM for 48 hours. The VEGF and PEDF levels in the culture media were quantified using a VEGF Immunoassay Kit (968962) (R & D Systems, Minneapolis, MN) and a Chemikine PEDF ELISA Kit (CYT420) (Chemikine, Billerica, MA) according to the manufacturer’s protocols.
Fu L., Shi K., Wang J., Chen W., Shi D., Tian Y., Guo W., Yu W., Xiao X., Kang T., Wang S., Huang W, & Deng W. (2014). TFAP2B overexpression contributes to tumor growth and a poor prognosis of human lung adenocarcinoma through modulation of ERK and VEGF/PEDF signaling. Molecular Cancer, 13, 89.
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4

VEGF Quantification in U87MG Cells

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The VEGF concentration in the media from the U87MG cells treated with elaiophylin (50–200 nM) was determined using a VEGF immunoassay kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions. The results were expressed as the concentration of VEGF relative to the total amount of protein from each well.
Lim H.N., Jang J.P., Han J.M., Jang J.H., Ahn J.S, & Jung H.J. (2018). Antiangiogenic Potential of Microbial Metabolite Elaiophylin for Targeting Tumor Angiogenesis. Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry, 23(3), 563.
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5

VEGF Immunoassay Protocol

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Aliquots of medium from each transfected well were collected and levels of VEGF were measured with a VEGF Immunoassay kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions (Sun et al. 2009 (link)).
Gao L., Li S., Wei X., Du G., Wei D, & Wei L. (2020). Conditional deletion of HDAC4 from collagen type 2α1-expressing cells increases angiogenesis in vivo. Molecular Medicine, 26, 36.
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6

VEGF Secretion in Cancer and Endothelial Cells

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VEGF secretion was measured in MCF-7 cells and HUVECs using a VEGF immunoassay kit (R&D Systems, Inc., inneapolis, MN, USA). Cells were incubated with vehicle or CA4 (10 nM) for 24 h under indicated conditions. Supernatants were collected and the levels of VEGF protein were measured according to the manufacturer’s protocol.
Su M., Huang J., Liu S., Xiao Y., Qin X., Liu J., Pi C., Luo T., Li J., Chen X, & Luo Z. (2016). The anti-angiogenic effect and novel mechanisms of action of Combretastatin A-4. Scientific Reports, 6, 28139.
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7

VEGF Quantification from Tricin-Treated Cells

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VEGF concentration in the media from the tricin-treated cells was determined using a VEGF immunoassay kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. The results were expressed as the concentration of VEGF relative to the total amount of protein from each well.
Han J.M., Kwon H.J, & Jung H.J. (2016). Tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, exhibits potent antiangiogenic activity in vitro. International journal of oncology, 49(4).
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8

VEGF Quantification in Hovenia dulcis

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Hovenia dulcis Thunb. extract-or ampelopsin-treated cells was determined using a VEGF immunoassay kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. The results were expressed as the concentration of VEGF relative to the total amount of protein from each well.
Han J.M., Lim H.N, & Jung H.J. (2017). Hovenia dulcis Thunb. and its active compound ampelopsin inhibit angiogenesis through suppression of VEGFR2 signaling and HIF-1α expression. Oncology reports, 38(6).
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